3-(1h-benzo[d]imidazol-2-yl)-1h-pyrazolo[3,4-c]pyridine and therapeutic uses thereof

ABSTRACT

Azaindazole compounds for treating various diseases and pathologies are disclosed. More particularly, the present invention concerns the use of an azaindazole compound or analogs thereof, in the treatment of disorders characterized by the activation of Wnt pathway signaling (e.g., cancer, abnormal cellular proliferation, angiogenesis, fibrotic disorders, bone or cartilage diseases, and osteoarthritis), the modulation of cellular events mediated by Wnt pathway signaling, as well as genetic diseases and neurological conditions/disorders/diseases due to mutations or dysregulation of the Wnt pathway and/or of one or more of Wnt signaling components. Also provided are methods for treating Wnt-related disease states.

RELATED APPLICATIONS

This application is a continuation of U.S. application Ser. No. 15/591,566, filed May 10, 2017, which is a continuation of U.S. application Ser. No. 14/847,299, filed Sep. 8, 2015, which claims the benefit of U.S. Provisional Application No. 62/047,509, filed Sep. 8, 2014, each of which is incorporated herein by reference in its entirety.

BACKGROUND Technical Field

This disclosure relates to inhibitors of one or more proteins in the Wnt pathway, including inhibitors of one or more Wnt proteins, and compositions comprising the same. More particularly, it concerns the use of an azaindazole compound or salts or analogs thereof, in the treatment of disorders characterized by the activation of Wnt pathway signaling (e.g., cancer, abnormal cellular proliferation, angiogenesis, fibrotic disorders, bone or cartilage diseases, and osteoarthritis), the modulation of cellular events mediated by Wnt pathway signaling, as well as genetic diseases and neurological conditions/disorders/diseases due to mutations or dysregulation of the Wnt pathway and/or of one or more of Wnt signaling components. Also provided are methods for treating Wnt-related disease states.

Background

The Wnt growth factor family includes more than 10 genes identified in the mouse and at least 19 genes identified in the human. Members of the Wnt family of signaling molecules mediate many short- and long-range patterning processes during invertebrate and vertebrate development. The Wnt signaling pathway is known for its role in the inductive interactions that regulate growth and differentiation, and it also plays roles in the homeostatic maintenance of post-embryonic tissue integrity. Wnt stabilizes cytoplasmic 3-catenin, which stimulates the expression of genes including c-myc, c jun, fra-1, and cyclin Dl. In addition, misregulation of Wnt signaling can cause developmental defects and is implicated in the genesis of several human cancers. The Wnt pathway has also been implicated in the maintenance of stem or progenitor cells in a growing list of adult tissues including skin, blood, gut, prostate, muscle, and the nervous system.

SUMMARY

The present disclosure provides methods and reagents, involving contacting a cell with an agent, such as an azaindazole compound, in a sufficient amount to antagonize a Wnt activity, e.g., to reverse or control an aberrant growth state or correct a genetic disorder due to mutations in Wnt signaling components.

Some embodiments disclosed herein include Wnt inhibitors containing an azaindazole core. Other embodiments disclosed herein include pharmaceutical compositions and methods of treatment using these compounds.

One embodiment disclosed herein includes a compound having the structure of Formula I:

as well as prodrugs and pharmaceutically acceptable salts thereof.

In some embodiments of Formula (I):

R¹ is selected from the group consisting of -heteroaryl(R⁴)_(q) and -heterocyclyl(R⁵)_(h);

R² is selected from the group consisting of H and halide;

R³ is selected from the group consisting of H, -heteroaryl(R⁶)_(q), -heterocyclyl(R⁶)_(h), and -aryl(R⁸)_(k);

each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of halide, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R⁹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R¹⁰)_(j), —(C₁₋₄ alkylene)_(p)aryl(R¹¹)_(k), —NHC(═O)R¹², —NR¹³R¹⁴, —(C₁₋₆ alkylene)NR¹⁵R¹⁶, and —OR²²;

each R⁵ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷;

each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —CN, and —OCH₃;

each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —CN, —OCH₃, —(C₁₋₆ alkylene)_(p)NHSO₂R¹⁷, —NR¹³(C₁₋₆ alkylene)NR¹³R¹⁴, —(C₁₋₆ alkylene)_(p)NR¹³R¹⁴, and —OR²⁵;

each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R¹² is independently selected from the group consisting of —(C₁₋₉ alkyl), -heteroaryl(R¹⁸)_(q), -aryl(R¹⁹)_(k), —CH₂aryl(R¹⁹)_(k), -carbocyclyl(R²⁰)_(j), —CH₂carbocyclyl(R²⁰)_(j), —(C₁₋₄ alkylene)_(p)NR²³R²⁴, -heterocyclyl(R²¹)_(h), and —CH₂heterocyclyl(R²¹)_(h);

each R¹³ is independently selected from the group consisting of H and —(C₁₋₆ alkyl);

each R¹⁴ is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —CH₂aryl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰);

each R¹⁵ is independently selected from the group consisting of H and —(C₁₋₆ alkyl);

each R¹⁶ is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —CH₂aryl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰);

each R¹⁷ is a —(C₁₋₆ alkyl);

each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN;

each R²² is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R²¹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R²⁰), —(C₁₋₄ alkylene)_(p)aryl(R¹⁹)_(k), and —(C₁₋₆ alkylene)_(p)NR²³R²⁴;

each R²³ is independently selected from the group consisting of H and —(C₁₋₆ alkyl);

each R²⁴ is independently selected from the group consisting of H and —(C₁₋₆ alkyl);

each R²⁵ is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R²¹)_(h), and —(C₁₋₆ alkylene)_(p)NR²³R²⁴;

each p is independently 0 or 1;

each q is independently 0 to 4;

each h is independently 0 to 10;

each k is independently 0 to 5; and

each j is independently 0 to 12.

Some embodiments include stereoisomers and pharmaceutically acceptable salts of a compound of Formula (I).

Some embodiments include pro-drugs of a compound of Formula (I).

Some embodiments of the present disclosure include pharmaceutical compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier, diluent, or excipient.

Other embodiments disclosed herein include methods of inhibiting one or more members of the Wnt pathway, including one or more Wnt proteins by administering to a patient affected by a disorder or disease in which aberrant Wnt signaling is implicated, such as cancer and other diseases associated with abnormal angiogenesis, cellular proliferation, cell cycling and mutations in Wnt signaling components, a compound according to Formula (I). Accordingly, the compounds and compositions provided herein can be used to treat cancer, to reduce or inhibit angiogenesis, to reduce or inhibit cellular proliferation and correct a genetic disorder due to mutations in Wnt signaling components.

Non-limiting examples of diseases which can be treated with the compounds and compositions provided herein include a variety of cancers, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, sepsis, ankylosing spondylitis, psoriasis, scleroderma, mycotic and viral infections, osteochondrodysplasia, Alzheimer's disease, lung disease, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), polyposis coli, osteoporosis-pseudoglioma syndrome, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-Amelia syndrome, Millerian-duct regression and virilization, SERKAL syndrome, diabetes mellitus type 2, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication syndrome, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease, and Rett syndrome.

Some embodiments of the present disclosure include methods to prepare compounds of Formula (I).

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.

DETAILED DESCRIPTION

Provided herein are compositions and methods for inhibiting one or more members of the Wnt pathway, including one or more Wnt proteins. Other Wnt inhibitors and methods for using the same are disclosed in U.S. application Ser. Nos. 12/852,706; 12/968,505; 13/552,188; 13/800,963; 13/855,874; 13/887,177 13/938,691; 13/938,692; 14/019,103; 14/019,147; 14/019,940; 14/149,948; 14/178,749; 14/331,427; and Ser. No. 14/334,005; and U.S. Provisional Application Ser. Nos. 61/232,603; 61/288,544; 61/305,459; 61/620,107; 61/642,915; and 61/750,221, all of which are incorporated by reference in their entirety herein.

Some embodiments provided herein relate to a method for treating a disease or disorder including, but not limited to, cancers, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, sepsis, ankylosing spondylitis, psoriasis, scleroderma, mycotic and viral infections, bone and cartilage diseases, Alzheimer's disease, lung disease, osteoarthritis, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), polyposis coli, bone density and vascular defects in the eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye diseases or syndromes associated with defects or damaged photoreceptors, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia, Müllerian-duct regression and virilization, SERKAL syndrome, type II diabetes, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease, and Rett syndrome.

In some embodiments, non-limiting examples of bone and cartilage diseases which can be treated with the compounds and compositions provided herein include bone spur (osteophytes), craniosynostosis, fibrodysplasia ossificans progressive, fibrous dysplasia, giant cell tumor of bone, hip labral tear, meniscal tears, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), osteochondritis dissecans, osteochondroma (bone tumor), osteopetrosis, relapsing polychondritis, and Salter-Harris fractures.

In some embodiments, pharmaceutical compositions are provided that are effective for treatment of a disease of an animal, e.g., a mammal, caused by the pathological activation or mutations of the Wnt pathway. The composition includes a pharmaceutically acceptable carrier and a compound as described herein.

Definitions

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this disclosure belongs. All patents, applications, published applications, and other publications are incorporated by reference in their entirety. In the event that there is a plurality of definitions for a term herein, those in this section prevail unless stated otherwise.

As used herein, “alkyl” means a branched, or straight chain chemical group containing only carbon and hydrogen, such as methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, tert-butyl, n-pentyl, iso-pentyl, sec-pentyl and neo-pentyl. Alkyl groups can either be unsubstituted or substituted with one or more substituents. Alkyl groups can be saturated or unsaturated (e.g., containing —C═C— or —C≡C— subunits), at one or several positions. In some embodiments, alkyl groups include 1 to 9 carbon atoms (for example, 1 to 6 carbon atoms, 1 to 4 carbon atoms, or 1 to 2 carbon atoms).

As used herein, “alkylene” means a bivalent branched, or straight chain chemical group containing only carbon and hydrogen, such as methylene, ethylene, n-propylene, iso-propylene, n-butylene, iso-butylene, sec-butylene, tert-butylene, n-pentylene, iso-pentylene, sec-pentylene and neo-pentylene. Alkylene groups can either be unsubstituted or substituted with one or more substituents. Alkylene groups can be saturated or unsaturated (e.g., containing —C═C— or —C≡C— subunits), at one or several positions. In some embodiments, alkylene groups include 1 to 9 carbon atoms (for example, 1 to 6 carbon atoms, 1 to 4 carbon atoms, or 1 to 2 carbon atoms).

As used herein, “carbocyclyl” means a cyclic ring system containing only carbon atoms in the ring system backbone, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cyclohexenyl. Carbocyclyls may include multiple fused rings. Carbocyclyls may have any degree of saturation provided that at least one ring in the ring system is not aromatic. Carbocyclyl groups can either be unsubstituted or substituted with one or more substituents. In some embodiments, carbocyclyl groups include 3 to 10 carbon atoms, for example, 3 to 6 carbon atoms.

As used herein, “lower alkyl” means a subset of alkyl having 1 to 3 carbon atoms, which is linear or branched. Examples of lower alkyls include methyl, ethyl, n-propyl and isopropyl. Likewise, radicals using the terminology “lower” refer to radicals having 1 to about 3 carbons in the alkyl portion of the radical.

As used herein, “aryl” means a mono-, bi-, tri- or polycyclic group with only carbon atoms present in the ring backbone having 5 to 14 ring atoms, alternatively 5, 6, 9, or 10 ring atoms; and having 6, 10, or 14 pi electrons shared in a cyclic array; wherein at least one ring in the system is aromatic. Aryl groups can either be unsubstituted or substituted with one or more substituents. Examples of aryl include phenyl, naphthyl, tetrahydronaphthyl, 2,3-dihydro-1H-indenyl, and others. In some embodiments, the aryl is phenyl.

As used herein, “arylalkyl” means an aryl-alkyl- group in which the aryl and alkyl moieties are as previously described. In some embodiments, arylalkyl groups contain a C₁₋₄alkyl moiety. Exemplary arylalkyl groups include benzyl and 2-phenethyl.

As used herein, the term “heteroaryl” means a mono-, bi-, tri- or polycyclic group having 5 to 14 ring atoms, alternatively 5, 6, 9, or 10 ring atoms; and having 6, 10, or 14 pi electrons shared in a cyclic array; wherein at least one ring in the system is aromatic, and at least one ring in the system contains one or more heteroatoms independently selected from the group consisting of N, O, and S. Heteroaryl groups can either be unsubstituted or substituted with one or more substituents. Examples of heteroaryl include thienyl, pyridinyl, furyl, oxazolyl, oxadiazolyl, pyrrolyl, imidazolyl, triazolyl, thiodiazolyl, pyrazolyl, isoxazolyl, thiadiazolyl, pyranyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thiazolyl benzothienyl, benzoxadiazolyl, benzofuranyl, benzimidazolyl, benzotriazolyl, cinnolinyl, indazolyl, indolyl, isoquinolinyl, isothiazolyl, naphthyridinyl, purinyl, thienopyridinyl, pyrido[2,3-d]pyrimidinyl, pyrrolo[2,3-b]pyridinyl, quinazolinyl, quinolinyl, thieno[2,3-c]pyridinyl, pyrazolo[3,4-b]pyridinyl, pyrazolo[3,4-c]pyridinyl, pyrazolo[4,3-c]pyridine, pyrazolo[4,3-b]pyridinyl, tetrazolyl, chromane, 2,3-dihydrobenzo[b][1,4]dioxine, benzo[d][1,3]dioxole, 2,3-dihydrobenzofuran, 2,3-dihydrobenzo[b][1,4]oxathiine, and others. In some embodiments, the heteroaryl is selected from thienyl, pyridinyl, furyl, pyrazolyl, imidazolyl, pyranyl, pyrazinyl, and pyrimidinyl.

As used herein, “halo”, “halide” or “halogen” is a chloro, bromo, fluoro, or iodo atom radical. In some embodiments, a halo is a chloro, bromo or fluoro. For example, a halide can be fluoro.

As used herein, “haloalkyl” means a hydrocarbon substituent, which is a linear or branched, alkyl, alkenyl or alkynyl substituted with one or more chloro, bromo, fluoro, and/or iodo atom(s). In some embodiments, a haloalkyl is a fluoroalkyls, wherein one or more of the hydrogen atoms have been substituted by fluoro. In some embodiments, haloalkyls are of 1 to about 3 carbons in length (e.g., 1 to about 2 carbons in length or 1 carbon in length). The term “haloalkylene” means a diradical variant of haloalkyl, and such diradicals may act as spacers between radicals, other atoms, or between a ring and another functional group.

As used herein, “heterocyclyl” means a nonaromatic cyclic ring system comprising at least one heteroatom in the ring system backbone. Heterocyclyls may include multiple fused rings. Heterocyclyls may be substituted or unsubstituted with one or more substituents. In some embodiments, heterocycles have 5-7 members. In six membered monocyclic heterocycles, the heteroatom(s) are selected from one to three of O, N or S, and wherein when the heterocycle is five membered, it can have one or two heteroatoms selected from O, N, or S. Examples of heterocyclyl include azirinyl, aziridinyl, azetidinyl, oxetanyl, thietanyl, 1,4,2-dithiazolyl, dihydropyridinyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-dioxolanyl, morpholinyl, thiomorpholinyl, piperazinyl, pyranyl, pyrrolidinyl, tetrahydrofuryl, tetrahydropyridinyl, oxazinyl, thiazinyl, thiinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isoxazolidinyl, piperidinyl, pyrazolidinyl imidazolidinyl, thiomorpholinyl, and others. In some embodiments, the heterocyclyl is selected from azetidinyl, morpholinyl, piperazinyl, pyrrolidinyl, and tetrahydropyridinyl.

As used herein, “monocyclic heterocyclyl” means a single nonaromatic cyclic ring comprising at least one heteroatom in the ring system backbone. Heterocyclyls may be substituted or unsubstituted with one or more substituents. In some embodiments, heterocycles have 5-7 members. In six membered monocyclic heterocycles, the heteroatom(s) are selected from one to three of O, N or S, and wherein when the heterocycle is five membered, it can have one or two heteroatoms selected from O, N, or S. Examples of heterocyclyl include azirinyl, aziridinyl, azetidinyl, oxetanyl, thietanyl, 1,4,2-dithiazolyl, dihydropyridinyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-dioxolanyl, morpholinyl, thiomorpholinyl, piperazinyl, pyranyl, pyrrolidinyl, tetrahydrofuryl, tetrahydropyridinyl, oxazinyl, thiazinyl, thiinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isoxazolidinyl, piperidinyl, pyrazolidinyl imidazolidinyl, thiomorpholinyl, and others.

The term “substituted” refers to moieties having substituents replacing a hydrogen on one or more non-hydrogen atoms of the molecule. It will be understood that “substitution” or “substituted with” includes the implicit proviso that such substitution is in accordance with permitted valence of the substituted atom and the substituent, and that the substitution results in a stable compound, e.g., which does not spontaneously undergo transformation such as by rearrangement, cyclization, elimination, etc. Substituents can include, for example, —(C₁₋₉ alkyl) optionally substituted with one or more of hydroxyl, —NH₂, —NH(C₁₋₃ alkyl), and —N(C₁₋₃ alkyl)₂; —(C₁₋₉ haloalkyl); a halide; a hydroxyl; a carbonyl [such as —C(O)OR, and —C(O)R]; a thiocarbonyl [such as —C(S)OR, —C(O)SR, and —C(S)R]; —(C₁₋₉ alkoxyl) optionally substituted with one or more of halide, hydroxyl, —NH₂, —NH(C₁₋₃ alkyl), and —N(C₁₋₃ alkyl)₂; —OPO(OH)₂; a phosphonate [such as —PO(OH)₂ and —PO(OR′)₂]; —OPO(OR′)R¹¹; —NRR′; —C(O)NRR′; —C(NR)NR′R″; —C(NR′)R″; a cyano; a nitro; an azido; —SH; —S—R; —OSO₂(OR); a sulfonate [such as —SO₂(OH) and —SO₂(OR)]; —SO₂NR′R″; and —SO₂R; in which each occurrence of R, R′ and R″ are independently selected from H; —(C₁₋₉ alkyl); C₆₋₁₀ aryl optionally substituted with from 1-3R″′; 5-10 membered heteroaryl having from 1-4 heteroatoms independently selected from N, O, and S and optionally substituted with from 1-3 R″′; C₃₋₇ carbocyclyl optionally substituted with from 1-3 R″′; and 3-8 membered heterocyclyl having from 1-4 heteroatoms independently selected from N, O, and S and optionally substituted with from 1-3 R″′; wherein each R″′ is independently selected from —(C₁₋₆ alkyl), —(C₁₋₆ haloalkyl), a halide (e.g., F), a hydroxyl, —C(O)OR, —C(O)R, —(C₁₋₆ alkoxyl), —NRR′, —C(O)NRR′, and a cyano, in which each occurrence of R and R′ is independently selected from H and —(C₁₋₆ alkyl). In some embodiments, the substituent is selected from —(C₁₋₆ alkyl), —(C₁₋₆ haloalkyl), a halide (e.g., F), a hydroxyl, —C(O)OR, —C(O)R, —(C₁₋₆ alkoxyl), —NRR′, —C(O)NRR′, and a cyano, in which each occurrence of R and R′ is independently selected from H and —(C₁₋₆ alkyl).

As used herein, when two groups are indicated to be “linked” or “bonded” to form a “ring”, it is to be understood that a bond is formed between the two groups and may involve replacement of a hydrogen atom on one or both groups with the bond, thereby forming a carbocyclyl, heterocyclyl, aryl, or heteroaryl ring. The skilled artisan will recognize that such rings can and are readily formed by routine chemical reactions. In some embodiments, such rings have from 3-7 members, for example, 5 or 6 members.

The skilled artisan will recognize that some structures described herein may be resonance forms or tautomers of compounds that may be fairly represented by other chemical structures, even when kinetically, the artisan recognizes that such structures are only a very small portion of a sample of such compound(s). Such compounds are clearly contemplated within the scope of this disclosure, though such resonance forms or tautomers are not represented herein.

The compounds provided herein may encompass various stereochemical forms. The compounds also encompass diastereomers as well as optical isomers, e.g., mixtures of enantiomers including racemic mixtures, as well as individual enantiomers and diastereomers, which arise as a consequence of structural asymmetry in certain compounds. Separation of the individual isomers or selective synthesis of the individual isomers is accomplished by application of various methods which are well known to practitioners in the art. Unless otherwise indicated, when a disclosed compound is named or depicted by a structure without specifying the stereochemistry and has one or more chiral centers, it is understood to represent all possible stereoisomers of the compound.

The term “administration” or “administering” refers to a method of providing a dosage of a compound or pharmaceutical composition to a vertebrate or invertebrate, including a mammal, a bird, a fish, or an amphibian, where the method is, e.g., orally, subcutaneously, intravenously, intralymphatic, intranasally, topically, transdermally, intraperitoneally, intramuscularly, intrapulmonarilly, vaginally, rectally, ontologically, neuro-otologically, intraocularly, subconjuctivally, via anterior eye chamber injection, intravitreally, intraperitoneally, intrathecally, intracystically, intrapleurally, via wound irrigation, intrabuccally, intra-abdominally, intra-articularly, intra-aurally, intrabronchially, intracapsularly, intrameningeally, via inhalation, via endotracheal or endobronchial instillation, via direct instillation into pulmonary cavities, intraspinally, intrasynovially, intrathoracically, via thoracostomy irrigation, epidurally, intratympanically, intracisternally, intravascularly, intraventricularly, intraosseously, via irrigation of infected bone, or via application as part of any admixture with a prosthetic device. The method of administration can vary depending on various factors, e.g., the components of the pharmaceutical composition, the site of the disease, the disease involved, and the severity of the disease.

A “diagnostic” as used herein is a compound, method, system, or device that assists in the identification or characterization of a health or disease state. The diagnostic can be used in standard assays as is known in the art.

The term “mammal” is used in its usual biological sense. Thus, it specifically includes humans, cattle, horses, monkeys, dogs, cats, mice, rats, cows, sheep, pigs, goats, and non-human primates, but also includes many other species.

The term “pharmaceutically acceptable carrier”, “pharmaceutically acceptable diluent” or “pharmaceutically acceptable excipient” includes any and all solvents, co-solvents, complexing agents, dispersion media, coatings, isotonic and absorption delaying agents and the like which are not biologically or otherwise undesirable. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients can also be incorporated into the compositions. In addition, various adjuvants such as are commonly used in the art may be included. These and other such compounds are described in the literature, e.g., in the Merck Index, Merck & Company, Rahway, N.J. Considerations for the inclusion of various components in pharmaceutical compositions are described, e.g., in Gilman et al. (Eds.) (2010); Goodman and Gilman's: The Pharmacological Basis of Therapeutics, 12th Ed., The McGraw-Hill Companies.

The term “pharmaceutically acceptable salt” refers to salts that retain the biological effectiveness and properties of the compounds provided herein and, which are not biologically or otherwise undesirable. In many cases, the compounds provided herein are capable of forming acid and/or base salts by virtue of the presence of amino and/or carboxyl groups or groups similar thereto. Many such salts are known in the art, for example, as described in WO 87/05297. Pharmaceutically acceptable acid addition salts can be formed with inorganic acids and organic acids. Inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like. Organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like. Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases. Inorganic bases from which salts can be derived include, for example, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum, and the like; particularly preferred are the ammonium, potassium, sodium, calcium, and magnesium salts. Organic bases from which salts can be derived include, for example, primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, basic ion exchange resins, and the like, specifically such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, and ethanolamine.

“Solvate” refers to the compound formed by the interaction of a solvent and a compound as provided herein or a salt thereof. Suitable solvates are pharmaceutically acceptable solvates including hydrates.

“Patient” as used herein, means a human or a non-human mammal, e.g., a dog, a cat, a mouse, a rat, a cow, a sheep, a pig, a goat, a non-human primate, or a bird, e.g., a chicken, as well as any other vertebrate or invertebrate. In some embodiments, the patient is a human.

A “therapeutically effective amount” or “pharmaceutically effective amount” of a compound as provided herein is one which is sufficient to achieve the desired physiological effect and may vary according to the nature and severity of the disease condition, and the potency of the compound. “Therapeutically effective amount” is also intended to include one or more of the compounds of Formula I in combination with one or more other agents that are effective to treat the diseases and/or conditions described herein. The combination of compounds can be a synergistic combination. Synergy, as described, for example, by Chou and Talalay, Advances in Enzyme Regulation (1984), 22, 27-55, occurs when the effect of the compounds when administered in combination is greater than the additive effect of the compounds when administered alone as a single agent. In general, a synergistic effect is most clearly demonstrated at sub-optimal concentrations of the compounds. It will be appreciated that different concentrations may be employed for prophylaxis than for treatment of an active disease. This amount can further depend upon the patient's height, weight, sex, age and medical history.

A therapeutic effect relieves, to some extent, one or more of the symptoms of the disease.

“Treat,” “treatment,” or “treating,” as used herein refers to administering a compound or pharmaceutical composition as provided herein for therapeutic purposes. The term “therapeutic treatment” refers to administering treatment to a patient already suffering from a disease thus causing a therapeutically beneficial effect, such as ameliorating existing symptoms, ameliorating the underlying metabolic causes of symptoms, postponing or preventing the further development of a disorder, and/or reducing the severity of symptoms that will or are expected to develop.

“Drug-eluting” and/or controlled release as used herein refers to any and all mechanisms, e.g., diffusion, migration, permeation, and/or desorption by which the drug(s) incorporated in the drug-eluting material pass therefrom over time into the surrounding body tissue.

“Drug-eluting material” and/or controlled release material as used herein refers to any natural, synthetic or semi-synthetic material capable of acquiring and retaining a desired shape or configuration and into which one or more drugs can be incorporated and from which incorporated drug(s) are capable of eluting over time.

“Elutable drug” as used herein refers to any drug or combination of drugs having the ability to pass over time from the drug-eluting material in which it is incorporated into the surrounding areas of the body.

The term “comprising” as used herein is synonymous with “including,” “containing,” or “characterized by,” and is inclusive or open-ended and does not exclude additional, unrecited elements or method steps.

Compounds

The compounds and compositions described herein can be used as anti-proliferative agents, e.g., anti-cancer and anti-angiogenesis agents, and/or as inhibitors of the Wnt signaling pathway, e.g., for treating diseases or disorders associated with aberrant Wnt signaling. In addition, the compounds can be used as inhibitors of one or more kinases, kinase receptors, or kinase complexes. Such compounds and compositions are also useful for controlling cellular proliferation, differentiation, and/or apoptosis.

Some embodiments of the present disclosure include compounds of Formula

or salts, pharmaceutically acceptable salts, or prodrugs thereof.

In some embodiments, R¹ is selected from the group consisting of -pyridinyl(R⁴) and -pyrimidinyl(R⁵).

In some embodiments, R¹ is selected from the group consisting of -heteroaryl(R⁴)_(q) and -heterocyclyl(R⁵)_(h).

In some embodiments, R¹ is selected from the group consisting of -piperidinyl(R⁵)_(h) and -tetrahydropyridinyl(R⁵)_(h).

In some embodiments, R¹ is selected from the group consisting of -pyridinyl(R⁴)_(q), -pyrimidinyl(R⁴)_(q), -pyrazinyl(R⁴)_(q), -pyrazolyl(R⁴)_(q), and -imidazolyl(R⁴)_(q).

In some embodiments, R² is selected from the group consisting of H and halide.

In some embodiments, R³ is selected from the group consisting of -heteroaryl(R⁶)_(q), -heterocyclyl(R⁷)_(h), and -aryl(R⁸)_(k).

In some embodiments, R³ is selected from the group consisting of H, -heteroaryl(R⁶)_(q), -heterocyclyl(R⁷)_(h), and -aryl(R⁸)_(k).

In some embodiments, R³ is selected from the group consisting of -pyridinyl(R⁶)_(q), -imidazolyl(R⁶)_(q), -furanyl(R⁶)_(q), -thiophenyl(R⁶)_(q), -piperidinyl(R⁷)_(h), -piperazinyl(R⁷)_(h), and -phenyl(R⁸)_(k).

In some embodiments, R⁴ is one substituent attached to the pyridinyl and is selected from the group consisting of H, halide, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R⁹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R¹⁰)_(j), —(C₁₋₄ alkylene)_(p)aryl(R¹¹)_(k), —NHC(═O)R¹², —NR¹³R¹⁴, and —(C₁₋₆ alkylene)NR¹⁵R¹⁶.

In some embodiments, each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of halide, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R⁹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R¹⁰), —(C₁₋₄ alkylene)_(p)aryl(R¹¹)_(k), —NHC(═O)R¹², —NR¹³R¹⁴, —(C₁₋₆ alkylene)NR¹⁵R¹⁶, and —OR²².

In some embodiments, each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of F, -Me, -Et, —(CH₂)heterocyclyl(R⁹)_(h), -heterocyclyl(R⁹)_(h), —(CH₂)carbocyclyl(R¹⁰), —(CH₂)aryl(R¹¹)_(k), —NHC(═O)(C₁₋₅ alkyl), —NHC(═O)phenyl(R¹⁹)_(k), —NHC(═O)(CH₂)phenyl(R¹⁹)_(k), —NHC(═O)carbocyclyl(R²⁰)_(j), —NHC(═O)(CH₂)heterocyclyl(R²¹)_(h), —NH₂, —N(C₁₋₃ alkyl)₂, —NH(C₁₋₄ alkyl), —(CH₂)N(C₁₋₃ alkyl)₂, —(CH₂)NH(C₁₋₄ alkyl), —OH, —O(C₁₋₃ alkyl), -Ocarbocyclyl(R²⁰)_(j), -Oheterocyclyl(R²¹)_(h), —O(CH₂CH₂)heterocyclyl(R²¹)_(h), —O(CH₂CH₂)N(C₁₋₃ alkyl)₂, and —O(CH₂)phenyl(R¹⁹)_(k).

In some embodiments, R⁵ is one substituent attached to the pyrimidinyl and is selected from the group consisting of H, halide, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R⁹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R¹⁰)_(j), —(C₁₋₄ alkylene)_(p)aryl(R¹)_(k), —NHC(═O)R¹², —NR¹³R¹⁴, and —(C₁₋₆ alkylene)NR¹⁵R¹⁶.

In some embodiments, each R⁵ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of H, —(C₁₋₆ alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷.

In some embodiments, each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷.

In some embodiments, each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of -Me, -Et, F, —CF₃, —OCH₃, —CN, and —C(═O)(C₁₋₃ alkyl).

In some embodiments, each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of H, —(C₁₋₆ alkyl), halide, —CF₃, —CN, and —OCH₃.

In some embodiments, each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —CN, and —OCH₃.

In some embodiments, each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of H, —(C₁₋₆ alkyl), halide, —CF₃, —CN, —OCH₃, —(C₁₋₆ alkylene)_(p)NHSO₂R¹⁷, —NR¹³(C₁₋₆ alkylene)NR¹³R¹⁴, and —(C₁₋₆ alkylene)_(p)NR¹³R¹⁴.

In some embodiments, each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —CN, —OCH₃, —(C₁₋₆ alkylene)_(p)NHSO₂R¹⁷, —NR¹³(C₁₋₆ alkylene)NR¹³R¹⁴, —(C₁₋₆ alkylene)_(p)NR¹³R¹⁴, and —OR²⁵.

In some embodiments, each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of -Me, -Et, F, —CF₃, —CN, —OCH₃, —(CH₂CH₂)NHSO₂(C₁₋₃ alkyl), —NH(CH₂CH₂)N(C₁₋₃ alkyl)₂, —OH, —O(C₁₋₃ alkyl), —O(CH₂CH₂)heterocyclyl(R²¹)_(h), and —O(CH₂CH₂)N(C₁₋₃ alkyl)₂.

In some embodiments, each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of H, —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of H, —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of H, —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹² is independently selected from the group consisting of —(C₁₋₉ alkyl), -heteroaryl(R¹⁸)_(q), -aryl(R¹⁹)_(k), —CH₂aryl(R¹⁹)_(k), -carbocyclyl(R²⁰), and —CH₂carbocyclyl(R²⁰)_(j).

In some embodiments, each R¹² is independently selected from the group consisting of —(C₁₋₉ alkyl), -heteroaryl(R¹⁸)_(q), -aryl(R¹⁹)_(k), —CH₂aryl(R¹⁹)_(k), -carbocyclyl(R²⁰)_(j), —CH₂carbocyclyl(R²⁰)_(j), —(C₁₋₄ alkylene)_(p)NR²³R²⁴, -heterocyclyl(R²¹)_(h), and —CH₂heterocyclyl(R²¹)_(h).

In some embodiments, each R¹² is independently selected from the group consisting of —(C₁₋₅ alkyl), -phenyl(R¹⁹)_(k), —(CH₂)phenyl(R¹⁹)_(k), -carbocyclyl(R²⁰), —(CH₂)carbocyclyl(R²⁰)_(j), —(CH₂)N(C₁₋₃ alkyl)₂, and —(CH₂)heterocyclyl(R²¹)_(h).

In some embodiments, each R¹³ is independently selected from the group consisting of H and —(C₁₋₆ alkyl).

In some embodiments, each R¹³ is independently selected from the group consisting of H and —(C₁₋₃ alkyl).

In some embodiments, each R¹⁴ is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —CH₂aryl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰).

In some embodiments, each R¹⁴ is independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰).

In some embodiments, each R¹⁵ is independently selected from the group consisting of H and —(C₁₋₆ alkyl).

In some embodiments, each R¹⁵ is independently selected from the group consisting of H and —(C₁₋₃ alkyl).

In some embodiments, each R¹⁶ is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —CH₂aryl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰).

In some embodiments, each R¹⁶ is independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰).

In some embodiments, each R¹⁷ is independently a —(C₁₋₆ alkyl).

In some embodiments, each R¹⁷ is independently a —(C₁₋₃ alkyl).

In some embodiments, each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of H, —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of H, —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of H, —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN.

In some embodiments, each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of Me, Et, F, Cl, and —CF₃.

In some embodiments, R²² is selected from the group consisting of H, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R²¹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R²⁰)_(j), —(C₁₋₄ alkylene)_(p)aryl(R¹⁹)_(k), and —(C₁₋₆ alkylene)_(p)NR²³R²⁴.

In some embodiments, R²² is selected from the group consisting of H, -Me, -Et, -iPr, -heterocyclyl(R²)_(h), —(CH₂CH₂)heterocyclyl(R²)_(h), -carbocyclyl(R²⁰)_(j), —(CH₂)phenyl(R¹⁹)_(k), and —(CH₂CH₂)N(C₁₋₃ alkyl)₂.

In some embodiments, each R²³ is independently selected from the group consisting of H and —(C₁₋₆ alkyl).

In some embodiments, each R²³ is independently selected from the group consisting of Me and Et.

In some embodiments, each R²⁴ is independently selected from the group consisting of H and —(C₁₋₆ alkyl).

In some embodiments, each R²⁴ is independently selected from the group consisting of Me and Et.

In some embodiments, R²⁵ is selected from the group consisting of H, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R²¹)_(h), and —(C₁₋₆ alkylene)_(p)NR²³R²⁴.

In some embodiments, R²⁵ is selected from the group consisting of H, -Me, -Et, -iPr, —(CH₂CH₂)heterocyclyl(R²¹)_(h), and —(CH₂CH₂)N(C₁₋₃ alkyl)₂.

In some embodiments, each p is independently 0 or 1.

In some embodiments, each q is independently 1 to 4.

In some embodiments, each h is independently 1 to 10.

In some embodiments, each k is independently 1 to 5.

In some embodiments, each j is independently 1 to 12.

In some embodiments, each p is independently 0 or 1; in some embodiments, each p is 0; in some embodiments, each p is 1.

In some embodiments, each q is independently 0 to 4; in some embodiments, each q is 0; in some embodiments, each q is 1; in some embodiments, each q is 2; in some embodiments, each q is 3; in some embodiments, each q is 4.

In some embodiments, each h is independently 0 to 10; in some embodiments, each h is 0; in some embodiments, each h is 1; in some embodiments, each h is 2; in some embodiments, each h is 3; in some embodiments, each h is 4.

In some embodiments, each k is independently 0 to 5; in some embodiments, each k is 0; in some embodiments, each k is 1; in some embodiments, each k is 2; in some embodiments, each k is 3.

In some embodiments, each j is independently 0 to 12; in some embodiments, each j is 0; in some embodiments, each j is 1; in some embodiments, each j is 2; in some embodiments, each j is 3; in some embodiments, each j is 4.

In some embodiments, each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C₁₋₃ alkyl), —CH₂heterocyclyl(R⁹)_(h), —NHC(═O)R¹², —NR¹³R¹⁴, and —CH₂NR¹⁵R¹⁶.

In some embodiments, at least one R⁹ is halide.

In some embodiments, R¹² is selected from the group consisting of —(C₁₋₅ alkyl), -phenyl(R¹⁹)_(k), —CH₂phenyl(R¹⁹)_(k), and -carbocyclyl(R²⁰)_(j).

In some embodiments, R¹³ and R¹⁴ are independently selected from H and —(C₁₋₅ alkyl).

In some embodiments, R¹⁵ and R¹⁶ are independently selected from H and —(C₁₋₅ alkyl).

In some embodiments, k is 1 or 2 and each R⁸ is independently a halide.

In some embodiments, k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷.

In some embodiments, R¹⁷ is —(C₁₋₃ alkyl).

In some embodiments, k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴.

In some embodiments, R¹³ and R¹⁴ are independently selected from H and —(C₁₋₃ alkyl).

In some embodiments, R³ is selected from the group consisting of -pyridinyl(R⁶)_(q), -imidazolyl(R⁶)_(q), -furanyl(R⁶)_(q), and -thiophenyl(R⁶)_(q).

In some embodiments, q is 0 or 1, R⁶ is selected from the group consisting of halide, —(C₁₋₃ alkyl), and —C(═O)R¹⁷, wherein R¹⁷ is —(C₁₋₂ alkyl).

In some embodiments, R³ is selected from the group consisting of -piperidinyl(R⁷)_(h) and -piperazinyl(R⁷)_(h).

In some embodiments, q is 1, and R′ is selected from the group consisting of H and —(C₁₋₃ alkyl).

In some embodiments, R² is H; in other embodiments, R² is halide, e.g. F.

In some embodiments, R¹ is -heteroaryl(R⁴)_(q).

In some embodiments, R¹ is -pyridinyl(R⁴)_(q).

In some embodiments, R¹ is -pyridin-3-yl(R⁴)_(q).

In some embodiments, R¹ is -pyrimidinyl(R⁴)_(q).

In some embodiments, R¹ is -pyrimidin-5-yl(R⁴)_(q).

In some embodiments, R¹ is -pyrimidin-5-yl(R⁴)_(q) and q is 0.

In some embodiments, R¹ is -pyrazinyl(R⁴)_(q).

In some embodiments, R¹ is -pyrazolyl(R⁴)_(q).

In some embodiments, R¹ is -pyrazol-4-yl(R⁴)_(q), q is 1, and R⁴ is Me.

In some embodiments, R¹ is -pyrazol-4-yl(R⁴)_(q) and q is 0.

In some embodiments, R¹ is -imidazolyl(R⁴)_(q).

In some embodiments, R¹ is -imidazol-5-yl(R⁴)_(q), q is 1, and R⁴ is Me.

In some embodiments, R¹ is -imidazol-5-yl(R⁴)_(q), q is 2, and both R⁴ are Me.

In some embodiments, R¹ is -heterocyclyl(R⁵)_(h).

In some embodiments, R¹ is -piperidinyl(R⁵)_(h).

In some embodiments, R¹ is -piperidin-4-yl(R⁵)_(h).

In some embodiments, R¹ is -piperidin-4-yl(R⁵)_(h), and h is 0.

In some embodiments, R¹ is -tetrahydropyridinyl(R⁵)_(h).

In some embodiments, R¹ is -1,2,3,6-tetrahydropyridinyl(R⁵)_(h).

In some embodiments, R¹ is -1,2,3,6-tetrahydropyridinyl(R⁵)_(h), and h is 0.

In some embodiments, R³ is H.

In some embodiments, R³ is -heteroaryl(R⁶)_(q).

In some embodiments, R³ is -heterocyclyl(R⁷)_(h).

In some embodiments, R³ is -piperidinyl(R⁷)_(h).

In some embodiments, R³ is -piperazinyl(R⁷)_(h).

In some embodiments, R³ is -morpholinyl(R⁷)_(h).

In some embodiments, R³ is -aryl(R⁸)_(k).

In some embodiments, R³ is -pyridinyl(R⁶)_(q).

In some embodiments, R³ is -pyridin-3-yl(R⁶)_(q).

In some embodiments, R³ is -pyridin-4-yl(R⁶)_(q).

In some embodiments, R³ is -pyridin-5-yl(R⁶)_(q).

In some embodiments, R³ is -pyridin-3-yl(R⁶)_(q), q is 0.

In some embodiments, R³ is -pyridin-4-yl(R⁶)_(q), q is 0.

In some embodiments, R³ is -pyridin-5-yl(R⁶)_(q), q is 0.

In some embodiments, R³ is -imidazolyl(R⁶)_(q).

In some embodiments, R³ is -imidazol-1-yl(R⁶)_(q), q is 1, and R⁶ is —(C₁₋₃ alkyl).

In some embodiments, R³ is -imidazol-1-yl(R⁶)_(q), q is 1, and R⁶ is methyl.

In some embodiments, R³ is -furanyl(R⁶)_(q).

In some embodiments, R³ is -furan-2-yl(R⁶)_(q).

In some embodiments, R³ is -furan-2-yl(R⁶)_(q) and q is 0.

In some embodiments, R³ is -furan-3-yl(R⁶)_(q).

In some embodiments, R³ is -furan-3-yl(R⁶)_(q) and q is 0.

In some embodiments, R³ is -thiophenyl(R⁶)_(q).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q) and q is 0.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1 or 2, and each R⁶ is independently a halide.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1 or 2, and R⁶ is F.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1 or 2, and each R⁶ is independently —(C₁₋₆ alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1 or 2, and each R⁶ is independently —(C₁₋₂ alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1 or 2, and R⁶ is methyl.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1 or 2, and R⁶ is —CF₃.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1 or 2, and R⁶ is CN.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1, and R⁶ is —C(═O)R¹⁷.

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C₁₋₆ alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C₁₋₄ alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C₁₋₂ alkyl).

In some embodiments, R³ is -thiophen-2-yl(R⁶)_(q), q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q) and q is 0.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1 or 2, and each R⁶ is independently halide.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1 or 2, and R⁶ is F.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1 or 2, and each R⁶ is independently —(C₁₋₆ alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1 or 2, and each R⁶ is independently —(C₁₋₂ alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1 or 2, and R⁶ is methyl.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1 or 2, and R⁶ is —CF₃.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1 or 2, and R⁶ is CN.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1, and R⁶ is —C(═O)R¹⁷.

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C₁₋₄ alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is —(C₁₋₂ alkyl).

In some embodiments, R³ is -thiophen-3-yl(R⁶)_(q), q is 1, R⁶ is —C(═O)R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is selected from the group consisting of:

In some embodiments, R³ is -phenyl(R⁸)_(k).

In some embodiments, R³ is -phenyl(R⁸)_(k) and k is 0.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 1 or 2, and each R⁸ is independently a halide.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 1 or 2, and R⁸ is F.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 1, and R⁸ is F.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is a halide and the other R⁸ is —(C₁₋₆ alkylene)_(p)NHSO₂R¹⁷.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is a halide and the other R⁸ is —(C₁₋₄ alkylene)_(p)NHSO₂R¹⁷, and p is 1.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is a halide and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷, and p is 1.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C₁₋₄ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C₁₋₂ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is a halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C₁₋₂ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C₁₋₆ alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C₁₋₅ alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C₁₋₄ alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NR¹³(C₁₋₃ alkylene)NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NR¹³CH₂CH₂NR¹³R¹⁴.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C₁₋₆ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C₁₋₄ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C₁₋₂ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is F and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from —(C₁₋₂ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is F and the other R⁸ is —NHCH₂CH₂NR¹³R¹⁴, and R¹³ and R¹⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —OCH₂CH₂NR²³R²⁴.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —OCH₂CH₂NR²³R²⁴, and R²³ and R²⁴ are independently a —(C₁₋₂ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —OCH₂CH₂NR²³R²⁴, and R²³ and R²⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is F and the other R⁸ is —OCH₂CH₂NR²³R²⁴, and R²³ and R²⁴ are both methyl.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C₁₋₄ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C₁₋₂ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is halide and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is —(C₁₋₂ alkyl).

In some embodiments, R³ is -phenyl(R⁸)_(k), k is 2, one R⁸ is F and the other R⁸ is —CH₂NHSO₂R¹⁷, and R¹⁷ is methyl.

In some embodiments, R³ is selected from the group consisting of:

In some embodiments, R³ is -piperidinyl(R⁷)_(h).

In some embodiments, R³ is -piperidin-1-yl(R⁷)_(h).

In some embodiments, R³ is -piperidin-1-yl(R⁷)_(h) and h is 0.

In some embodiments, R³ is -piperidin-1-yl(R⁷)_(h), h is 1 or 2, and each R⁷ is independently selected from a halide.

In some embodiments, R³ is -piperazinyl(R⁷)_(h).

In some embodiments, R³ is -piperazin-1-yl(R⁷)_(h).

In some embodiments, R³ is -piperazin-1-yl(R⁷)_(h), h is 1, and R⁷ is C₁₋₃ alkyl.

In some embodiments, R³ is -piperazin-1-yl(R⁷)_(h), h is 1, and R⁷ is methyl.

In some embodiments, R³ is -morpholinyl(R⁷)_(h).

In some embodiments, R³ is -morpholin-1-yl(R⁷)_(h).

In some embodiments, R³ is -morpholin-1-yl(R⁷)_(h) and h is 0.

In some embodiments, R³ is selected from the group consisting of:

In some embodiments, q is 0.

In some embodiments, at least one R⁴ is a halide.

In some embodiments, at least one R⁴ is a F.

In some embodiments, R⁴ is F.

In some embodiments, at least one R⁴ is —(C₁₋₆ alkyl).

In some embodiments, at least one R⁴ is —(C₁₋₅ alkyl).

In some embodiments, at least one R⁴ is —(C₁₋₄ alkyl).

In some embodiments, at least one R⁴ is —(C₁₋₃ alkyl).

In some embodiments, at least one R⁴ is —(C₁₋₂ alkyl).

In some embodiments, R⁴ is a methyl.

In some embodiments, at least one R⁴ is —(C₁₋₄ alkylene)_(p)heterocyclyl(R⁹)_(h) and p is 0 or 1.

In some embodiments, at least one R⁴ is —(C₁₋₃ alkylene)_(p)heterocyclyl(R⁹)_(h) and p is 0 or 1.

In some embodiments, at least one R⁴ is —(C₁₋₂ alkylene)_(p)heterocyclyl(R⁹)_(h) and p is 0 or 1.

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_(h).

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_(h) and h is 0.

In some embodiments, R⁴ is a —CH₂pyrrolidinyl(R⁹)_(h) and h is 0.

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_(h), h is 1 or 2, and at least one R⁹ is halide.

In some embodiments, at least one R⁴ is —CH₂pyrrolidinyl(R⁹)_(h), h is 1 or 2, and at least one R⁹ is F.

In some embodiments, R⁴ is a —CH₂pyrrolidinyl(R⁹)_(h), h is 1 or 2, and at least one R⁹ is halide.

In some embodiments, R⁴ is —CH₂pyrrolidinyl(R⁹)_(h), h is 1 or 2, and at least one R⁹ is F.

In some embodiments, R⁴ is a —CH₂pyrrolidinyl(R⁹)_(h), h is 1 or 2, and each R⁹ is F.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_(h).

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_(h) and h is 0.

In some embodiments, R⁴ is a —CH₂piperidinyl(R⁹)_(h) and h is 0.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_(h) and at least one R⁹ is halide.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_(h) and at least one R⁹ is F.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_(h), h is 1 or 2, and at least one R⁹ is halide.

In some embodiments, at least one R⁴ is —CH₂piperidinyl(R⁹)_(h), h is 1 or 2, and at least one R⁹ is F.

In some embodiments, R⁴ is —CH₂piperidinyl(R⁹)_(h), h is 1 or 2, and each R⁹ is a halide.

In some embodiments, R⁴ is —CH₂piperidinyl(R⁹)_(h), h is 1 or 2, and each R⁹ is F.

In some embodiments, R⁴ is a —CH₂piperidinyl(R⁹)_(h), h is 1 or 2, and each R⁹ is F.

In some embodiments, R⁴ is a

In some embodiments, at least one R⁴ is —(C₁₋₄ alkylene)_(p)carbocyclyl(R¹⁰)_(j).

In some embodiments, at least one R⁴ is —(C₁₋₄ alkylene)_(p)carbocyclyl(R¹⁰)_(j) and j is 0 or 1.

In some embodiments, at least one R⁴ is —(C₁₋₃ alkylene)_(p)carbocyclyl(R¹⁰)_(j) and j is 0 or 1.

In some embodiments, at least one R⁴ is —(C₁₋₂ alkylene)_(p)carbocyclyl(R¹⁰)_(j) and j is 0 or 1.

In some embodiments, at least one R⁴ is —CH₂carbocyclyl(R¹⁰)_(j).

In some embodiments, R⁴ is a —CH₂carbocyclyl(R¹⁰)_(j).

In some embodiments, at least one R⁴ is —(C₁₋₄ alkylene)_(p)aryl(R¹¹)_(k) and k is 0 or 1.

In some embodiments, at least one R⁴ is —(C₁₋₃ alkylene)_(p)aryl(R¹¹)_(k) and k is 0 or 1.

In some embodiments, at least one R⁴ is —(C₁₋₂ alkylene)_(p)aryl(R¹¹)_(k) and k is 0 or 1.

In some embodiments, at least one R⁴ is —CH₂aryl(R¹¹)_(k).

In some embodiments, at least one R⁴ is —CH₂phenyl(R¹¹)_(k).

In some embodiments, R⁴ is a —CH₂phenyl(R¹¹)_(k).

In some embodiments, at least one R⁴ is —NHC(═O)R¹².

In some embodiments, R⁴ is a —NHC(═O)R¹².

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₉ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₈ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₇ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₆ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₅ alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C₁₋₅ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₄ alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C₁₋₄ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₃ alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C₁₋₃ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₁₋₂ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₂₋₅ alkyl).

In some embodiments, R⁴ is a —NHC(═O)R¹² and R¹² is —(C₂₋₅ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —(C₃₋₄ alkyl).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is -aryl(R¹⁹)_(k).

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is —CH₂aryl(R¹⁹)_(k).

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is —CH₂phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, R⁴ is —NHC(═O)R¹², R¹² is —CH₂phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is -heteroaryl(R¹⁵)_(q).

In some embodiments, at least one R⁴ is —NHC(═O)R¹² and R¹² is -carbocyclyl(R²⁰)_(j).

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -carbocyclyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclopropyl(R²⁰)_(j), and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclopropyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclobutyl(R²⁰)_(j), and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclobutyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclopentyl(R²⁰)_(j), and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclopentyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is -cyclohexyl(R²⁰), and j is 0.

In some embodiments, R⁴ is a —NHC(═O)R¹², R¹² is -cyclohexyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is —CH₂carbocyclyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHC(═O)R¹², R¹² is —CH₂cyclopropyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NR¹³R¹⁴.

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C₁₋₆ alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C_(1-s) alkyl).

In some embodiments, at least one R⁴ is —NR³R⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C₁₋₄ alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C₁₋₃ alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and —(C₁₋₂ alkyl).

In some embodiments, at least one R⁴ is —NR¹³R¹⁴, and R¹³ and R¹⁴ are independently selected from the group consisting of H and methyl.

In some embodiments, at least one R⁴ is —NH₂.

In some embodiments, R⁴ is a —NH₂.

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —(C₁₋₄ alkyl).

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —(C₁₋₃ alkyl).

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —(C₁₋₂ alkyl).

In some embodiments, R⁴ is a —NHR¹⁴ and R¹⁴ is —(C₁₋₂ alkyl).

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —CH₂aryl(R¹⁹)_(k).

In some embodiments, at least one R⁴ is —NHR⁴, R¹⁴ is —CH₂phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, R⁴ is —NHR¹⁴, R¹⁴ is —CH₂phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, at least one R⁴ is —NHR¹⁴ and R¹⁴ is —CH₂carbocyclyl(R²⁰)_(j).

In some embodiments, at least one R⁴ is —NHR¹⁴, R¹⁴ is —CH₂cyclopropyl(R²⁰)_(j), and j is 0.

In some embodiments, R⁴ is a —NHR¹⁴, R¹⁴ is —CH₂cyclopropyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHR¹⁴, R¹⁴ is —CH₂cyclobutyl(R²⁰), and j is 0.

In some embodiments, R⁴ is a —NHR¹⁴, R¹⁴ is —CH₂cyclobutyl(R²⁰), and j is 0.

In some embodiments, at least one R⁴ is —NHR¹⁴, R¹⁴ is —CH₂cyclopentyl(R²⁰)_(j), and j is 0.

In some embodiments, R⁴ is a —NHR¹⁴, R¹⁴ is —CH₂cyclopentyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —NHR¹⁴, R¹⁴ is —CH₂cyclohexyl(R²⁰), and j is 0.

In some embodiments, R⁴ is a —NHR¹⁴, R¹⁴ is —CH₂cyclohexyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —(C₁₋₆ alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C₁₋₅ alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C₁₋₄ alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C₁₋₃ alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —(C₁₋₂ alkylene)NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶.

In some embodiments, R⁴ is a —CH₂NR¹⁵R¹⁶.

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C₁₋₆ alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C₁₋₅ alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C₁₋₄ alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C₁₋₃ alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and —(C₁₋₂ alkyl).

In some embodiments, at least one R⁴ is —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and methyl.

In some embodiments, R⁴ is a —CH₂NR¹⁵R¹⁶, and R¹⁵ and R¹⁶ are independently selected from the group consisting of H and methyl.

In some embodiments, at least one R⁴ is —CH₂NH₂.

In some embodiments, R⁴ is a —CH₂NH₂.

In some embodiments, at least one R⁴ is —CH₂NMe₂.

In some embodiments, R⁴ is —CH₂NMe₂.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —(C₁₋₄ alkyl).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —(C₁₋₃ alkyl).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —(C₁₋₂ alkyl).

In some embodiments, R⁴ is a —CH₂NHR¹⁶ and R¹⁶ is —(C₁₋₂ alkyl).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —CH₂aryl(R¹⁹)_(k).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂phenyl(R¹⁹)_(k), and k is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶ and R¹⁶ is —CH₂carbocyclyl(R²⁰)).

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopropyl(R²⁰)), and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopropyl(R²⁰)), and j is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclobutyl(R²⁰)_(j), and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclobutyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopentyl(R²⁰)), and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclopentyl(R²⁰)), and j is 0.

In some embodiments, at least one R⁴ is —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclohexyl(R²⁰)_(j), and j is 0.

In some embodiments, R⁴ is a —CH₂NHR¹⁶, R¹⁶ is —CH₂cyclohexyl(R²⁰), and j is 0.

In some embodiments, at least one R⁴ is —OR²².

In some embodiments, at least one R⁴ is —OH.

In some embodiments, R⁴ is a —OH.

In some embodiments, at least one R⁴ is —OR²² and R²² is —(C₁₋₃ alkyl).

In some embodiments, at least one R⁴ is —OR²² and R²² is —(C₁₋₂ alkyl).

In some embodiments, at least one R⁴ is —OMe.

In some embodiments, R⁴ is a —OMe.

In some embodiments, at least one R⁴ is —OR²², R²² is -heterocyclyl(R²¹)_(h), and h is 0.

In some embodiments, R⁴ is a —OR²², R²² is -heterocyclyl(R²¹)_(h), and h is 0.

In some embodiments, at least one R⁴ is —OR²², R²² is -carbocyclyl(R²⁰), and j is 0.

In some embodiments, R⁴ is a —OR²², R²² is -carbocyclyl(R²⁰)_(j), and j is 0.

In some embodiments, at least one R⁴ is —OR²², R²² is —(C₁₋₄ alkylene)heterocyclyl(R²¹)_(h), and h is 0.

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂CH₂)heterocyclyl(R²¹)_(h), and h is 0.

In some embodiments, R⁴ is a —OR²², R²² is —(CH₂CH₂)heterocyclyl(R²¹)_(h), and h is 0.

In some embodiments, at least one R⁴ is —OR²², R²² is —(C₁₋₄ alkylene)NR²³R²⁴ and R²³ and R²⁴ are independently a —(C₁₋₄ alkyl).

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂CH₂)NR²³R²⁴ and R²³ and R²⁴ are independently a —(C₁₋₂ alkyl).

In some embodiments, at least one R⁴ is —OR²², and R²² is —(CH₂CH₂)NMe₂.

In some embodiments, R⁴ is a —OR²², and R²² is —(CH₂CH₂)NMe₂.

In some embodiments, at least one R⁴ is —OR²², R²² is —(C₁₋₄ alkylene)aryl(R¹⁹)_(k), k is 0 or 1 and R¹⁹ is halide.

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂CH₂)phenyl(R¹⁹)_(k), k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, R⁴ is a —OR²², R²² is —(CH₂CH₂)phenyl(R¹⁹)_(k), k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, at least one R⁴ is —OR²², R²² is —(CH₂)phenyl(R¹⁹)_(k), k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, R⁴ is a —OR²², R²² is —(CH₂)phenyl(R¹⁹)_(k), k is 0 or 1 and R¹⁹ is a halide.

In some embodiments, h is 0.

In some embodiments, at least one R⁵ is a halide.

In some embodiments, at least one R⁵ is a F.

In some embodiments, at least one R⁵ is —(C₁₋₆ alkyl).

In some embodiments, at least one R⁵ is —(C₁₋₅ alkyl).

In some embodiments, at least one R⁵ is —(C₁₋₄ alkyl).

In some embodiments, at least one R⁵ is —(C₁₋₃ alkyl).

In some embodiments, at least one R⁵ is —(C₁₋₂ alkyl).

In some embodiments, at least one R⁵ is methyl.

In some embodiments, at least one R⁶ is a halide.

In some embodiments, at least one R⁶ is a F.

In some embodiments, at least one R⁶ is —(C₁₋₄ alkyl).

In some embodiments, at least one R⁶ is —(C₁₋₃ alkyl).

In some embodiments, at least one R⁶ is —(C₁₋₂ alkyl).

In some embodiments, at least one R⁶ is methyl.

In some embodiments, R⁶ is a methyl.

In some embodiments, at least one R⁶ is —C(═O)(C₁₋₃ alkyl).

In some embodiments, at least one R⁶ is —C(═O)Me.

In some embodiments, R⁶ is a —C(═O)Me.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -phenyl(R⁸)_(k); k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; and R¹⁷ is —(C₁₋₃ alkyl).

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl).

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -heterocyclyl(R⁸)_(h); h is 1 or 2; and R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl).

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_(j); j is 0; R³ is -phenyl(R⁸)_(k); k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰); j is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; R¹⁷ is —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰); j is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴; R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰); j is 0; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is C₁₋₃ alkyl; the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_(j); j is 0; R³ is -heterocyclyl(R⁷)_(h); h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰), wherein k and j are 0; R³ is -phenyl(R⁸)_(k), wherein k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰), wherein k and j are 0; R³ is -phenyl(R⁸)_(k), wherein k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; R¹⁷ is —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR′″R⁶, wherein R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl), and R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰)_(j), wherein k and j are 0; R³ is -phenyl(R⁸)_(k), wherein k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴, wherein R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰)_(j), wherein k and j are 0; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR′″R⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰), wherein k and j are 0; R³ is -heterocyclyl(R⁷)_(h); h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_(k); k is 1 or 2; R⁸ is F; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; R¹⁷ is —(C₁₋₃ alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_(k); k is 2; and R⁸ is one F and the other R⁸—NH(C₁₋₆ alkylene)NR¹³R¹⁴; R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h), wherein h is 0-2; R⁹ is F; R³ is -heterocyclyl(R⁷)_(h), wherein h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -phenyl(R⁸)_(k); k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; and R¹⁷ is —(C₁₋₃ alkyl).

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl).

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_(q), wherein q is 0; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is H; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -heterocyclyl(R⁷)_(h); h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -phenyl(R⁸)_(k); k is 1 or 2; and R⁸ is F.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; and R¹⁷ is —(C₁₋₃ alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is —(C₂₋₅ alkyl); R³ is -heterocyclyl(R⁷)_(h); h is 1 or 2; and R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl).

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R²; R¹² is -carbocyclyl(R²⁰); j is 0; R³ is -phenyl(R⁸)_(k); k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R²; R¹² is -carbocyclyl(R²⁰); j is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; R¹⁷ is —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)); j is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR³R⁴; R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is —NHC(═O)R²; R¹² is -carbocyclyl(R²⁰)_(j); j is 0; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is C₁₋₃ alkyl; the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —NHC(═O)R¹²; R¹² is -carbocyclyl(R²⁰)_(j); j is 0; R³ is -heterocyclyl(R⁷)_(h); h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰), wherein k and j are 0; R³ is -phenyl(R⁸)_(k), wherein k is 1 or 2; R⁸ is F; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰), wherein k and j are 0; R³ is -phenyl(R⁸)_(k) wherein k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; R¹⁷ is —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶, wherein R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl), and R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰)_(j), wherein k and j are 0; R³ is -phenyl(R⁸)_(k), wherein k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴, wherein R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR¹⁵R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰)_(j), wherein k and j are 0; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is selected from the group consisting of —NR¹³R¹⁴ and —CH₂NR′″R¹⁶; R¹³ and R¹⁵ are independently selected from the group consisting of H and —(C₁₋₃ alkyl); R¹⁴ and R¹⁶ are independently selected from the group consisting of H, —(C₁₋₃ alkyl), —CH₂phenyl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰)), wherein k and j are 0; R³ is -heterocyclyl(R⁷)_(h); h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl); and the carbocyclyl is selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_(k); k is 1 or 2; R⁸ is F; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R⁷; p is 1; R¹⁷ is —(C₁₋₃ alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -phenyl(R⁸)_(k); k is 2; and R⁸ is one F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴; R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q), wherein q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h); h is 0-2; R⁹ is F; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole; and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —CH₂heterocyclyl(R⁹)_(h), wherein h is 0-2; R⁹ is F; R³ is -heterocyclyl(R⁷)_(h), wherein h is 1 or 2; R⁷ is selected from the group consisting of halide and —(C₁₋₂ alkyl); and the heterocyclyl is selected from the group consisting of pyrrolidine and piperidine.

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -phenyl(R⁸)_(k); k is 1 or 2; and R⁸ is F.

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —(C₁₋₂ alkylene)_(p)NHSO₂R¹⁷; p is 1; and R¹⁷ is —(C₁₋₃ alkyl).

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -phenyl(R⁸)_(k); k is 2; one R⁸ is F and the other R⁸ is —NH(C₁₋₆ alkylene)NR¹³R¹⁴; and R¹³ and R¹⁴ are independently selected from —(C₁₋₃ alkyl).

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_(q), wherein q is 0; R³ is -heteroaryl(R⁶)_(q), wherein q is 1; R⁶ is selected from the group consisting of halide, —(C₁₋₂ alkyl), and —C(═O)R¹⁷; R¹⁷ is —(C₁₋₃ alkyl); and the heteroaryl is selected from the group consisting of pyridine, furan, thiophene, and imidazole.

In some embodiments, R² is F; R¹ is -pyrimidinyl(R⁴)_(q); q is 0; R³ is -heterocyclyl(R⁷)_(h); h is 1 or 2; R¹ is selected from the group consisting of halide and —(C₁₋₂ alkyl).

In some embodiments, R² is H; R¹ is -pyrazol-4-yl(R⁴)_(q); q is 0 or 1; R⁴ is —(C₁₋₃ alkyl); R³ is -phenyl(R⁸)_(k); k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -imidazol-5-yl(R⁴)_(q); q is 1 or 2; each R⁴ is independently selected from —(C₁₋₃ alkyl); R³ is -phenyl(R⁸)_(k); k is 1 or 2; and R⁸ is F.

In some embodiments, R² is H; R¹ is -pyridin-3-yl(R⁴)_(q); q is 1; R⁴ is —OR²²; R²² is selected from the group consisting of H and —(C₁₋₃ alkyl); R³ is -phenyl(R⁸)_(k); k is 1 or 2; and R⁸ is F.

Illustrative compounds of Formula (I) are shown in Table 1.

TABLE 1

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

49

50

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

87

88

89

90

91

92

93

94

95

96

97

98

99

100

101

102

103

104

105

106

107

108

109

110

111

112

113

114

115

116

117

118

119

120

121

122

123

124

125

126

127

128

129

130

131

132

133

134

135

136

137

138

139

140

141

142

143

144

145

146

147

148

149

150

151

152

153

154

155

156

157

158

159

160

161

162

163

164

165

166

167

168

169

170

171

172

173

174

175

176

177

178

179

180

181

182

183

184

185

186

187

188

189

190

191

192

193

194

195

196

197

198

199

200

201

202

203

204

205

206

207

208

209

210

211

212

213

214

215

216

217

218

219

220

221

222

223

224

225

226

227

228

229

230

231

232

233

234

235

236

237

238

239

240

241

242

243

244

245

246

247

248

249

250

251

252

253

254

255

256

257

258

259

260

261

262

263

264

265

266

267

268

269

270

271

272

273

274

275

276

277

278

279

280

281

282

283

284

285

286

287

288

289

290

291

292

293

294

295

296

297

298

299

300

301

302

303

304

305

306

307

308

309

310

311

312

313

314

315

316

317

318

319

320

321

322

323

324

325

326

327

328

329

330

331

332

333

334

335

336

337

338

339

340

341

342

343

344

345

346

347

348

349

350

351

352

353

354

355

356

357

358

359

360

361

362

363

364

365

366

367

368

369

370

371

372

373

374

375

376

377

378

379

380

381

382

383

384

385

386

387

388

389

390

391

392

393

394

395

396

397

398

399

400

401

402

403

404

405

406

407

408

409

410

411

412

413

414

415

416

417

418

419

420

421

422

423

424

425

426

427

428

429

430

431

432

433

434

435

436

437

438

439

440

441

442

443

444

445

446

447

448

449

450

451

452

453

454

455

456

457

458

459

460

461

462

463

464

465

466

467

468

469

470

471

472

473

474

475

476

477

478

479

480

481

482

483

484

485

486

487

488

489

490

491

492

493

494

495

496

497

498

499

500

501

502

503

504

505

506

507

508

509

510

511

512

513

514

515

516

517

518

519

520

521

522

523

524

525

526

527

528

529

530

531

532

533

534

535

536

537

538

539

540

541

542

543

544

545

546

547

548

549

550

551

552

553

554

555

556

557

558

559

560

561

562

563

564

565

566

567

568

569

570

571

572

573

574

575

576

577

578

579

580

581

582

583

584

585

586

587

588

589

590

591

592

593

594

595

596

597

598

599

600

601

602

603

604

605

606

607

608

609

610

611

612

613

614

615

616

617

618

619

620

621

622

623

624

625

626

627

628

629

630

631

632

633

634

635

636

637

638

639

640

641

642

643

644

645

646

647

648

649

650

651

652

653

654

655

656

657

658

659

660

661

662

663

664

665

666

667

668

669

670

671

672

673

674

675

676

677

678

679

680

681

682

683

684

685

686

687

688

689

690

691

692

693

694

695

696

697

698

699

700

701

702

703

704

705

706

707

708

709

710

711

712

713

714

715

716

717

718

719

720

721

722

723

724

725

726

727

728

729

730

731

732

733

734

735

736

737

738

739

740

741

742

743

744

745

746

747

748

749

750

751

752

753

754

755

756

757

758

759

760

761

762

763

764

765

766

767

768

769

770

771

772

773

774

775

776

777

778

779

780

781

782

783

784

785

786

787

788

789

790

791

792

793

794

795

796

797

798

799

800

801

802

803

804

805

806

807

808

809

810

811

812

813

814

815

816

817

818

819

820

821

822

823

824

825

826

827

828

829

830

831

832

833

834

835

836

837

838

839

840

841

842

843

844

845

846

847

848

849

850

851

852

853

854

855

856

857

858

859

860

861

862

863

864

865

866

867

868

869

870

871

872

873

874

875

876

877

878

879

880

881

882

883

884

885

886

887

888

889

890

891

892

893

894

895

896

897

898

899

900

901

902

903

904

905

906

907

908

909

910

911

912

913

914

915

916

917

918

919

920

921

922

923

924

925

926

927

928

929

930

931

932

933

934

935

936

937

938

939

940

941

942

943

944

945

946

947

948

949

950

951

952

953

954

955

956

957

958

959

960

961

962

963

964

965

966

967

968

969

970

971

972

973

974

975

976

977

978

979

980

981

982

983

984

985

986

987

988

989

990

991

992

993

994

995

996

997

998

999

1000

1001

1002

1003

1004

1005

1006

1007

1008

1009

1010

1011

1012

1013

1014

1015

1016

1017

1018

1019

1020

1021

1022

1023

1024

1025

1026

1027

1028

1029

1030

1031

1032

1033

1034

1035

1036

1037

1038

1039

1040

1041

1042

1043

1044

1045

1046

1047

1048

1049

1050

1051

1052

1053

1054

1055

1056

1057

1058

1059

1060

1061

1062

1063

1064

1065

1066

1067

1068

1069

1070

1071

1072

1073

1074

1075

1076

1077

1078

1079

1080

1081

1082

1083

1084

1085

1086

1087

1088

1089

1090

1091

1092

1093

1094

1095

1096

1097

1098

1099

1100

1101

1102

1103

1104

1105

1106

1107

1108

1109

1110

1111

1112

1113

1114

1115

1116

1117

1118

1119

1120

1121

1122

1123

1124

1125

1126

1127

1128

1129

1130

1131

1132

1133

1134

1135

1136

1137

1138

1139

1140

1141

1142

1143

1144

1145

1146

1147

1148

1149

1150

1151

1152

1153

1154

1155

1156

1157

1158

1159

1160

1161

1162

1163

1164

1165

1166

1167

1168

1169

1170

1171

1172

1173

1174

1175

1176

1177

1178

1179

1180

1181

1182

1183

1184

1185

1186

1187

1188

1189

1190

1191

1192

1193

1194

1195

1196

1197

1198

1199

1200

1201

1202

1203

1204

1205

1206

1207

1208

1209

1210

1211

1212

1213

1214

1215

1216

1217

1218

1219

1220

1221

1222

1223

1224

1225

1226

1227

1228

1229

1230

1231

1232

1233

1234

1235

1236

1237

1238

1239

1240

1241

1242

1243

1244

1245

1246

1247

1248

1249

1250

1251

1252

1253

1254

1255

1256

1257

1258

1259

1260

1261

1262

1263

1264

1265

1266

1267

1268

1269

1270

1271

1272

1273

1274

1275

1276

1277

1278

1279

1280

1281

1282

1283

1284

1285

1286

1287

1288

1289

1290

1291

1292

1293

1294

1295

1296

1297

1298

1299

1300

1301

1302

1303

1304

1305

1306

1307

1308

1309

1310

1311

1312

1313

1314

1315

1316

1317

1318

1319

1320

1321

1322

1323

1324

1325

1326

1327

1328

1329

1330

1331

1332

1333

1334

1335

1336

1337

1338

1339

1340

1341

1342

1343

1344

1345

1346

1347

1348

1349

1350

1351

1352

1353

1354

1355

1356

1357

1358

1359

1360

1361

1362

1363

1364

1365

1366

1367

1368

1369

1370

1371

1372

1373

1374

1375

1376

1377

1378

1379

1380

1381

1382

1383

1384

1385

1386

1387

1388

1389

1390

1391

1392

1393

1394

1395

1396

1397

1398

1399

1400

1401

1402

1403

1404

1405

1406

1407

1408

1409

1410

1411

1412

1413

1414

1415

1416

1417

1418

1419

1420

1421

1422

1423

1424

1425

1426

1427

1428

1429

1430

1431

1432

1433

1434

1435

1436

1437

1438

1439

1440

1441

1442

1443

1444

1445

1446

1447

1448

1449

1450

1451

1452

1453

1454

1455

1456

1457

1458

1459

1460

1461

1462

1463

1464

1465

1466

1467

1468

1469

1470

1471

1472

1473

1474

1475

1476

1477

Administration and Pharmaceutical Compositions

Some embodiments include pharmaceutical compositions comprising: (a) a therapeutically effective amount of a compound provided herein, or its corresponding enantiomer, diastereoisomer or tautomer, or pharmaceutically acceptable salt; and (b) a pharmaceutically acceptable carrier.

The compounds provided herein may also be useful in combination (administered together or sequentially) with other known agents.

Non-limiting examples of diseases which can be treated with a combination of a compound of Formula (I) and other known agents are colorectal cancer, ovarian cancer, retinitis pigmentosa, macular degeneration, diabetic retinopathy, idiopathic pulmonary fibrosis/pulmonary fibrosis, and osteoarthritis.

In some embodiments, colorectal cancer can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: 5-Fluorouracil (5-FU), which can be administered with the vitamin-like drug leucovorin (also called folinic acid); capecitabine (XELODA®), irinotecan (CAMPOSTAR®), oxaliplatin (ELOXATIN®). Examples of combinations of these drugs which could be further combined with a compound of Formula (I) are FOLFOX (5-FU, leucovorin, and oxaliplatin), FOLFIRI (5-FU, leucovorin, and irinotecan), FOLFOXIRI (leucovorin, 5-FU, oxaliplatin, and irinotecan) and CapeOx (Capecitabine and oxaliplatin). For rectal cancer, chemo with 5-FU or capecitabine combined with radiation may be given before surgery (neoadjuvant treatment).

In some embodiments, ovarian cancer can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: Topotecan, Liposomal doxorubicin (DOXIL®), Gemcitabine (GEMZAR®), Cyclophosphamide (CYTOXAN®), Vinorelbine (NAVELBINE®), Ifosfamide (IFEX®), Etoposide (VP-16), Altretamine (HEXALEN®), Capecitabine (XELODA®), Irinotecan (CPT-11, CAMPTOSAR®), Melphalan, Pemetrexed (ALIMTA®) and Albumin bound paclitaxel (nab-paclitaxel, ABRAXANE®). Examples of combinations of these drugs which could be further combined with a compound of Formula (I) are TIP (paclitaxel [Taxol], ifosfamide, and cisplatin), VeIP (vinblastine, ifosfamide, and cisplatin) and VIP (etoposide [VP-16], ifosfamide, and cisplatin).

In some embodiments, a compound of Formula (I) can be used to treat cancer in combination with any of the following methods: (a) Hormone therapy such as aromatase inhibitors, LHRH [luteinizing hormone-releasing hormone] analogs and inhibitors, and others; (b) Ablation or embolization procedures such as radiofrequency ablation (RFA), ethanol (alcohol) ablation, microwave thermotherapy and cryosurgery (cryotherapy); (c) Chemotherapy using alkylating agents such as cisplatin and carboplatin, oxaliplatin, mechlorethamine, cyclophosphamide, chlorambucil and ifosfamide; (d) Chemotherapy using anti-metabolites such as azathioprine and mercaptopurine; (e) Chemotherapy using plant alkaloids and terpenoids such as vinca alkaloids (i.e. Vincristine, Vinblastine, Vinorelbine and Vindesine) and taxanes; (f) Chemotherapy using podophyllotoxin, etoposide, teniposide and docetaxel; (g) Chemotherapy using topoisomerase inhibitors such as irinotecan, topotecan, amsacrine, etoposide, etoposide phosphate, and teniposide; (h) Chemotherapy using cytotoxic antibiotics such as actinomycin, anthracyclines, doxorubicin, daunorubicin, valrubicin, idarubicin, epirubicin, bleomycin, plicamycin and mitomycin; (i) Chemotherapy using tyrosine-kinase inhibitors such as Imatinib mesylate (GLEEVEC®, also known as STI-571), Gefitinib (Iressa, also known as ZD1839), Erlotinib (marketed as TARCEVA®), Bortezomib (VELCADE®), tamoxifen, tofacitinib, crizotinib, Bcl-2 inhibitors (e.g. obatoclax in clinical trials, ABT-263, and Gossypol), PARP inhibitors (e.g. Iniparib, Olaparib in clinical trials), PI3K inhibitors (eg. perifosine in a phase III trial), VEGF Receptor 2 inhibitors (e.g. Apatinib), AN-152, (AEZS-108), Braf inhibitors (e.g. vemurafenib, dabrafenib and LGX818), MEK inhibitors (e.g. trametinib and MEK162), CDK inhibitors, (e.g. PD-0332991), salinomycin and Sorafenib; (j) Chemotherapy using monoclonal antibodies such as Rituximab (marketed as MABTHERA® or RITUXAN®), Trastuzumab (Herceptin also known as ErbB2), Cetuximab (marketed as ERBITUX®), and Bevacizumab (marketed as AVASTIN®); and (k) radiation therapy.

In some embodiments, diabetic retinopathy can be treated with a combination of a compound of Formula (I) and one or more of the following natural supplements: Bilberry, Butcher's broom, Ginkgo, Grape seed extract, and Pycnogenol (Pine bark).

In some embodiments, idiopathic pulmonary fibrosis/pulmonary fibrosis can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: pirfenidone (pirfenidone was approved for use in 2011 in Europe under the brand name Esbriet®), prednisone, azathioprine, N-acetylcysteine, interferon-γ 1b, bosentan (bosentan is currently being studied in patients with IPF, [The American Journal of Respiratory and Critical Care Medicine (2011), 184(1), 92-9]), Nintedanib (BIBF 1120 and Vargatef), QAX576 [British Journal of Pharmacology (2011), 163(1), 141-172], and anti-inflammatory agents such as corticosteroids.

In some embodiments, a compound of Formula (I) can be used to treat idiopathic pulmonary fibrosis/pulmonary fibrosis in combination with any of the following methods: oxygen therapy, pulmonary rehabilitation and surgery.

In some embodiments, a compound of Formula (I) can be used to treat osteoarthritis in combination with any of the following methods: (a) Nonsteroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen, naproxen, aspirin and acetaminophen; (b) physical therapy; (c) injections of corticosteroid medications; (d) injections of hyaluronic acid derivatives (e.g. Hyalgan, Synvisc); (e) narcotics, like codeine; (f) in combination with braces and/or shoe inserts or any device that can immobilize or support your joint to help you keep pressure off it (e.g., splints, braces, shoe inserts or other medical devices); (g) realigning bones (osteotomy); (h) joint replacement (arthroplasty); and (i) in combination with a chronic pain class.

In some embodiments, macular degeneration can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: Bevacizumab (Avastin®), Ranibizumab (Lucentis®), Pegaptanib (Macugen), Aflibercept (Eylea®), verteporfin (Visudyne®) in combination with photodynamic therapy (PDT) or with any of the following methods: (a) in combination with laser to destroy abnormal blood vessels (photocoagulation); and (b) in combination with increased vitamin intake of antioxidant vitamins and zinc.

In some embodiments, retinitis pigmentosa can be treated with a combination of a compound of Formula (I) and one or more of the following drugs: UF-021 (Ocuseva™), vitamin A palmitate and pikachurin or with any of the following methods: (a) with the Argus® II retinal implant; and (b) with stem cell and/or gene therapy.

Administration of the compounds disclosed herein or the pharmaceutically acceptable salts thereof can be via any of the accepted modes of administration, including, but not limited to, orally, subcutaneously, intravenously, intranasally, topically, transdermally, intraperitoneally, intramuscularly, intrapulmonarilly, vaginally, rectally, ontologically, neuro-otologically, intraocularly, subconjuctivally, via anterior eye chamber injection, intravitreally, intraperitoneally, intrathecally, intracystically, intrapleurally, via wound irrigation, intrabuccally, intra-abdominally, intra-articularly, intra-aurally, intrabronchially, intracapsularly, intrameningeally, via inhalation, via endotracheal or endobronchial instillation, via direct instillation into pulmonary cavities, intraspinally, intrasynovially, intrathoracically, via thoracostomy irrigation, epidurally, intratympanically, intracisternally, intravascularly, intraventricularly, intraosseously, via irrigation of infected bone, or via application as part of any admixture with a prosthetic devices. In some embodiments, the administration method includes oral or parenteral administration.

Compounds provided herein intended for pharmaceutical use may be administered as crystalline or amorphous products. Pharmaceutically acceptable compositions may include solid, semi-solid, liquid, solutions, colloidal, liposomes, emulsions, suspensions, complexes, coacervates and aerosols. Dosage forms, such as, e.g., tablets, capsules, powders, liquids, suspensions, suppositories, aerosols, implants, controlled release or the like. They may be obtained, for example, as solid plugs, powders, or films by methods such as precipitation, crystallization, milling, grinding, supercritical fluid processing, coacervation, complex coacervation, encapsulation, emulsification, complexation, freeze drying, spray drying, or evaporative drying. Microwave or radio frequency drying may be used for this purpose. The compounds can also be administered in sustained or controlled release dosage forms, including depot injections, osmotic pumps, pills (tablets and or capsules), transdermal (including electrotransport) patches, implants and the like, for prolonged and/or timed, pulsed administration at a predetermined rate.

The compounds can be administered either alone or in combination with a conventional pharmaceutical carrier, excipient or the like. Pharmaceutically acceptable excipients include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, self-emulsifying drug delivery systems (SEDDS) such as d-α-tocopherol polyethylene glycol 1000 succinate, surfactants used in pharmaceutical dosage forms such as Tweens, poloxamers or other similar polymeric delivery matrices, serum proteins, such as human serum albumin, buffer substances such as phosphates, tris, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium-chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethyl cellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, and wool fat. Cyclodextrins such as α-, β, and γ-cyclodextrin, or chemically modified derivatives such as hydroxyalkylcyclodextrins, including 2- and 3-hydroxypropyl-β-cyclodextrins, or other solubilized derivatives can also be used to enhance delivery of compounds described herein. Dosage forms or compositions containing a compound as described herein in the range of 0.005% to 100% with the balance made up from non-toxic carrier may be prepared. The contemplated compositions may contain 0.001%-100% of a compound provided herein, in one embodiment 0.1-95%, in another embodiment 75-85%, in a further embodiment 20-80%. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington: The Science and Practice of Pharmacy, 22^(nd) Edition (Pharmaceutical Press, London, U K. 2012).

In one embodiment, the compositions will take the form of a unit dosage form such as a pill or tablet and thus the composition may contain, along with a compound provided herein, a diluent such as lactose, sucrose, dicalcium phosphate, or the like; a lubricant such as magnesium stearate or the like; and a binder such as starch, gum acacia, polyvinylpyrrolidine, gelatin, cellulose, cellulose derivatives or the like. In another solid dosage form, a powder, marume, solution or suspension (e.g., in propylene carbonate, vegetable oils, PEG's, poloxamer 124 or triglycerides) is encapsulated in a capsule (gelatin or cellulose base capsule). Unit dosage forms in which one or more compounds provided herein or additional active agents are physically separated are also contemplated; e.g., capsules with granules (or tablets in a capsule) of each drug; two-layer tablets; two-compartment gel caps, etc. Enteric coated or delayed release oral dosage forms are also contemplated.

Liquid pharmaceutically administrable compositions can, for example, be prepared by dissolving, dispersing, etc. a compound provided herein and optional pharmaceutical adjuvants in a carrier (e.g., water, saline, aqueous dextrose, glycerol, glycols, ethanol or the like) to form a solution, colloid, liposome, emulsion, complexes, coacervate or suspension. If desired, the pharmaceutical composition can also contain minor amounts of nontoxic auxiliary substances such as wetting agents, emulsifying agents, co-solvents, solubilizing agents, pH buffering agents and the like (e.g., sodium acetate, sodium citrate, cyclodextrin derivatives, sorbitan monolaurate, triethanolamine acetate, triethanolamine oleate, and the like).

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.25 mg/Kg to about 50 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.25 mg/Kg to about 20 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.50 mg/Kg to about 19 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 0.75 mg/Kg to about 18 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.0 mg/Kg to about 17 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.25 mg/Kg to about 16 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.50 mg/Kg to about 15 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 1.75 mg/Kg to about 14 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 2.0 mg/Kg to about 13 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 3.0 mg/Kg to about 12 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 4.0 mg/Kg to about 11 mg/Kg in humans.

In some embodiments, the unit dosage of compounds of Formula (I) is about 5.0 mg/Kg to about 10 mg/Kg in humans.

In some embodiments, the compositions are provided in unit dosage forms suitable for single administration.

In some embodiments, the compositions are provided in unit dosage forms suitable for twice a day administration.

In some embodiments, the compositions are provided in unit dosage forms suitable for three times a day administration.

Injectables can be prepared in conventional forms, either as liquid solutions, colloid, liposomes, complexes, coacervate or suspensions, as emulsions, or in solid forms suitable for reconstitution in liquid prior to injection. The percentage of a compound provided herein contained in such parenteral compositions is highly dependent on the specific nature thereof, as well as the activity of the compound and the needs of the patient. However, percentages of active ingredient of 0.01% to 10% in solution are employable, and could be higher if the composition is a solid or suspension, which could be subsequently diluted to the above percentages.

In some embodiments, the composition will comprise about 0.1-10% of the active agent in solution.

In some embodiments, the composition will comprise about 0.1-5% of the active agent in solution.

In some embodiments, the composition will comprise about 0.1-4% of the active agent in solution.

In some embodiments, the composition will comprise about 0.15-3% of the active agent in solution.

In some embodiments, the composition will comprise about 0.2-2% of the active agent in solution.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-96 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-72 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-48 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-24 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-12 hours.

In some embodiments, the compositions are provided in dosage forms suitable for continuous dosage by intravenous infusion over a period of about 1-6 hours.

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 5 mg/m² to about 300 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 5 mg/m² to about 200 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 5 mg/m² to about 100 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 10 mg/m² to about 50 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 50 mg/m² to about 200 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 75 mg/m² to about 175 mg/m².

In some embodiments, these compositions can be administered by intravenous infusion to humans at doses of about 100 mg/m² to about 150 mg/m².

It is to be noted that concentrations and dosage values may also vary depending on the specific compound and the severity of the condition to be alleviated. It is to be further understood that for any particular patient, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that the concentration ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed compositions.

In one embodiment, the compositions can be administered to the respiratory tract (including nasal and pulmonary) e.g., through a nebulizer, metered-dose inhalers, atomizer, mister, aerosol, dry powder inhaler, insufflator, liquid instillation or other suitable device or technique.

In some embodiments, aerosols intended for delivery to the nasal mucosa are provided for inhalation through the nose. For optimal delivery to the nasal cavities, inhaled particle sizes of about 5 to about 100 microns are useful, with particle sizes of about 10 to about 60 microns being preferred. For nasal delivery, a larger inhaled particle size may be desired to maximize impaction on the nasal mucosa and to minimize or prevent pulmonary deposition of the administered formulation. In some embodiments, aerosols intended for delivery to the lung are provided for inhalation through the nose or the mouth. For delivery to the lung, inhaled aerodynamic particle sizes of about less than 10 μm are useful (e.g., about 1 to about 10 microns). Inhaled particles may be defined as liquid droplets containing dissolved drug, liquid droplets containing suspended drug particles (in cases where the drug is insoluble in the suspending medium), dry particles of pure drug substance, drug substance incorporated with excipients, liposomes, emulsions, colloidal systems, coacervates, aggregates of drug nanoparticles, or dry particles of a diluent which contain embedded drug nanoparticles.

In some embodiments, compounds of Formula (I) disclosed herein intended for respiratory delivery (either systemic or local) can be administered as aqueous formulations, as non-aqueous solutions or suspensions, as suspensions or solutions in halogenated hydrocarbon propellants with or without alcohol, as a colloidal system, as emulsions, coacervates, or as dry powders. Aqueous formulations may be aerosolized by liquid nebulizers employing either hydraulic or ultrasonic atomization or by modified micropump systems (like the soft mist inhalers, the Aerodose® or the AERx® systems). Propellant-based systems may use suitable pressurized metered-dose inhalers (pMDIs). Dry powders may use dry powder inhaler devices (DPIs), which are capable of dispersing the drug substance effectively. A desired particle size and distribution may be obtained by choosing an appropriate device.

In some embodiments, the compositions of Formula (I) disclosed herein can be administered to the ear by various methods. For example, a round window catheter (e.g., U.S. Pat. Nos. 6,440,102 and 6,648,873) can be used.

Alternatively, formulations can be incorporated into a wick for use between the outer and middle ear (e.g., U.S. Pat. No. 6,120,484) or absorbed to collagen sponge or other solid support (e.g., U.S. Pat. No. 4,164,559).

If desired, formulations of the invention can be incorporated into a gel formulation (e.g., U.S. Pat. Nos. 4,474,752 and 6,911,211).

In some embodiments, compounds of Formula (I) disclosed herein intended for delivery to the ear can be administered via an implanted pump and delivery system through a needle directly into the middle or inner ear (cochlea) or through a cochlear implant stylet electrode channel or alternative prepared drug delivery channel such as but not limited to a needle through temporal bone into the cochlea.

Other options include delivery via a pump through a thin film coated onto a multichannel electrode or electrode with a specially imbedded drug delivery channel (pathways) carved into the thin film for this purpose. In other embodiments the acidic or basic solid compound of Formula (I) can be delivered from the reservoir of an external or internal implanted pumping system.

Formulations of the invention also can be administered to the ear by intratympanic injection into the middle ear, inner ear, or cochlea (e.g., U.S. Pat. No. 6,377,849 and Ser. No. 11/337,815).

Intratympanic injection of therapeutic agents is the technique of injecting a therapeutic agent behind the tympanic membrane into the middle and/or inner ear. In one embodiment, the formulations described herein are administered directly onto the round window membrane via transtympanic injection. In another embodiment, the ion channel modulating agent auris-acceptable formulations described herein are administered onto the round window membrane via a non-transtympanic approach to the inner ear. In additional embodiments, the formulation described herein is administered onto the round window membrane via a surgical approach to the round window membrane comprising modification of the crista fenestrae cochleae.

In some embodiments, the compounds of Formula (I) are formulated in rectal compositions such as enemas, rectal gels, rectal foams, rectal aerosols, suppositories, jelly suppositories, or retention enemas, containing conventional suppository bases such as cocoa butter or other glycerides, as well as synthetic polymers such as polyvinylpyrrolidone, PEG (like PEG ointments), and the like.

Suppositories for rectal administration of the drug (either as a solution, colloid, suspension or a complex) can be prepared by mixing a compound provided herein with a suitable non-irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt or erode/dissolve in the rectum and release the compound. Such materials include cocoa butter, glycerinated gelatin, hydrogenated vegetable oils, poloxamers, mixtures of polyethylene glycols of various molecular weights and fatty acid esters of polyethylene glycol. In suppository forms of the compositions, a low-melting wax such as, but not limited to, a mixture of fatty acid glycerides, optionally in combination with cocoa butter, is first melted.

Solid compositions can be provided in various different types of dosage forms, depending on the physicochemical properties of the compound provided herein, the desired dissolution rate, cost considerations, and other criteria. In one of the embodiments, the solid composition is a single unit. This implies that one unit dose of the compound is comprised in a single, physically shaped solid form or article. In other words, the solid composition is coherent, which is in contrast to a multiple unit dosage form, in which the units are incoherent.

Examples of single units which may be used as dosage forms for the solid composition include tablets, such as compressed tablets, film-like units, foil-like units, wafers, lyophilized matrix units, and the like. In one embodiment, the solid composition is a highly porous lyophilized form. Such lyophilizates, sometimes also called wafers or lyophilized tablets, are particularly useful for their rapid disintegration, which also enables the rapid dissolution of the compound.

On the other hand, for some applications the solid composition may also be formed as a multiple unit dosage form as defined above. Examples of multiple units are powders, granules, microparticles, pellets, mini-tablets, beads, lyophilized powders, and the like. In one embodiment, the solid composition is a lyophilized powder. Such a dispersed lyophilized system comprises a multitude of powder particles, and due to the lyophilization process used in the formation of the powder, each particle has an irregular, porous microstructure through which the powder is capable of absorbing water very rapidly, resulting in quick dissolution. Effervescent compositions are also contemplated to aid the quick dispersion and absorption of the compound.

Another type of multiparticulate system which is also capable of achieving rapid drug dissolution is that of powders, granules, or pellets from water-soluble excipients which are coated with a compound provided herein so that the compound is located at the outer surface of the individual particles. In this type of system, the water-soluble low molecular weight excipient may be useful for preparing the cores of such coated particles, which can be subsequently coated with a coating composition comprising the compound and, for example, one or more additional excipients, such as a binder, a pore former, a saccharide, a sugar alcohol, a film-forming polymer, a plasticizer, or other excipients used in pharmaceutical coating compositions.

Also provided herein are kits. Typically, a kit includes one or more compounds or compositions as described herein. In certain embodiments, a kit can include one or more delivery systems, e.g., for delivering or administering a compound as provided herein, and directions for use of the kit (e.g., instructions for treating a patient). In another embodiment, the kit can include a compound or composition as described herein and a label that indicates that the contents are to be administered to a patient with cancer. In another embodiment, the kit can include a compound or composition as described herein and a label that indicates that the contents are to be administered to a patient with one or more of hepatocellular carcinoma, colon cancer, leukemia, lymphoma, sarcoma, ovarian cancer, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, sepsis, ankylosing spondylitis, psoriasis, scleroderma, mycotic and viral infections, bone and cartilage diseases, Alzheimer's disease, lung disease, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, articular cartilage (chondral) defects, degenerative disc disease (or intervertebral disc degeneration), polyposis coli, bone density and vascular defects in the eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye diseases or syndromes associated with defects and/or damaged photoreceptors, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia, Müllerian-duct regression and virilization, SERKAL syndrome, type II diabetes, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease, and Rett syndrome.

Methods of Treatment

The compounds and compositions provided herein can be used as inhibitors and/or modulators of one or more components of the Wnt pathway, which may include one or more Wnt proteins, and thus can be used to treat a variety of disorders and diseases in which aberrant Wnt signaling is implicated, such as cancer and other diseases associated with abnormal angiogenesis, cellular proliferation, and cell cycling. Accordingly, the compounds and compositions provided herein can be used to treat cancer, to reduce or inhibit angiogenesis, to reduce or inhibit cellular proliferation, to correct a genetic disorder, and/or to treat a neurological condition/disorder/disease due to mutations or dysregulation of the Wnt pathway and/or of one or more of Wnt signaling components. Non-limiting examples of diseases which can be treated with the compounds and compositions provided herein include a variety of cancers, diabetic retinopathy, pulmonary fibrosis, rheumatoid arthritis, scleroderma, mycotic and viral infections, bone and cartilage diseases, neurological conditions/diseases such as Alzheimer's disease, amyotrophic lateral sclerosis (ALS), motor neuron disease, multiple sclerosis or autism, lung disease, bone/osteoporotic (wrist, spine, shoulder and hip) fractures, polyposis coli, bone density and vascular defects in the eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye diseases or syndromes associated with defects and/or damaged photoreceptors, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia, Müllerian-duct regression and virilization, SERKAL syndrome, type II diabetes, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease and Rett syndrome.

In some embodiments, non-limiting examples of eye diseases which can be treated with the compounds and compositions provided herein include age-related macular degeneration (AMD or ARMD), rod cone dystrophy, retinitis pigmentosa (RP), acute idiopathic blind spot enlargement (AIBSE), acute zonal occult outer retinopathy (AZOOR), acute macular neuroretinopathy (AMN), multiple evanescent white dot syndrome (MEWDS), multifocal choroiditis, opticneuropathy. Further causes of photoreceptor damage that can be treated with the compounds and compositions provided herein include retinal detachment, vascular disturbance, eye tumors or extreme light damage.

With respect to cancer, the Wnt pathway is known to be constitutively activated in a variety of cancers including, for example, colon cancer, hepatocellular carcinoma, lung cancer, ovarian cancer, prostate cancer, pancreatic cancer and leukemias such as CML, CLL and T-ALL. Accordingly, the compounds and compositions described herein may be used to treat these cancers in which the Wnt pathway is constitutively activated. In certain embodiments, the cancer is chosen from hepatocellular carcinoma, colon cancer, leukemia, lymphoma, sarcoma and ovarian cancer.

Other cancers can also be treated with the compounds and compositions described herein.

More particularly, cancers that may be treated by the compounds, compositions and methods described herein include, but are not limited to, the following:

1) Breast cancers, including, for example ER⁺breast cancer, ER⁻ breast cancer, her2⁻ breast cancer, her2⁺ breast cancer, stromal tumors such as fibroadenomas, phyllodes tumors, and sarcomas, and epithelial tumors such as large duct papillomas; carcinomas of the breast including in situ (noninvasive) carcinoma that includes ductal carcinoma in situ (including Paget's disease) and lobular carcinoma in situ, and invasive (infiltrating) carcinoma including, but not limited to, invasive ductal carcinoma, invasive lobular carcinoma, medullary carcinoma, colloid (mucinous) carcinoma, tubular carcinoma, and invasive papillary carcinoma; and miscellaneous malignant neoplasms. Further examples of breast cancers can include luminal A, luminal B, basal A, basal B, and triple negative breast cancer, which is estrogen receptor negative (ER⁻), progesterone receptor negative, and her2 negative (her2⁻). In some embodiments, the breast cancer may have a high risk Oncotype score.

2) Cardiac cancers, including, for example sarcoma, e.g., angiosarcoma, fibrosarcoma, rhabdomyosarcoma, and liposarcoma; myxoma; rhabdomyoma; fibroma; lipoma and teratoma.

3) Lung cancers, including, for example, bronchogenic carcinoma, e.g., squamous cell, undifferentiated small cell, undifferentiated large cell, and adenocarcinoma; alveolar and bronchiolar carcinoma; bronchial adenoma; sarcoma; lymphoma; chondromatous hamartoma; and mesothelioma.

4) Gastrointestinal cancer, including, for example, cancers of the esophagus, e.g., squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, and lymphoma; cancers of the stomach, e.g., carcinoma, lymphoma, and leiomyosarcoma; cancers of the pancreas, e.g., ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, and vipoma; cancers of the small bowel, e.g., adenocarcinoma, lymphoma, carcinoid tumors, Kaposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, and fibroma; cancers of the large bowel, e.g., adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, and leiomyoma.

5) Genitourinary tract cancers, including, for example, cancers of the kidney, e.g., adenocarcinoma, Wilm's tumor (nephroblastoma), lymphoma, and leukemia; cancers of the bladder and urethra, e.g., squamous cell carcinoma, transitional cell carcinoma, and adenocarcinoma; cancers of the prostate, e.g., adenocarcinoma, and sarcoma; cancer of the testis, e.g., seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, and lipoma.

6) Liver cancers, including, for example, hepatoma, e.g., hepatocellular carcinoma; cholangiocarcinoma; hepatoblastoma; angiosarcoma; hepatocellular adenoma; and hemangioma.

7) Bone cancers, including, for example, osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochrondroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofibroma, osteoid osteoma and giant cell tumors.

8) Nervous system cancers, including, for example, cancers of the skull, e.g., osteoma, hemangioma, granuloma, xanthoma, and osteitis deformans; cancers of the meninges, e.g., meningioma, meningiosarcoma, and gliomatosis; cancers of the brain, e.g., astrocytoma, medulloblastoma, glioma, ependymoma, germinoma (pinealoma), glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, and congenital tumors; and cancers of the spinal cord, e.g., neurofibroma, meningioma, glioma, and sarcoma.

9) Gynecological cancers, including, for example, cancers of the uterus, e.g., endometrial carcinoma; cancers of the cervix, e.g., cervical carcinoma, and pre tumor cervical dysplasia; cancers of the ovaries, e.g., ovarian carcinoma, including serous cystadenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma, granulosa theca cell tumors, Sertoli Leydig cell tumors, dysgerminoma, and malignant teratoma; cancers of the vulva, e.g., squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, and melanoma; cancers of the vagina, e.g., clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma, and embryonal rhabdomyosarcoma; and cancers of the fallopian tubes, e.g., carcinoma.

10) Hematologic cancers, including, for example, cancers of the blood, e.g., acute myeloid leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia, chronic lymphocytic leukemia, myeloproliferative diseases, multiple myeloma, and myelodysplastic syndrome, Hodgkin's lymphoma, non-Hodgkin's lymphoma (malignant lymphoma) and Waldenström's macroglobulinemia.

11) Skin cancers and skin disorders, including, for example, malignant melanoma and metastatic melanoma, basal cell carcinoma, squamous cell carcinoma, Kaposi's sarcoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, keloids, and scleroderma.

12) Adrenal gland cancers, including, for example, neuroblastoma.

Cancers may be solid tumors that may or may not be metastatic. Cancers may also occur, as in leukemia, as a diffuse tissue. Thus, the term “tumor cell,” as provided herein, includes a cell afflicted by any one of the above identified disorders.

A method of treating cancer using a compound or composition as described herein may be combined with existing methods of treating cancers, for example by chemotherapy, irradiation, or surgery (e.g., oophorectomy). In some embodiments, a compound or composition can be administered before, during, or after another anticancer agent or treatment.

The compounds and compositions described herein can be used as anti-angiogenesis agents and as agents for modulating and/or inhibiting the activity of protein kinases, thus providing treatments for cancer and other diseases associated with cellular proliferation mediated by protein kinases. For example, the compounds described herein can inhibit the activity of one or more kinases. Accordingly, provided herein is a method of treating cancer or preventing or reducing angiogenesis through kinase inhibition.

In addition, and including treatment of cancer, the compounds and compositions described herein can function as cell-cycle control agents for treating proliferative disorders in a patient. Disorders associated with excessive proliferation include, for example, cancers, scleroderma, immunological disorders involving undesired proliferation of leukocytes, and restenosis and other smooth muscle disorders. Furthermore, such compounds may be used to prevent de-differentiation of post-mitotic tissue and/or cells.

Diseases or disorders associated with uncontrolled or abnormal cellular proliferation include, but are not limited to, the following:

-   -   a variety of cancers, including, but not limited to, carcinoma,         hematopoietic tumors of lymphoid lineage, hematopoietic tumors         of myeloid lineage, tumors of mesenchymal origin, tumors of the         central and peripheral nervous system and other tumors including         melanoma, seminoma and Kaposi's sarcoma.     -   a disease process which features abnormal cellular         proliferation, e.g., benign prostatic hyperplasia, familial         adenomatosis polyposis, neurofibromatosis, atherosclerosis,         arthritis, glomerulonephritis, restenosis following angioplasty         or vascular surgery, inflammatory bowel disease, transplantation         rejection, endotoxic shock, and fungal infections. Fibrotic         disorders such as skin fibrosis; scleroderma; progressive         systemic fibrosis; lung fibrosis; muscle fibrosis; kidney         fibrosis; glomerulosclerosis; glomerulonephritis; hypertrophic         scar formation; uterine fibrosis; renal fibrosis; cirrhosis of         the liver, liver fibrosis; fatty liver disease (FLD); adhesions,         such as those occurring in the abdomen, pelvis, spine or         tendons; chronic obstructive pulmonary disease; fibrosis         following myocardial infarction; pulmonary fibrosis; fibrosis         and scarring associated with diffuse/interstitial lung disease;         central nervous system fibrosis, such as fibrosis following         stroke; fibrosis associated with neuro-degenerative disorders         such as Alzheimer's Disease or multiple sclerosis; fibrosis         associated with proliferative vitreoretinopathy (PVR);         restenosis; endometriosis; ischemic disease and radiation         fibrosis.     -   defective apoptosis-associated conditions, such as cancers         (including but not limited to those types mentioned herein),         viral infections (including but not limited to herpesvirus,         poxvirus, Epstein-Barr virus, Sindbis virus and adenovirus),         prevention of AIDS development in HIV-infected individuals,         autoimmune diseases (including but not limited to systemic lupus         erythematosus, rheumatoid arthritis, sepsis, ankylosing         spondylitis, psoriasis, scleroderma, autoimmune mediated         glomerulonephritis, inflammatory bowel disease and autoimmune         diabetes mellitus), neuro-degenerative disorders (including but         not limited to Alzheimer's disease, lung disease, amyotrophic         lateral sclerosis, retinitis pigmentosa, Parkinson's disease,         AIDS-related dementia, spinal muscular atrophy and cerebellar         degeneration), myelodysplastic syndromes, aplastic anemia,         ischemic injury associated with myocardial infarctions, stroke         and reperfusion injury, arrhythmia, atherosclerosis,         toxin-induced or alcohol related liver diseases, hematological         diseases (including but not limited to chronic anemia and         aplastic anemia), degenerative diseases of the musculoskeletal         system (including but not limited to osteoporosis and         arthritis), tendinopathies such as tendinitis and tendinosis,         aspirin-sensitive rhinosinusitis, cystic fibrosis, multiple         sclerosis, kidney diseases and cancer pain.     -   genetic diseases due to mutations in Wnt signaling components,         such as polyposis coli, bone density and vascular defects in the         eye (Osteoporosis-pseudoglioma Syndrome, OPPG) and other eye         diseases or syndromes associated with defects and/or damaged         photoreceptors, familial exudative vitreoretinopathy, retinal         angiogenesis, early coronary disease, tetra-amelia,         Müllerian-duct regression and virilization, SERKAL syndrome,         type II diabetes, Fuhrmann syndrome,         Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome,         odonto-onycho-dermal dysplasia, obesity, split-hand/foot         malformation, caudal duplication, tooth agenesis, Wilms tumor,         skeletal dysplasia, focal dermal hypoplasia, autosomal recessive         anonychia, neural tube defects, alpha-thalassemia (ATRX)         syndrome, fragile X syndrome, ICF syndrome, Angelman's syndrome,         Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie         disease and Rett syndrome.

The compounds and compositions described herein can be used to treat neurological conditions, disorders and/or diseases caused by dysfunction in the Wnt signaling pathway. Non-limiting examples of neurological conditions/disorders/diseases which can be treated with the compounds and compositions provided herein include Alzheimer's disease, aphasia, apraxia, arachnoiditis, ataxia telangiectasia, attention deficit hyperactivity disorder, auditory processing disorder, autism, alcoholism, Bell's palsy, bipolar disorder, brachial plexus injury, Canavan disease, carpal tunnel syndrome, causalgia, central pain syndrome, central pontine myelinolysis, centronuclear myopathy, cephalic disorder, cerebral aneurysm, cerebral arteriosclerosis, cerebral atrophy, cerebral gigantism, cerebral palsy, cerebral vasculitis, cervical spinal stenosis, Charcot-Marie-Tooth disease, Chiari malformation, chronic fatigue syndrome, chronic inflammatory demyelinating polyneuropathy (CIDP), chronic pain, Coffin-Lowry syndrome, complex regional pain syndrome, compression neuropathy, congenital facial diplegia, corticobasal degeneration, cranial arteritis, craniosynostosis, Creutzfeldt-Jakob disease, cumulative trauma disorder, Cushing's syndrome, cytomegalic inclusion body disease (CIBD), Dandy-Walker syndrome, Dawson disease, de Morsier's syndrome, Dejerine-Klumpke palsy, Dejerine-Sottas disease, delayed sleep phase syndrome, dementia, dermatomyositis, developmental dyspraxia, diabetic neuropathy, diffuse sclerosis, Dravet syndrome, dysautonomia, dyscalculia, dysgraphia, dyslexia, dystonia, empty sella syndrome, encephalitis, encephalocele, encephalotrigeminal angiomatosis, encopresis, epilepsy, Erb's palsy, erythromelalgia, essential tremor, Fabry's disease, Fahr's syndrome, familial spastic paralysis, febrile seizure, Fisher syndrome, Friedreich's ataxia, fibromyalgia, Foville's syndrome, Gaucher's disease, Gerstmann's syndrome, giant cell arteritis, giant cell inclusion disease, globoid cell leukodystrophy, gray matter heterotopia, Guillain-Barré syndrome, HTLV-1 associated myelopathy, Hallervorden-Spatz disease, hemifacial spasm, hereditary spastic paraplegia, heredopathia atactica polyneuritiformis, herpes zoster oticus, herpes zoster, Hirayama syndrome, holoprosencephaly, Huntington's disease, hydranencephaly, hydrocephalus, hypercortisolism, hypoxia, immune-mediated encephalomyelitis, inclusion body myositis, incontinentia pigmenti, infantile phytanic acid storage disease, infantile Refsum disease, infantile spasms, inflammatory myopathy, intracranial cyst, intracranial hypertension, Joubert syndrome, Karak syndrome, Kearns-Sayre syndrome, Kennedy disease, Kinsbourne syndrome, Klippel Feil syndrome, Krabbe disease, Kugelberg-Welander disease, kuru, Lafora disease, Lambert-Eaton myasthenic syndrome, Landau-Kleffner syndrome, lateral medullary (Wallenberg) syndrome, Leigh's disease, Lennox-Gastaut syndrome, Lesch-Nyhan syndrome, leukodystrophy, Lewy body dementia, lissencephaly, locked-in syndrome, Lou Gehrig's disease, lumbar disc disease, lumbar spinal stenosis, Lyme disease, Machado-Joseph disease (Spinocerebellar ataxia type 3), macrencephaly, macropsia, megalencephaly, Melkersson-Rosenthal syndrome, Meniere's disease, meningitis, Menkes disease, metachromatic leukodystrophy, microcephaly, micropsia, Miller Fisher syndrome, misophonia, mitochondrial myopathy, Mobius syndrome, monomelic amyotrophy, motor neuron disease, motor skills disorder, Moyamoya disease, mucopolysaccharidoses, multi-infarct dementia, multifocal motor neuropathy, multiple sclerosis, multiple system atrophy, muscular dystrophy, myalgic encephalomyelitis, myasthenia gravis, myelinoclastic diffuse sclerosis, myoclonic Encephalopathy of infants, myoclonus, myopathy, myotubular myopathy, myotonia congenital, narcolepsy, neurofibromatosis, neuroleptic malignant syndrome, lupus erythematosus, neuromyotonia, neuronal ceroid lipofuscinosis, Niemann-Pick disease, O'Sullivan-McLeod syndrome, occipital Neuralgia, occult Spinal Dysraphism Sequence, Ohtahara syndrome, olivopontocerebellar atrophy, opsoclonus myoclonus syndrome, optic neuritis, orthostatic hypotension, palinopsia, paresthesia, Parkinson's disease, paramyotonia Congenita, paraneoplastic diseases, paroxysmal attacks, Parry-Romberg syndrome, Pelizaeus-Merzbacher disease, periodic paralyses, peripheral neuropathy, photic sneeze reflex, phytanic acid storage disease, Pick's disease, polymicrogyria (PMG), polymyositis, porencephaly, post-polio syndrome, postherpetic neuralgia (PHN), postural hypotension, Prader-Willi syndrome, primary lateral sclerosis, prion diseases, progressive hemifacial atrophy, progressive multifocal leukoencephalopathy, progressive supranuclear palsy, pseudotumor cerebri, Ramsay Hunt syndrome type I, Ramsay Hunt syndrome type II, Ramsay Hunt syndrome type III, Rasmussen's encephalitis, reflex neurovascular dystrophy, Refsum disease, restless legs syndrome, retrovirus-associated myelopathy, Rett syndrome, Reye's syndrome, rhythmic movement disorder, Romberg syndrome, Saint Vitus dance, Sandhoff disease, schizophrenia, Schilder's disease, schizencephaly, sensory integration dysfunction, septo-optic dysplasia, Shy-Drager syndrome, Sjögren's syndrome, snatiation, Sotos syndrome, spasticity, spina bifida, spinal cord tumors, spinal muscular atrophy, spinocerebellar ataxia, Steele-Richardson-Olszewski syndrome, Stiff-person syndrome, stroke, Sturge-Weber syndrome, subacute sclerosing panencephalitis, subcortical arteriosclerotic encephalopathy, superficial siderosis, Sydenham's chorea, syncope, synesthesia, syringomyelia, tarsal tunnel syndrome, tardive dyskinesia, tardive dysphrenia, Tarlov cyst, Tay-Sachs disease, temporal arteritis, tetanus, tethered spinal cord syndrome, Thomsen disease, thoracic outlet syndrome, tic douloureux, Todd's paralysis, Tourette syndrome, toxic encephalopathy, transient ischemic attack, transmissible spongiform encephalopathies, transverse myelitis, tremor, trigeminal neuralgia, tropical spastic paraparesis, trypanosomiasis, tuberous sclerosis, ubisiosis, Von Hippel-Lindau disease (VHL), Viliuisk Encephalomyelitis (VE), Wallenberg's syndrome, Werdnig, Hoffman disease, west syndrome, Williams syndrome, Wilson's disease and Zellweger syndrome.

The compounds and compositions may also be useful in the inhibition of the development of invasive cancer, tumor angiogenesis and metastasis.

In some embodiments, the disclosure provides a method for treating a disease or disorder associated with aberrant cellular proliferation by administering to a patient in need of such treatment an effective amount of one or more of the compounds of Formula (I), in combination (simultaneously or sequentially) with at least one other agent.

In some embodiments, the disclosure provides a method of treating or ameliorating in a patient a disorder or disease selected from the group consisting of: cancer, pulmonary fibrosis, idiopathic pulmonary fibrosis (IPF), degenerative disc disease, bone/osteoporotic fractures, bone or cartilage disease, and osteoarthritis, the method comprising administering to the patient a therapeutically effective amount of a compound according to claim 1, or a pharmaceutically acceptable salt thereof.

In some embodiments, the pharmaceutical composition comprises a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

In some embodiments, the method of treats a disorder or disease in which aberrant Wnt signaling is implicated in a patient, the method comprises administering to the patient a therapeutically effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the disorder or disease is cancer.

In some embodiments, the disorder or disease is systemic inflammation.

In some embodiments, the disorder or disease is metastatic melanoma.

In some embodiments, the disorder or disease is fatty liver disease.

In some embodiments, the disorder or disease is liver fibrosis.

In some embodiments, the disorder or disease is tendonitis.

In some embodiments, the disorder or disease is damage to a tendon requiring tendon regeneration.

In some embodiments, the disorder or disease is diabetes.

In some embodiments, the disorder or disease is degenerative disc disease.

In some embodiments, the disorder or disease is osteoarthritis.

In some embodiments, the disorder or disease is diabetic retinopathy.

In some embodiments, the disorder or disease is pulmonary fibrosis.

In some embodiments, the disorder or disease is idiopathic pulmonary fibrosis (IPF).

In some embodiments, the disorder or disease is degenerative disc disease.

In some embodiments, the disorder or disease is rheumatoid arthritis.

In some embodiments, the disorder or disease is scleroderma.

In some embodiments, the disorder or disease is a mycotic or viral infection.

In some embodiments, the disorder or disease is a bone or cartilage disease.

In some embodiments, the disorder or disease is Alzheimer's disease.

In some embodiments, the disorder or disease is osteoarthritis.

In some embodiments, the disorder or disease is lung disease

In some embodiments, the disorder or disease is a genetic disease caused by mutations in Wnt signaling components, wherein the genetic disease is selected from: polyposis coli, osteoporosis-pseudoglioma syndrome, familial exudative vitreoretinopathy, retinal angiogenesis, early coronary disease, tetra-amelia syndrome, Müllerian-duct regression and virilization, SERKAL syndrome, diabetes mellitus type 2, Fuhrmann syndrome, Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome, odonto-onycho-dermal dysplasia, obesity, split-hand/foot malformation, caudal duplication syndrome, tooth agenesis, Wilms tumor, skeletal dysplasia, focal dermal hypoplasia, autosomal recessive anonychia, neural tube defects, alpha-thalassemia (ATRX) syndrome, fragile X syndrome, ICF syndrome, Angelman syndrome, Prader-Willi syndrome, Beckwith-Wiedemann Syndrome, Norrie disease and Rett syndrome.

In some embodiments, the patient is a human.

In some embodiments, the cancer is chosen from: hepatocellular carcinoma, colon cancer, breast cancer, pancreatic cancer, chronic myeloid leukemia (CML), chronic myelomonocytic leukemia, chronic lymphocytic leukemia (CLL), acute myeloid leukemia, acute lymphocytic leukemia, Hodgkin lymphoma, lymphoma, sarcoma and ovarian cancer.

In some embodiments, the cancer is chosen from: lung cancer—non-small cell, lung cancer—small cell, multiple myeloma, nasopharyngeal cancer, neuroblastoma, osteosarcoma, penile cancer, pituitary tumors, prostate cancer, retinoblastoma, rhabdomyosarcoma, salivary gland cancer, skin cancer—basal and squamous cell, skin cancer—melanoma, small intestine cancer, stomach (gastric) cancers, testicular cancer, thymus cancer, thyroid cancer, uterine sarcoma, vaginal cancer, vulvar cancer, laryngeal or hypopharyngeal cancer, kidney cancer, Kaposi sarcoma, gestational trophoblastic disease, gastrointestinal stromal tumor, gastrointestinal carcinoid tumor, gallbladder cancer, eye cancer (melanoma and lymphoma), Ewing tumor, esophagus cancer, endometrial cancer, colorectal cancer, cervical cancer, brain or spinal cord tumor, bone metastasis, bone cancer, bladder cancer, bile duct cancer, anal cancer and adrenal cortical cancer.

In some embodiments, the cancer is hepatocellular carcinoma.

In some embodiments, the cancer is colon cancer.

In some embodiments, the cancer is colorectal cancer.

In some embodiments, the cancer is breast cancer.

In some embodiments, the cancer is pancreatic cancer.

In some embodiments, the cancer is chronic myeloid leukemia (CML).

In some embodiments, the cancer is chronic myelomonocytic leukemia.

In some embodiments, the cancer is chronic lymphocytic leukemia (CLL).

In some embodiments, the cancer is acute myeloid leukemia.

In some embodiments, the cancer is acute lymphocytic leukemia.

In some embodiments, the cancer is Hodgkin lymphoma.

In some embodiments, the cancer is lymphoma.

In some embodiments, the cancer is sarcoma.

In some embodiments, the cancer is ovarian cancer.

In some embodiments, the cancer is lung cancer—non-small cell.

In some embodiments, the cancer is lung cancer—small cell.

In some embodiments, the cancer is multiple myeloma.

In some embodiments, the cancer is nasopharyngeal cancer.

In some embodiments, the cancer is neuroblastoma.

In some embodiments, the cancer is osteosarcoma.

In some embodiments, the cancer is penile cancer.

In some embodiments, the cancer is pituitary tumors.

In some embodiments, the cancer is prostate cancer.

In some embodiments, the cancer is retinoblastoma.

In some embodiments, the cancer is rhabdomyosarcoma.

In some embodiments, the cancer is salivary gland cancer.

In some embodiments, the cancer is skin cancer—basal and squamous cell.

In some embodiments, the cancer is skin cancer—melanoma.

In some embodiments, the cancer is small intestine cancer.

In some embodiments, the cancer is stomach (gastric) cancers.

In some embodiments, the cancer is testicular cancer.

In some embodiments, the cancer is thymus cancer.

In some embodiments, the cancer is thyroid cancer.

In some embodiments, the cancer is uterine sarcoma.

In some embodiments, the cancer is vaginal cancer.

In some embodiments, the cancer is vulvar cancer.

In some embodiments, the cancer is Wilms tumor.

In some embodiments, the cancer is laryngeal or hypopharyngeal cancer.

In some embodiments, the cancer is kidney cancer.

In some embodiments, the cancer is Kaposi sarcoma.

In some embodiments, the cancer is gestational trophoblastic disease.

In some embodiments, the cancer is gastrointestinal stromal tumor.

In some embodiments, the cancer is gastrointestinal carcinoid tumor.

In some embodiments, the cancer is gallbladder cancer.

In some embodiments, the cancer is eye cancer (melanoma and lymphoma).

In some embodiments, the cancer is Ewing tumor.

In some embodiments, the cancer is esophagus cancer.

In some embodiments, the cancer is endometrial cancer.

In some embodiments, the cancer is colorectal cancer.

In some embodiments, the cancer is cervical cancer.

In some embodiments, the cancer is brain or spinal cord tumor.

In some embodiments, the cancer is bone metastasis.

In some embodiments, the cancer is bone cancer.

In some embodiments, the cancer is bladder cancer.

In some embodiments, the cancer is bile duct cancer.

In some embodiments, the cancer is anal cancer.

In some embodiments, the cancer is adrenal cortical cancer.

In some embodiments, the disorder or disease is a neurological condition, disorder or disease, wherein the neurological condition/disorder/disease is selected from: Alzheimer's disease, frontotemporal dementias, dementia with lewy bodies, prion diseases, Parkinson's disease, Huntington's disease, progressive supranuclear palsy, corticobasal degeneration, multiple system atrophy, amyotrophic lateral sclerosis (ALS), inclusion body myositis, autism, degenerative myopathies, diabetic neuropathy, other metabolic neuropathies, endocrine neuropathies, orthostatic hypotension, multiple sclerosis and Charcot-Marie-Tooth disease.

In some embodiments, the compound of Formula (I) inhibits one or more proteins in the Wnt pathway.

In some embodiments, the compound of Formula (I) inhibits signaling induced by one or more Wnt proteins.

In some embodiments, the Wnt proteins are chosen from: WNT1, WNT2, WNT2B, WNT3, WNT3A, WNT4. WNT5A, WNT5B, WNT6, WNT7A, WNT7B, WNT8A, WNT8B, WNT9A, WNT9B, WNT10A, WNT10B, WNT11, and WNT16.

In some embodiments, the method inhibits one or more proteins in the Wnt pathway, the method comprises contacting a cell with an effective amount of a compound of Formula (I).

In some embodiments, the cell is a human cell.

In some embodiments, the human cell is a cancerous cell.

In some embodiments, the cancerous cell is a colon cancer cell.

In some embodiments, the contacting is in vitro.

In some embodiments, the compound of Formula (I) inhibits a kinase activity.

In some embodiments, the method treats a disease or disorder mediated by the Wnt pathway in a patient, the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound of Formula (I) inhibits one or more Wnt proteins.

In some embodiments, the method treats a disease or disorder mediated by kinase activity in a patient, the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the disease or disorder comprises tumor growth, cell proliferation, or angiogenesis.

In some embodiments, the method inhibits the activity of a protein kinase receptor, the method comprises contacting the receptor with an effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method treats a disease or disorder associated with aberrant cellular proliferation in a patient; the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method prevents or reduces angiogenesis in a patient; the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method prevents or reduces abnormal cellular proliferation in a patient; the method comprises administering to the patient a therapeutically effective amount of a compound (or compounds) of Formula (I), or a pharmaceutically acceptable salt thereof.

In some embodiments, the method treats a disease or disorder associated with aberrant cellular proliferation in a patient, the method comprises administering to the patient a pharmaceutical composition comprising one or more of the compounds of claim 1 in combination with a pharmaceutically acceptable carrier and one or more other agents.

Moreover, the compounds and compositions, for example, as inhibitors of the cyclin-dependent kinases (CDKs), can modulate the level of cellular RNA and DNA synthesis and therefore are expected to be useful in the treatment of viral infections such as HIV, human papilloma virus, herpes virus, Epstein-Barr virus, adenovirus, Sindbis virus, pox virus and the like.

Compounds and compositions described herein can inhibit the kinase activity of, for example, CDK/cyclin complexes, such as those active in the G_(0.) or G_(0.1) stage of the cell cycle, e.g., CDK2, CDK4, and/or CDK6 complexes.

Evaluation of Biological Activity

The biological activity of the compounds described herein can be tested using any suitable assay known to those of skill in the art, see, e.g., WO 2001/053268 and WO 2005/009997. For example, the activity of a compound may be tested using one or more of the test methods outlined below.

In one example, tumor cells may be screened for Wnt independent growth. In such a method, tumor cells of interest are contacted with a compound (i.e. inhibitor) of interest, and the proliferation of the cells, e.g. by uptake of tritiated thymidine, is monitored. In some embodiments, tumor cells may be isolated from a candidate patient who has been screened for the presence of a cancer that is associated with a mutation in the Wnt signaling pathway. Candidate cancers include, without limitation, those listed above.

In another example, one may utilize in vitro assays for Wnt biological activity, e.g. stabilization of β-catenin and promoting growth of stem cells. Assays for biological activity of Wnt include stabilization of β-catenin, which can be measured, for example, by serial dilutions of a candidate inhibitor composition. An exemplary assay for Wnt biological activity contacts a candidate inhibitor with cells containing constitutively active Wnt/β-catenin signaling. The cells are cultured for a period of time sufficient to stabilize β-catenin, usually at least about 1 hour, and lysed. The cell lysate is resolved by SDS PAGE, then transferred to nitrocellulose and probed with antibodies specific for β-catenin.

In a further example, the activity of a candidate compound can be measured in a Xenopus secondary axis bioassay (Leyns, L. et al. Cell (1997), 88(6), 747-756).

To further illustrate this invention, the following examples are included. The examples should not, of course, be construed as specifically limiting the invention. Variations of these examples within the scope of the claims are within the purview of one skilled in the art and are considered to fall within the scope of the invention as described, and claimed herein. The reader will recognize that the skilled artisan, armed with the present disclosure, and skill in the art is able to prepare and use the invention without exhaustive examples.

EXAMPLES Compound Preparation

The starting materials used in preparing the compounds of the invention are known, made by known methods, or are commercially available. It will be apparent to the skilled artisan that methods for preparing precursors and functionality related to the compounds claimed herein are generally described in the literature. The skilled artisan given the literature and this disclosure is well equipped to prepare any of the compounds.

It is recognized that the skilled artisan in the art of organic chemistry can readily carry out manipulations without further direction, that is, it is well within the scope and practice of the skilled artisan to carry out these manipulations. These include reduction of carbonyl compounds to their corresponding alcohols, oxidations, acylations, aromatic substitutions, both electrophilic and nucleophilic, etherifications, esterification and saponification and the like. These manipulations are discussed in standard texts such as March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure 7^(th) Ed., John Wiley & Sons (2013), Carey and Sundberg, Advanced Organic Chemistry 5^(th) Ed., Springer (2007), Comprehensive Organic Transformations: A Guide to Functional Group Transformations, 2^(nd) Ed., John Wiley & Sons (1999) (incorporated herein by reference in its entirety) and the like.

The skilled artisan will readily appreciate that certain reactions are best carried out when other functionality is masked or protected in the molecule, thus avoiding any undesirable side reactions and/or increasing the yield of the reaction. Often the skilled artisan utilizes protecting groups to accomplish such increased yields or to avoid the undesired reactions. These reactions are found in the literature and are also well within the scope of the skilled artisan. Examples of many of these manipulations can be found for example in T. Greene and P. Wuts Protective Groups in Organic Synthesis, 4th Ed., John Wiley & Sons (2007), incorporated herein by reference in its entirety.

Trademarks used herein are examples only and reflect illustrative materials used at the time of the invention. The skilled artisan will recognize that variations in lot, manufacturing processes, and the like, are expected. Hence the examples, and the trademarks used in them are non-limiting, and they are not intended to be limiting, but are merely an illustration of how a skilled artisan may choose to perform one or more of the embodiments of the invention.

(¹H) nuclear magnetic resonance spectra (NMR) were measured in the indicated solvents on a Bruker NMR spectrometer (Avance™ DRX300, 300 MHz for ¹H or Avance™ DRX500, 500 MHz for ¹H) or Varian NMR spectrometer (Mercury 400BB, 400 MHz for ¹H). Peak positions are expressed in parts per million (ppm) downfield from tetramethylsilane. The peak multiplicities are denoted as follows, s, singlet; d, doublet; t, triplet; q, quartet; ABq, AB quartet; quin, quintet; sex, sextet; sep, septet; non, nonet; dd, doublet of doublets; ddd, doublet of doublets of doublets; d/ABq, doublet of AB quartet; dt, doublet of triplets; td, triplet of doublets; dq, doublet of quartets; m, multiplet.

The following abbreviations have the indicated meanings:

BH₃-Me₂S=borane dimethyl sulfide complex

(Boc)₂O=di-tert-butyl dicarbonate

brine=saturated aqueous sodium chloride

CDCl₃=deuterated chloroform

CD₃OD=deuterated methanol

DCAD=di-(4-chlorobenzyl)azodicarboxylate

DCE=dichloroethane

DCM=dichloromethane

DEAD=diethyl azodicarboxylate

DHP=dihydropyran

DMAP=4-dimethylaminopyridine

DMF=N,N-dimethylformamide

DMSO-d₆=deuterated dimethylsulfoxide

ESIMS=electron spray mass spectrometry

EtOAc=ethyl acetate

EtOH=ethanol

HCl=hydrochloric acid

HOAc=acetic acid

K₂CO₃=potassium carbonate

KOAc=potassium acetate

LDA=lithium diisopropylamide

LC/MS=liquid chromatography-mass spectrometry

MeOH=methanol

MgSO₄=magnesium sulfate

MsC1=methanesulfonyl chloride or mesyl chloride

MW=microwave

NaBH₄=sodium borohydride

NaBH(OAc)₃=sodium triacetoxyborohydride

NaCNBH₃=sodium cyanoborohydride

NaHCO₃=sodium bicarbonate

NaOH=sodium hydroxide

Na₂S₂O₅=sodium metabisulfite or sodium pyrosulfite

NH₄OH=ammonium hydroxide

NMR=nuclear magnetic resonance

ON=overnight

Pd/C=palladium(0) on carbon

Pd(dppf)Cl₂=1,1′-bis(diphenylphosphino)ferrocene]palladium(II) chloride

Pd(PPh₃)₄=tetrakis(triphenylphosphine)palladium(0)

Pd(PPh₃)₂Cl₂=bis(triphenylphosphine)palladium(II) dichloride

PE=petroleum ether

Pin₂B₂=bis(pinacolato)diboron

PPh₃=triphenylphosphine

PPTS=pyridinium p-toluenesulfonate

r.t.=room temperature

SEM-Cl=2-(trimethylsilyl)ethoxymethyl chloride

TEA=triethylamine

TFA=trifluoroacetic acid

THF=tetrahydrofuran

THP=tetrahydropyran

TLC=thin layer chromatography

p-TsOH=p-toluenesulfonic acid

The following example schemes are provided for the guidance of the reader, and collectively represent an example method for making the compounds provided herein. Furthermore, other methods for preparing compounds of the invention will be readily apparent to the person of ordinary skill in the art in light of the following reaction schemes and examples. The skilled artisan is thoroughly equipped to prepare these compounds by those methods given the literature and this disclosure. The compound numberings used in the synthetic schemes depicted below are meant for those specific schemes only, and should not be construed as or confused with same numberings in other sections of the application. Unless otherwise indicated, all variables are as defined above.

General Procedure

Compounds of Formula (I) of the present invention can be prepared as depicted in Scheme 1.

Scheme 1 describes a method for preparation of 1H-pyrazolo[3,4-c]pyridine derivatives (XI) by first acylating a 2-bromo-5-fluoropyridine (II) with an ethyl 2,2-dialkoxyacetate to produce the acetal protected oxoacetaldehyde (III). The keto group was then converted to the Boc-protected hydrazone (IV) followed by base cyclization to the 1H-pyrazolo[3,4-c]pyridine (V). The pyrazolopyridine (V) is then protected with either a Boc or THP (VI) followed by Suzuki coupling with various boronic acids (VII). The pyrazolopyridine acetyl (VIII) is reacted with various 1,2-diamines (IX) to produce (X). Final deprotection of the pyrazole nitrogen yields the desired 1H-pyrazolo[3,4-c]pyridine derivatives (XI).

Illustrative Compound Examples

Preparation of Boc-protected intermediate (XVI) is depicted below in Scheme 2.

Step 1

To a solution of 2-bromo-5-fluoro-pyridine (XII) (100.0 g, 568.21 mmol, 1.0 eq) in THF (1000 mL) was added a solution of LDA (66.95 g, 625.04 mmol, 1.10 eq) drop-wise at −78° C. over a period of 1 h under N₂. The reaction mixture was stirred at −78° C. for 30 min. Then a solution of ethyl 2,2-diethoxyacetate (120.15 g, 681.86 mmol, 1.20 eq) was added at −78° C. over a period of 1 h. The reaction mixture was stirred at −78° C. for another 1 h. TLC (PE:EtOAc=10:1) showed that the starting material was consumed completely. The reaction was quenched by NH₄Cl slowly and then extracted with EtOAc (1000 mL×2). The combined organic phase was washed with saturated brine (500 mL×2), dried over anhydrous Na₂SO₄, filtered and concentrated in vacuo. The residue was purified by chromatography on silica gel (PE:EtOAc=10:1) to give 1-(2-bromo-5-fluoropyridin-4-yl)-2,2-diethoxyethan-1-one (XIII) (160.0 g, 522.65 mmol, 92.0% yield) as a yellow solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.26 (t, J=6.8 Hz, 6H), 3.69 (q, J=7.2 Hz, 2H), 3.79 (q, J=7.2 Hz, 2H), 5.23 (d, J=2 Hz, 1H), 7.85 (d, J=4.8 Hz, 1H), 8.39 (d, J=1.2 Hz, 1H); ESIMS found for C₁₁H₁₃BrFNO₃ m/z 306.1 (M+H).

Step 2

To a mixture of 1-(2-bromo-5-fluoropyridin-4-yl)-2,2-diethoxyethan-1-one (XIII) (142.0 g, 463.86 mmol, 1.0 eq) in THF (2 L) tert-butyl hydrazinecarboxylate (61.30 g, 463.86 mmol, 1.0 eq) in one portion at room temperature. The mixture was stirred at 55° C. for 60 h. TLC (PE:EtOAc=2:1) showed that most of the starting material was consumed. The crude mixture of tert-butyl 2-(1-(2-bromo-5-fluoropyridin-4-yl)-2,2-diethoxyethylidene)hydrazine-1-carboxylate (XIV) was used in the next step without further purification.

Step 3

To a solution of tert-butyl 2-(1-(2-bromo-5-fluoropyridin-4-yl)-2,2-diethoxyethylidene)hydrazine-1-carboxylate (XIV) (190.0 g, 452.09 mmol, 1.0 eq) in THF (2 L) was added NaH (36.17 g, 904.18 mmol, 2.0 eq) in portions at 0° C. over 0.5 h. The mixture was stirred at 55° C. for 4 hours. TLC (PE:EtOAc=2:1) showed the material was consumed completely. The mixture was cooled to 0° C. The mixture was poured into 10% aqueous NH₄Cl (1000 mL). The aqueous phase was extracted with EtOAc (800 mL×3). The combined organic phase was washed with saturated brine (800 mL), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=10:1→5:1→1:4) to afford 5-bromo-3-(diethoxymethyl)-1H-pyrazolo[3,4-c]pyridine (XV) (67.0 g, 223.22 mmol, 49.4% yield) as yellow solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.30 (t, J=7.03 Hz, 6H), 3.60-3.86 (m, 4H), 5.98 (s, 1H), 8.12 (d, J=1.13 Hz, 1H), 8.96 (s, 1H), 11.82 (brs, 1H); ESIMS found for C₁₁H₁₄BrN₃O₂ m/z 300.0 (M+H).

Step 4

To a solution of 5-bromo-3-(diethoxymethyl)-1H-pyrazolo[3,4-c]pyridine (XV) (20.0 g, 66.63 mmol, 1.0 eq) in CH₃CN (100 mL) was added Boc₂O (21.81 g, 99.95 mmol, 1.5 eq), DMAP (814.06 mg, 6.66 mmol, 0.10 eq) and TEA (13.49 g, 133.27 mmol, 2.0 eq) at room temperature. The mixture was stirred at 15° C. for 1 hr. TLC (PE:EtOAc=5:1) showed that starting the material was consumed completely. The mixture was added water (50 mL) and extracted with EtOAc (40 mL×2). The organic layers were washed with brine (60 mL) and concentrated under vacuum. The residue was purified by chromatography on silica gel (PE:EtOAc=10:1) to produce tert-butyl 5-bromo-3-(diethoxymethyl)-1H-pyrazolo[3,4-c]pyridine-1-carboxylate (XVI) (23.70 g, 59.21 mmol, 88.9% yield) as a light yellow oil. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.29 (t, J=6.8 Hz, 6H), 1.75 (s, 9H), 3.60-3.71 (m, 2H), 3.75-3.87 (m, 2H), 5.79 (s, 1H), 8.14 (d, J=1.00 Hz, 1H), 9.25 (s, 1H); ESIMS found for C₁₆H₂₂BrN₃O₄ m/z 400.0 (M+H).

Preparation of THP protected intermediate (XVIII) is depicted below in Scheme 3.

Step 1

To a mixture of 5-bromo-3-(dimethoxymethyl)-1H-pyrazolo[3,4-c]pyridine (XVII) (26.0 g, 69.85 mmol, 1.0 eq) and 3,4-dihydro-2H-pyran (14.69 g, 174.63 mmol, 2.5 eq) in toluene (100 mL) was added 4-methylbenzenesulfonic acid (2.41 g, 13.97 mmol, 0.20 eq) in one portion at room temperature under N₂. The mixture was heated to 90° C. and stirred for 2 hr. LC/MS showed the reaction was completed. the mixture was extracted with EtOAc (100 mL×3), washed with water (50 mL×2) and brine (50 mL×2). The organic layer was dried and concentrated to give a residue. the residue was purified by a column (PE:EtOAc=10:1-8:1-5:1) to give 5-bromo-1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-c]pyridine-3-carbaldehyde (XVIII) (8.50 g, 30.65 mmol, 43.9% yield). ¹H NMR (CDCl₃, 500 MHz) δ ppm 1.68-1.80 (m, 2H), 1.80-1.91 (m, 1H), 2.08-2.27 (m, 2H), 2.41-2.56 (m, 1H), 3.77-3.88 (m, 1H), 3.90-4.00 (m, 1H), 5.93 (dd, J=2.8 Hz, J=8 Hz, 1H), 8.31 (s, 1H), 9.01 (s, 1H), 10.24 (s, 1H); ESIMS found C₁₂H₁₂BrN₃O₂ m/z 310.1 (M+H).

Preparation of intermediate N-(5-bromopyridin-3-yl)pivalamide (XXI) is depicted below in Scheme 4.

Step 1

To a solution of 3-amino-5-bromo pyridine (XIX) (1.0 g, 5.78 mmol) in dry pyridine (10 mL) was added pivaloyl chloride (XX) (769 mg, 6.38 mmol). The reaction mixture was stirred at room temperature for 3 h. The reaction was poured into an ice water/saturated aqueous NaHCO₃ mixture and stirred for 30 min. The precipitate was filtered, washed with cold water and dried at room temperature to yield N-(5-bromopyridin-3-yl)pivalamide (XXI) as an off-white solid (1.082 g, 4.22 mmol, 73.1% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.23 (s, 9H), 8.37 (d, J=2 Hz, 1H), 8.39 (t, J=2 Hz, 1H), 8.80 (d, J=2 Hz, 1H), 9.58 (brs, 1H); ESIMS found C₁₀H₁₃BrN₂O m/z 258.9 (Br⁸¹M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 4.

N-(5-Bromopyridin-3-yl)isobutyramide (XXII): Off-white solid, (71% yield). ¹H NMR (CDCl₃) δ ppm 8.55-8.35 (m, 3H), 7.32 (s, 1H), 2.59-2.48 (m, 1H), 1.28-1.27 (d, 6H); ESIMS found C₉H₁₁BrN₂O m/z 242.9 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)propionamide (XXIII): Off white solid (92% yield). ¹H NMR (DMSO-d₆) δ ppm 1.09 (t, J=7.54 Hz, 3H), 2.36 (q, J=7.54 Hz, 2H), 8.36 (m, 2H), 8.65 (d, J=2.07 Hz, 1H), 10.26 (s, 1H); ESIMS found C₈H₉BrN₂O m/z 231.1 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)butyramide (XXIV): Yellow solid (2.1 g, 8.64 mmol, 88.8% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.02 (t, J=7.2 Hz, 3H), 1.74 (sxt, J=7.2 Hz, 2H), 2.40 (t, J=7.2 Hz, 2H), 8.35 (d, J=2 Hz, 1H), 8.46 (t, J=2 Hz, 1H), 8.63 (d, J=2 Hz, 1H); ESIMS found C₉H₁₁BrN₂O m/z 243.1 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)pentanamide (XXV): Yellow solid (2.0 g, 7.78 mmol, 85.3% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 0.98 (t, J=7.4 Hz, 3H), 1.43 (sxt, J=7.4 Hz, 2H), 1.70 (quin, J=7.4 Hz, 2H), 2.43 (t, J=7.6 Hz, 2H), 8.35 (s, 1H), 8.45 (d, J=2 Hz, 1H), 8.64 (d, J=2 Hz, 1H); ESIMS found C₁₀H₁₃BrN₂O m/z 256.9 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)-3-methylbutanamide (XXVI): Off white solid, (67% yield), ¹H NMR (CDCl₃, 500 MHz) δ ppm 8.55-8.42 (m, 3H), 7.62 (s, 1H), 2.31-2.18 (m, 3H), 1.02-1.01 (d, J=6 Hz, 6H); ESIMS found C₁₀H₁₃BrN₂O m/z 258.9 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)-3,3-dimethylbutanamide (XXVII): Yellow solid (1.7 g, 6.27 mmol, 78.6% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.10 (s, 9H), 2.29 (s, 2H), 8.36 (d, J=1.6 Hz, 1H), 8.46 (d, J=2.0 Hz, 1H), 8.64 (d, J=2.0 Hz, 1H); ESIMS found C₁₁H₁₅BrN₂O m/z 273.1 ((Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)-2-phenylacetamide (XXVIII): White solid (2.5 g, 8.59 mmol, 77.9% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 3.76 (s, 2H), 7.26-7.45 (m, 5H), 7.57 (brs, 1H), 8.33 (s, 1H), 8.37 (s, 2H); ESIMS found C₁₃H₁₁BrN₂O m/z 292.8 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)benzamide (XXIX): White solid (2.7 g, 9.74 mmol, 60% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 7.40-7.52 (m, 2H), 7.52-7.62 (m, 1H), 7.86 (d, J=7.2 Hz, 2H), 8.39 (d, J=1.6 Hz, 1H), 8.46 (s, 1H), 8.55 (d, J=1.6 Hz, 1H), 8.57 (d, J=2.0 Hz, 1H); ESIMS found C₁₂H₉BrN₂O m/z 278.8 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)cyclopropanecarboxamide (XXX): Off-white solid, (83% yield), ¹H NMR (CDCl₃, 500 MHz) δ ppm 8.46-8.39 (m, 3H), 7.54 (bs, 1H), 1.56-1.50 (m, 1H), 1.13-1.07 (m, 2H), 0.96-0.90 (m, 2H); ESIMS found for C₉H₉BrN₂O m/z 240.9 (Br⁷⁹M+H).

N-(5-Bromopyridin-3-yl)cyclobutanecarboxamide (XXXI): Yellow solid (2.1 g, 6.27 mmol, 86.6% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.80-1.99 (m, 1H), 1.99-2.15 (m, 1H), 2.16-2.30 (m, 2H), 2.30-2.45 (m, 2H), 3.25-3.35 (m, 1H), 8.34 (d, J=2.0 Hz, 1H), 8.47 (s, 1H), 8.64 (d, J=2.0 Hz, 1H); ESIMS found C₁₀H₁₁BrN₂O m/z 257.1 (Br⁸¹M+H).

N-(5-Bromopyridin-3-yl)cyclopentanecarboxamide (XXXII): Yellow solid (1.9 g, 7.06 mmol, 80.2% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.57-1.74 (m, 2H), 1.74-1.91 (m, 4H), 1.91-2.07 (m, 2H), 2.77-2.92 (m, 1H), 8.34 (d, J=1.6 Hz, 1H), 8.45 (s, 1H), 8.65 (d, J=2.0 Hz, 1H); ESIMS found C₁₁H₁₃BrN₂O m/z 271.1 (Br⁸¹M+H).

N-(5-bromopyridin-3-yl)cyclohexanecarboxamide (XXXIII): Yellow solid (2.0 g, 7.06 mmol, 84.3% yield). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.19-1.46 (m, 3H), 1.46-1.63 (m, 2H), 1.74 (d, J=11.6 Hz, 1H), 1.88 (t, J=14.0 Hz, 4H), 2.40 (tt, J=11.6 Hz, J=3.6 Hz, 1H), 8.34 (d, J=2.0 Hz, 1H), 8.44 (t, J=2.0 Hz, 1H), 8.64 (d, J=2.0 Hz, 1H); ESIMS found C₁₂H₁₅BrN₂O m/z 285.1 (Br⁸¹M+H).

N-(5-bromopyridin-3-yl)-2-cyclohexylacetamide (XXXIV): Yellow solid (261 mg, 0.878 mmol, 84.4% yield). ESIMS found C₁₃H₁₇BrN₂O m/z 297.1 (Br⁸¹M+H).

Preparation of intermediate 5-bromo-N,N-dimethylpyridin-3-amine (XXXVI) is depicted below in Scheme 5.

Step 1

To a solution of 3,5-dibromopyridine (XXXV) (2.37 g, 10.0 mmol) in dry DMF (20.0 mL) was added K₂CO₃ (4.5 g, 33 mmol) and dimethylamino hydrochloride (1.79 g, 22 mmol). The mixture was heated overnight at 200° C. in a sealed tube. The solution was cooled to room temperature and excess DMF was removed under vacuum. The residue was partitioned between EtOAc and water. The organic phase was separated. The aqueous phase was washed with EtOAc and the combined organic phases were dried over MgSO₄, and concentrated to afford 5-bromo-N,N-dimethylpyridin-3-amine (XXXVI) as an off-white solid (1.78 g, 8.85 mmol, 88% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.94 (s, 6H), 7.25 (t, J=2 Hz, 1H), 7.91 (d, J=2 Hz, 1H), 8.07 (d, J=2 Hz, 1H); ESIMS found C₇H₉BrN₂ m/z 201.1 (M+H).

Preparation of intermediate 5-bromo-N-isopropylpyridin-3-amine (XXXVII) is depicted below in Scheme 6.

Steps 1

To a solution of 5-bromopyridin-3-amine (XIX) (535 mg, 3.09 mmol) in MeOH (62 mL) was added acetone (296 μL, 4.02 mL). The pH was adjusted to 4 using HOAc and stirred for 30 min. NaCNBH₃ (272 mg, 4.33 mmol) was added and stirred at room temperature overnight. The MeOH was removed under vacuum and the residue was partitioned between EtOAc and saturated aqueous NaHCO₃. The organic layer was dried over MgSO₄ and evaporated under vacuum. The crude product was purified on a silica gel column (100% hexane→90:10 hexane:EtOAc) to produce 5-bromo-N-isopropylpyridin-3-amine (XXXVII) as an oil which slowly solidified into an off-white solid (309 mg, 1.44 mmol, 47% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.12 (d, J=6.3 Hz, 6H), 3.55-3.59 (m, 1H), 6.03 (d, J=7.9 Hz, 1H), 7.05-7.06 (m, 1H), 7.75 (d, J=2 Hz, 1H), 7.90 (d, J=2 Hz, 1H); ESIMS found C₈H₁₁BrN₂ m/z 215.1 (M+H).

Preparation of intermediate 1-(5-bromopyridin-3-yl)-N,N-dimethylmethanamine (XXXIX) is depicted below in Scheme 7.

Steps 1

Preparation of 1-(5-bromopyridin-3-yl)-N,N-dimethylmethanamine (XXXIX) was performed following the procedure listed in Scheme 6, Step 1. Brown oil (1.20 g, 5.59 mmol, 45% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.15 (s, 6H), 3.43 (s, 2H), 7.94 (s, 1H), 8.47 (d, J=1.1 Hz, 1H), 8.59 (d, J=2.2 Hz, 1H); ESIMS found C₈H₁₁BrN₂ m/z 215 (M^(Br79)+H) and 217 (M^(Br81)+H).

Preparation of intermediate 3-bromo-5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridine (XL) is depicted below in Scheme 8.

Steps 1

To a mixture of 5-bromopyridine-3-carbaldehyde (XXXVIII) (6.00 g, 32.26 mmol, 1.0 eq), 3,3-difluoropyrrolidine (5.56 g, 38.71 mmol, 1.20 eq) and TEA (5.39 mL, 38.71 mmol, 1.2 Eq) in DCE (200 mL) was stirred at room temperature for 30 min, then added sodium triacetoxyborohydride (10.25 g, 48.38 mmol, 1.50 eq) in one portion at room temperature under N₂. The mixture was stirred at room temperature for 6 hours. TLC showed the reaction was complete. The reaction was quenched with 1N NaOH (100 mL), extracted with DCE (100 mL×2). The combined organic layers were washed with brine (100 mL), dried and concentrated. The residue was purified by silica gel chromatography (column height: 50 mm, diameter: 50 mm, 300-400 mesh silica gel, DCM/MeOH=30/1→20/1) to give 3-bromo-5-((3,3-difluoropyrrolidin-1-yl)methyl) pyridine (XL): Yellow oil (8.00 g, 28.9 mmol, 89.5% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.30 (spt, J=7.2 Hz. 2H), 2.75 (t, J=6.8 Hz, 2H), 2.91 (t, J=13.2 Hz, 2H), 7.85 (s, 1H), 8.45 (s, 1H), 8.59 (d, J=2 Hz, 1H); ESIMS found for C₁₀H₁₁BrF₂N₂ m/z 277.0 (M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 7 or Scheme 8.

3-Bromo-5-(pyrrolidin-1-ylmethyl)pyridine (XLI): Golden liquid (1.35 g, 97% yield). ¹H NMR (DMSO-d₆) 1.68-1.71 (m, 4H), 2.42-2.44 (m, 4H), 3.60 (s, 2H), 7.96 (s, 1H), 8.48 (d, J=2 Hz, 1H), 8.58 (d, J=3 Hz, 1H); ESIMS found for C₁₀H₁₃BrN₂ m/z 242.2 (M+H).

3-Bromo-5-(piperidin-1-ylmethyl)pyridine (XLII): Brown liquid (13.1 g, 94% yield). ¹H NMR (DMSO-d₆) 1.36-1.39 (m, 2H), 1.46-1.51 (m, 4H), 2.31-2.32 (m, 4H), 3.46 (s, 2H), 7.94 (s, 1H), 8.47 (d, J=2 Hz, 1H), 8.58 (d, J=3 Hz, 1H); ESIMS found for C₁₁H₁₅BrN₂ m/z 257.0 (M+H).

N-((5-Bromopyridin-3-yl)methyl)ethanamine (XLIII): Golden liquid (1.29 g, 6.00 mmol, 60% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.14 (t, J=7.2 Hz, 3H), 2.67 (q, J=7.2 Hz, 2H), 3.79 (s, 2H), 7.85 (t, J=2 Hz, 1H), 8.46 (d, J=1.6 Hz, 1H), 8.56 (d, J=2.4 Hz, 1H); ESIMS found for C₈H₁₁BrN₂ m/z 215.1 (M+H).

N-Benzyl-1-(5-bromopyridin-3-yl)methanamine (XLIV): Yellow oil (8.0 g, 28.9 mmol, 89.5% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.71 (s, 2H), 3.74 (s, 2H), 7.18-7.28 (m, 1H), 7.28-7.40 (m, 4H), 8.04 (s, 1H), 8.52 (s, 1H), 8.58 (s, 1H); ESIMS found for C₁₃H₁₃BrN₂ m/z 277.1 (M+H).

Preparation of intermediate tert-butyl (5-bromopyridin-3-yl)methyl (cyclopentylmethyl)carbamate (XLIX) is depicted below in Scheme 9.

Step 1

To a solution of 5-bromonicotinaldehyde (XXXVIII) (2.0 g, 10.8 mmol, 1 eq) in MeOH (20 mL) was added NaBH₄ (2.4 g, 64.9 mmol, 6 eq) and the reaction mixture was stirred at room temperature for 3 h. The mixture was concentrated in vacuo and the residue was diluted in water (15 mL), the aqueous phase was extracted with DCM (10 mL×3). The combined organic layers were dried over MgSO₄, filtered and concentrated in vacuo to afford (5-bromopyridin-3-yl)methanol (XLV) (1.8 g, 9.57 mmol, 90.0% yield) as a colorless oil. ¹H NMR (CDCl₃, 500 MHz) δ ppm 4.73 (s, 2H), 7.90 (s, 1H), 8.47 (s, 1H), 8.57 (s, 1H). ESIMS found for C₆H₆BrNO m/z 188.0 (M+H).

Step 2

To a stirred solution of (5-bromopyridin-3-yl)methanol (XLV) (1.60 g, 8.5 mmol, 1 eq), phthalimide (1.24 g, 8.5 mmol, 1 eq) and PPh₃ (3.33 g, 12.75 mmol, 1.5 eq) in anhydrous THF (15 mL) was added DEAD (2.21 g, 12.75 mmol, 1.5 eq) dropwise at 0° C. under N₂. Then the reaction mixture was stirred at room temperature for 6 h. The mixture was washed with saturated NaHCO₃ solution (15 mL), water (15 mL) and brine (15 mL) subsequently. The organic layers were dried over MgSO₄, concentrated under reduced pressure, the resultant residue was purified by flash chromatography on silica gel (PE:EtOAc=4:1) to give 2-((5-bromopyridin-3-yl)methyl)isoindoline-1,3-dione (XLVI) (2.5 g, 7.88 mmol, 82.3% yield) as a white solid. ESIMS found for C₁₄H₉BrN₂O₂ m/z 317.1 (M+H).

Step 3

A solution of 2-((5-bromopyridin-3-yl)methyl)isoindoline-1,3-dione (XLVI) (1.9 g, 6.0 mmol, 1 eq) and hydrazine hydrate (2.0 g, 40 mmol, 6 eq) in EtOH (20 mL) was heated at 70° C. for 3 h. The mixture was filtered through a Celite® pad and the filtrate was concentrated in vacuo, the crude product was dissolved in 1N HCl solution (15 mL) and concentrated to dryness, then it was washed with acetone (10 mL×3), the precipitate was collected by filtration, dried in vacuo to give (5-bromopyridin-3-yl)methanamine (XLVII) (1.3 g, 6.95 mmol, 97.7% yield) as a white solid. ¹H NMR (D₂O, 500 MHz) δ ppm 4.34 (s, 2H), 8.56 (s, 1H), 8.75 (d, J=1.2 Hz, 1H), 8.91 (d, J=1.6 Hz, 1H). ESIMS found for C₆H₇BrN₂ m/z 187.0 (M+H).

Step 4

A solution of (5-bromopyridin-3-yl)methanamine (XLVII) (1.30 g, 5.8 mmol, 1.0 eq), cyclopentanecarbaldehyde (0.57 g, 5.8 mmol, 1.0 eq) and TEA (0.60 g, 5.8 mmol, 1.0 eq) in MeOH (15 mL) was stirred at room temperature for 2 h. Then NaBH₃CN (1.98 g, 34.6 mmol, 6.0 eq) was added and the mixture was stirred at the same temperature for another 3 h. The solvent was removed under reduced pressure and the residue was diluted in water (20 mL) and extracted with DCM (10 mL×3), combined organic layers were dried over MgSO₄ and concentrated in vacuo to give 1-(5-bromopyridin-3-yl)-N-(cyclopentylmethyl)methanamine (XLVIII) (1.23 g, 4.57 mmol, 79.3% yield) as a yellow oil. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.07-1.23 (m, 2H), 1.47-1.67 (m, 4H), 1.70-1.84 (m, 2H), 2.02 (spt, J=7.6 Hz. 1H), 2.53 (d, J=7.2 Hz, 2H), 3.80 (s, 2H), 7.86 (s, 1H), 8.47 (s, 1H), 8.56 (d, J=2.0 Hz, 1H); ESIMS found for C₁₂H₁₇BrN₂ m/z 269.1 (M+H).

Step 5

To a solution of 1-(5-bromopyridin-3-yl)-N-(cyclopentylmethyl) methanamine (XLVIII) (1.00 g, 3.7 mmol, 1 eq) and TEA (0.93 g, 9.2 mmol, 2.5 eq) in DCM (20 mL) was added portionwise (Boc)₂O (0.85 g, 4.0 mmol, 1.1 eq) at 0° C., the reaction mixture was stirred at room temperature for 1 h. The mixture was washed with water (10 mL), brine (10 mL), the organic layer was separated, dried over MgSO₄ and concentrated in vacuo to give tert-butyl (5-bromopyridin-3-yl)methyl (cyclopentylmethyl)carbamate (XLIX) (1.25 g, 3.38 mmol, 91.9% yield) as a white solid. ESIMS found for C₁₇H₂₅BrN₂O₂ m/z 369.1 (M+H).

Preparation of intermediate 3-bromo-5-(cyclohexyloxy)pyridine (LII) is depicted below in Scheme 10.

Step 1

To a solution of 5-bromopyridin-3-ol (L) (523 mg, 3.01 mmol) in THF (30 mL) cooled to 0° C. were added triphenylphosphine (867 mg, 3.31 mmol) and cyclohexanol (LI) (331 mg, 3.31 mmol) followed by (E)-bis(4-chlorobenzyl) diazene-1,2-dicarboxylate (1.21 g, 3.31 mmol), added portionwise. The reaction mixture was then stirred at 25° C. overnight. The reaction was worked-up with a EtOAc—NaHCO₃ extraction and the solid filtered off. The solvent was removed and the residue was purified by Isco (20% EtOAc-Hexanes) to give 3-bromo-5-(cyclohexyloxy)pyridine (LII) (209 mg, 0.82 mmol, 27.2% yield) as a yellow oil. ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.21-1.31 (m, 1H) 1.34-1.48 (m, 4H) 1.49-1.57 (m, 1H) 1.70 (br dd, J=9.74, 4.25 Hz, 2H) 1.88-1.96 (m, 2H) 2.50 (dt, J=3.70, 1.72 Hz, 5H) 4.46-4.54 (m, 1H) 7.72 (t, J=2.20 Hz, 1H) 8.24 (d, J=1.92 Hz, 1H) 8.27 (d, J=2.47 Hz, 1H).

The following intermediate was prepared in accordance with the procedure described in the above Scheme 10.

tert-Butyl 4-((5-bromopyridin-3-yl)oxy)piperidine-1-carboxylate (LIII): Yellow oil (244 mg, 0.683 mmol, 23.2% yield). ESIMS found for C₁₅H₂₁BrN₂O₃ m/z 358.3 (M+H).

Preparation of intermediate 3-(benzyloxy)-5-bromopyridine (LV) is depicted below in Scheme 11.

Step 1

To a solution of 5-bromopyridin-3-ol (L) (174 mg, 1.0 mmol) in DMF (3 mL) was added potassium carbonate (415 mg, 3.0 mmol). The slurry was heated at 90° C. for 1 hour and then cooled to 25° C. The (bromomethyl)benzene (LIV) (171 mg, 1.0 mmol) was added and the mixture was stirred at 25° C. overnight. The reaction was worked-up using a saturated sodium bicarbonate and ethyl acetate extraction. The product was purified by ISCO column eluted with 40-100% EtOAc-Hexanes. The 3-(benzyloxy)-5-bromopyridine (LV) (105 mg, 0.398 mmol, 39.8% yield) was obtained as yellow oil. MS: 266.1. ESIMS found for C₁₂H₁₀BrNO m/z 266.1 (M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 11.

3-Bomo-5-(2-(pyrrolidin-1-yl)ethoxy)pyridine (LVI): Yellow oil ((97 mg, 0.358 mmol, 15.56% yield). ESIMS found for C₁₁H₁₅BrN₂O m/z 272.2 (M+H).

2-((5-bromopyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (LVII): Yellow oil (97 mg, 0.396 mmol, 28.9% yield). ESIMS found for C₉H₁₃BrN₂O m/z 245.1 (M+H).

1-(2-(3-bromo-5-fluorophenoxy)ethyl)pyrrolidine (LVIII): Yellow oil (370 mg, 1.284 mmol, 85.8% yield). ESIMS found for C₁₂H₁₅BrFNO m/z 289.0 (M+H).

2-(3-bromo-5-fluorophenoxy)-N,N-dimethylethan-1-amine (LIX): Yellow oil (364 mg, 1.389 mmol, 50.2% yield). ESIMS found for C₁₀H₁₃BrFNO m/z 263.9 (M+H).

Preparation of intermediate tert-butyl 4-(2-((5-bromopyridin-3-yl)amino)-2-oxoethyl)piperidine-1-carboxylate (LXI) is depicted below in Scheme 12.

Step 1

To a solution of 2-(1-(tert-butoxycarbonyl)piperidin-4-yl)acetic acid (LX) (3.4 g, 13.97 mmol) in DCM (10 mL) was added DMF (1 mL). The solution was cooled in ice-water to 0° C. Oxalyl chloride (1.835 mL, 20.96 mmol) was then added dropwise. The mixture was stirred for one hour at 25° C. The organic volatile was then removed under vacuum. The residue was dissolved in DCM (10 mL). DMAP (0.171 g, 1.397 mmol) and 5-bromopyridin-3-amine (XIX) (2.418 g, 13.97 mmol) were added to the solution and cooled to 0° C. DIEA (4.88 ml, 27.9 mmol) was then added dropwise and the mixture was stirred for 2 hours at 25° C. The reaction was worked-up with DCM and saturated NaHCO₃. The product was purified by ISCO eluted with 0-100% EtOAc-Hexanes. The tert-butyl 4-(2-((5-bromopyridin-3-yl)amino)-2-oxoethyl)piperidine-1-carboxylate (LXI) (2.82 g, 7.08 mmol, 50.7% yield) was obtained as yellow oil. ESIMS found for C₁₇H₂₄BrN₃O₃ m/z 343.1 (M-56).

The following intermediate was prepared in accordance with the procedure described in the above Scheme 12.

N-(5-Bromopyridin-3-yl)-2-(dimethylamino)acetamide (LXII): Yellow oil (528 mg, 2.05 mmol, 19.0% yield). ESIMS found for C₉H₁₂BrN₃O m/z 259.3 (M+H).

Preparation of tert-butyl (1-(6-chloropyrazin-2-yl)azetidin-3-yl)carbamate (LXV) is depicted below in Scheme 13.

Step 1

To a solution of tert-butyl azetidin-3-ylcarbamate hydrochloride (LXIII) (2 g, 9.58 mmol) in dry DMF (19.2 mL) was added DIPEA (8.37 ml, 47.9 mmol). To this mixture was added 2,6-dichloropyrazine (LXIV) (1.428 g, 9.58 mmol) and the reaction was stirred at 95° C. for 3 hours. The reaction was quenched with water (20 mL) and extracted with EtOAc. The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by silica gel column chromatography (40 g) (100% hexanes-*hexanes:EtOAc 1:1) to yield tert-butyl (1-(6-chloropyrazin-2-yl)azetidin-3-yl)carbamate (LXV) (2.2882 g, 8.04 mmol, 84% yield) as a white solid. ESIMS found for C₁₂H₁₇ClN₄O₂ m/z 285.1 (M+H).

Preparation of intermediate 2′-fluorobiphenyl-2,3-diamine (LXIX) is depicted below in Scheme 14.

Step 1

A solution of 3-bromo-2-nitroaniline (LXVI) (2.00 g, 9.30 mmol, 1 eq), 2-fluorophenylboronic acid (LXVII) (1.42 g, 10.14 mmol, 1.1 eq), Pd(PPh₃)₄ (0.35 g, 0.03 mmol, 0.03 eq), Na₂CO₃ (1.95 g, 18.40 mmol, 2 eq) in a mixed solvent of toluene (15 mL), H₂O (9 mL) and EtOH (3 ml) was stirred at 75° C. for 15 h under nitrogen atmosphere. Then the reaction mixture was washed with brine (20 mL) and dried over anhydrous Na₂SO₄, filtered and concentrated in vacuo, the resultant residue was purified by chromatography on silica gel (PE:EtOAc=3:1) to afford 2′-fluoro-2-nitrobiphenyl-3-amine (LXVIII) (1.0 g, 4.30 mmol, 46.6% yield) as a yellow solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 6.54 (d, J=6.4 Hz, 1H), 6.64 (s, 2H), 7.04 (dd, J=8.8 Hz, J=1.2 Hz, 1H), 7.18-7.31 (m, 2H), 7.33-7.47 (m, 3H); ESIMS found for C₁₂H₉FN₂O₂ m/z 233 (M+H).

Step 2

To a solution of 2′-fluoro-2-nitrobiphenyl-3-amine (LXVIII) (1.0 g, 3.45 mmol, 1 eq) in MeOH (50 mL) was added Pd/C (0.5 g) under nitrogen atmosphere, the mixture was stirred under 50 psi of H₂ for 6 h at room temperature. Then the mixture was filtered and concentrated in vacuo to afford 2′-fluorobiphenyl-2,3-diamine (LXIX) (0.8 g, 3.96 mmol, 92% yield) as a black solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.99 (s, 2H), 4.62 (s, 2H), 6.32 (d, J=7.6 Hz, 1H), 6.49 (t, J=7.6 Hz, 1H), 6.60 (d, J=7.6 Hz, 1H), 7.21-7.35 (m, 3H), 7.35-7.45 (m, 1H); ESIMS found for C₁₂H₁₁FN₂ m/z 203 (M+H).

The following intermediates were prepared in accordance with the procedure described in the above Scheme 14.

3′-Fluorobiphenyl-2,3-diamine (LXX): White solid (2.0 g, 9.89 mmol, 81% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.16 (s, 2H), 4.64 (s, 2H), 6.38 (dd, J=7.6 Hz, J=1.6 Hz, 1H), 6.51 (t, J=7.6 Hz, 1H), 6.60 (d, J=6 Hz, 1H), 7.11-7.26 (m, 3H), 7.48 (q, J=6.4 Hz, 1H); ESIMS found for C₁₂H₁₁FN₂ m/z 203 (M+H).

4′-Fluorobiphenyl-2,3-diamine (LXXI): White solid (2.4 g, 11.87 mmol, 98% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.07 (s, 2H), 4.60 (s, 2H), 6.34 (dd, J=7.6 Hz, J=1.6 Hz, 1H), 6.50 (t, J=7.6 Hz, 1H), 6.58 (dd, J=7.6 Hz, J=1.6 Hz, 1H), 7.26 (t, J=7.6 Hz, 2H), 7.40 (q, J=5.6 Hz, 2H); ESIMS found for C₁₂H₁₁FN₂ m/z 203 (M+H).

3-(Pyridin-3-yl)benzene-1,2-diamine (LXXII): White solid (1.36 g, 7.34 mmol, 92.5% yield). ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.57 (brs, 2H), 3.42 (brs, 2H), 6.66 (dd, J=6 Hz, J=3.2 Hz, 1H), 6.68-6.72 (m, 2H), 7.31 (dd, J=8 Hz, J=4.8 Hz, 1H), 7.71 (td, J=8 Hz, J=2 Hz, 1H), 8.54 (dd, J=4.8 Hz, J=1.6 Hz, 1H), 8.64 (d, J=1.6 Hz, 1H); ESIMS found for C₁₁H₁₁N₃ m/z 186 (M+H).

3-(Thiophen-3-yl)benzene-1,2-diamine (LXXIII): White solid (1.2 g, 6.31 mmol, mmol, 94% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.19 (s, 2H), 4.59 (s, 2H), 6.47 (dd, J=4.8 Hz, J=1 Hz, 2H), 6.55 (q, J=4.8 Hz, 1H), 7.24 (dd, J=4.8 Hz, J=1 Hz, 1H), 7.50 (t, J=1.6 Hz, 1H), 7.63 (dd, J=4.8 Hz, J=2.8 Hz, 1H); ESIMS found for C₁₀H₁₀N₂S m/z 191 (M+H).

3-(Furan-3-yl)benzene-1,2-diamine (LXXIV): White solid (1.3 g, 7.46 mmol, mmol, 85% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.24 (brs, 2H), 4.57 (brs, 2H), 6.46-6.50 (m, 1H), 6.50-6.56 (m, 2H), 6.72 (s, 1H), 7.74 (t, J=1.6 Hz, 1H), 7.87 (s, 1H); ESIMS found for C₁₀H₁₀N₂O m/z 175 (M+H).

3-(Thiophen-2-yl)benzene-1,2-diamine (LXXV): Brown oil (925.5 mg, 4.86 mmol, 60.9% yield). ESIMS found C₁₀H₁₀N₂S m/z 191.1 (M+H).

1-(5-(2,3-Diaminophenyl)thiophen-2-yl)ethan-1-one (LXXVI): Brown oil (530 mg, 2.28 mmol, 88.1% yield). ¹H NMR (CDC₃, 400 MHz) δ ppm 2.59 (s, 3H), 3.47 (brs, 2H), 3.86 (brs, 2H), 6.70-6.81 (m, 2H), 6.88 (dd, J=2 Hz, J=7.6 Hz, 1H), 7.22 (d, J=3.6 Hz, 1H), 7.71 (d, J=4 Hz, 1H); ESIMS found C₁₂H₁₂N₂OS m/z 233.0 (M+H).

2′,3′-Diamino-5-fluoro-[1,1′-biphenyl]-3-ol (LXXVII): Black oil (165 mg, 0.756 mmol, 83.0% yield). ESIMS found for C₁₂H₁₁FN₂O m/z 219.1 (M+H).

3′-Fluoro-5′-methoxy-[1,1′-biphenyl]-2,3-diamine (LXXVIII): Black oil (187 mg, 0.805 mmol, 93.4% yield). ESIMS found for C₁₃H₁₃FN₂O m/z 233.1 (M+H).

3′-Fluoro-5′-(2-(pyrrolidin-1-yl)ethoxy)-[1,1′-biphenyl]-2,3-diamine (LXXIX): Black oil (300 mg, 0.951 mmol, 68.4% yield). ESIMS found for C₁₈H₂₂FN₃O m/z 316.1 (M+H).

3′-(2-(Dimethylamino)ethoxy)-5′-fluoro-[1,1′-biphenyl]-2,3-diamine (LXXX): Black oil (267 mg, 0.923 mmol, 63.2% yield). ESIMS found for C₁₆H₂₀FN₃O m/z 290.1 (M+H).

Preparation of intermediate 3-(pyridin-4-yl)benzene-1,2-diamine (LXXXVI) is depicted below in Scheme 15.

Step 1

To a solution of 2-bromoaniline (LXXXI) (50 g, 0.29 mol, 1 eq) in acetic anhydride (265 mL) was added dropwise nitric acid (fuming) (36.75 mL, 0.93 mol, 3.2 eq) at 0° C. and then stirred at that temperature, when the starting material was consumed, the mixture was filtered, the filtrate was poured into ice water. The aqueous phase was basified with aqueous solution of sodium bicarbonate to pH=7, then the mixture was extracted with EtOAc (30 mL×3). The organic layers were combined, dried and concentrated in vacuo to give the N-(2-bromo-6-nitrophenyl)acetamide (LXXXII) (12.6 g, 48.6 mmol, 16.7% yield) as a white solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.06 (s, 3H), 7.43 (t, J=8 Hz, 1H), 7.94 (d, J=8 Hz, 1H), 8.05 (d, J=8 Hz, 1H); ESIMS found for C₈H₇BrN₂O₃ m/z 259 (M+H).

Step 2

A degassed mixture of N-(2-bromo-6-nitrophenyl)acetamide (LXXXII) (2.59 g, 10 mmol, 1.0 eq), 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridine (LXXXIII) (2.05 g, 10 mmol, 1.3 eq), Na₂CO₃ (2.12 g, 20 mmol, 2 eq) and Pd(PPh₃)₄ (1.16 g, 1 mmol, 0.1 eq) in a mixed solvent of DME (30 mL) and H₂O (10 mL) was heated to reflux under nitrogen overnight, the mixture was poured onto water (40 ml) and extracted with EtOAc (30 mL×3). The combined organic layers were dried over Na₂SO₄, filtered and concentrated in vacuo, purification the resultant residue was purified by column chromatography (EtOAc:PE=1:4→100% EtOAc) to afford N-(2-nitro-6-(pyridin-4-yl)phenyl)acetamide (LXXXIV) (1.42 g, 5.52 mmol, 55% yield) as a yellow solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.92 (s, 3H), 7.46 (d, J=5.6 Hz, 2H), 7.69 (t, J=8 Hz, 1H), 7.80 (dd, J=7.6 Hz, J=1.2 Hz, 1H), 8.06 (dd, J=8 Hz, J=1.6 Hz, 1H), 8.73 (d, J=6 Hz, 2H), 9.96 (s, 1H); ESIMS found for C₁₃H₁₁N₃O₃ m/z 258 (M+H).

Step 3

To a solution of N-(2-nitro-6-(pyridin-4-yl)phenyl)acetamide (LXXXIV) (3.94 g, 15 mmol, 1 eq) in MeOH (20 mL) was added 2 N aqueous NaOH solution (50 mL) and the mixture was refluxed until the starting material was consumed completely, the precipitate was collected by filtration to afford the 2-nitro-6-(pyridin-4-yl)aniline (LXXXV) (3.0 g, 13.9 mmol, 91% yield) as yellow solid. ESIMS found for C₁₁H₉N₃O₂ m/z 216 (M+H).

Step 4

To a solution of 2-nitro-6-(pyridin-4-yl)aniline (LXXXV) (3 g, 14 mmol, 1 eq) in EtOAc (350 mL) was added Pd/C (0.3 g) and the mixture was stirred at room temperature under 1 atm of H₂ atmosphere overnight, the mixture was filtered and concentrated in vacuo to give the product 3-(pyridin-4-yl)benzene-1,2-diamine (LXXXVI) (2.4 g, 13.0 mmol, 93% yield) as a white solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.35 (s, 2H), 4.75 (s, 2H), 6.45 (dd, J=7.6 Hz, J=1 Hz, 1H), 6.58 (t, J=7.6 Hz, 1H), 6.67 (d, J=6.8 Hz, 1H), 7.47 (d, J=6 Hz, 2H), 8.65 (d, J=6 Hz, 2H); ESIMS found for C₁₁H₁₁N₃ m/z 186 (M+H).

Preparation of intermediate 3-(pyridin-2-yl)benzene-1,2-diamine 3HCl (XCI) is depicted below in Scheme 16.

Step 1

To a solution of 2-bromopyridine (LXXXVII) (10 g, 63 mmol, 1.00 eq) in THF (150 mL) was added n-BuLi (25.3 mL, 63 mmol, 1.00 eq) and the mixture was stirred at −70° C. for 30 min under nitrogen atmosphere. Then n-Bu₃SnCl (21.7 g, 67 mmol, 1.06 eq) was added and the mixture was stirred at the same temperature for another 2 h. Saturated ammonium chloride solution (150 mL) was added to the solution and extracted with EtOAc (150 mL×3). The combined organic layers were dried over Na₂SO₄, filtered and concentrated in vacuo to afford the crude 2-(tributylstannyl)pyridine (LXXXVIII) (25.9 g, 63 mmol, 100% yield) as a yellow oil. The crude product was used without further purification.

Step 2

A degassed mixture of N-(2-bromo-6-nitrophenyl)acetamide (LXXXII) (4.8 g, 19 mmol, 1.00 eq), 2-(tributylstannyl)pyridine (LXXXVIII) (7.5 g, 20 mmol, 1.05 eq) and Pd(PPh₃)₄ (2.1 g, 1.8 mmol, 0.01 eq) in toluene (60 mL) was heated to reflux under nitrogen overnight. Saturated sodium bicarbonate solution (50 mL) was then added to the mixture and it was extracted with EtOAc (50 mL×3). The combined organic layers were dried over Na₂SO₄, filtered and concentrated in vacuo, the residue was purified by column chromatography on silica gel (EtOAc:PE=1:2-100% EtOAc) to afford N-(2-nitro-6-(pyridin-2-yl)phenyl)acetamide (LXXXIX) (4.4 g, 17.1 mmol, 92% yield) as a white-off solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.93 (s, 3H), 7.43-7.51 (m, 1H), 7.51-7.65 (m, 1H), 7.67 (d, J=7.6 Hz, 1H), 7.97 (dd, J=7.6 Hz, J=2.4 Hz, 3H), 8.75 (d, J=4.4 Hz, 1H), 10.52 (s, 1H); ESIMS found for C₁₃H₁₁N₃O₃ m/z 258.1 (M+H).

Step 3

To a solution of N-(2-nitro-6-(pyridin-2-yl)phenyl)acetamide (LXXXIX) (4.41 g, 17 mmol, 1 eq) in MeOH (20 mL) was added 2N NaOH aqueous (50 mL) and the mixture was refluxed until the stirring material was consumed completely. The mixture was concentrated in vacuo to remove the MeOH and the precipitate was collected by filtration to afford 2-nitro-6-(pyridin-2-yl)aniline (XC) (2.4 g, 11.2 mmol, 65% yield) as a yellow solid. ESIMS found for C₁₁H₉N₃O₂ m/z 216.1 (M+H).

Step 4

To a solution of 2-nitro-6-(pyridin-2-yl)aniline (XC) (2.4 g, 0.01 mmol, 1 eq) in EtOAc (350 mL) was added Pd/C (1 g) and the mixture was stirred at room temperature under 1 atm of H₂ atmosphere overnight, filtered and then concentrated in vacuo, to give 3-(pyridin-2-yl)benzene-1,2-diamine (1.9 g, 10.3 mmol, 89% yield) as a yellow oil. ESIMS found for C₁₁H₁₁N₃ m/z 186.0 (M+H).

Step 5

To a solution of 3-(pyridin-2-yl)benzene-1,2-diamine (1.86 g, 0.01 mmol) in EtOAc (200 mL) was added HCl in EtOAc (40 mL) and the mixture was stirred at 0° C. for 20 min. The precipitate was collected by filtration to give 3-(pyridin-2-yl)benzene-1,2-diamine-3HCl (XCI) as a yellow solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 6.89 (t, J=7.6 Hz, 1H), 7.33 (brs, 1H), 7.51 (d, J=7.2 Hz, 1H), 7.54-7.66 (m, 2H), 7.97 (d, J=8 Hz, 1H), 8.16 (brs, 1H), 8.75 (brs, 1H).

Preparation of intermediate 3-(piperidin-1-yl)benzene-1,2-diamine (XCIV) is depicted below in Scheme 17.

Step 1

To a solution of 3-chloro-2-nitroaniline (XCII) (2.00 g, 11.6 mmol, 1 eq) and piperidine (2.95 g, 34.7 mmol, 3 eq) in DMF (60 ml) was added K₂CO₃ (4.78 g, 34.4 mmol, 3 eq) in one portion and the mixture was stirred at 120° C. under nitrogen overnight. The reaction mixture was diluted with EtOAc (60 ml) and washed with saturated NaHCO₃ solution (50 mL). The organic phases were dried over Na₂SO₄ and concentrated in vacuo, the resultant residue was purified by silica gel column chromatography (PE:EtOAc=5:1-1:1) to give 2-nitro-3-(piperidin-1-yl)aniline (XCIII) (1.8 g, 8.14 mmol, 70.3% yield) as a black solid. ESIMS found for C₁₁H₁₅N₃O₂ m/z 222 (M+H).

Step 2

A mixture of 2-nitro-3-(piperidin-1-yl)aniline (XCIII) (1.64 g, 6.9 mmol, 1 eq) and Pd/C (0.50 g) in MeOH (20 mL) was stirred at room temperature under 30 psi H₂ overnight. After the starting material was consumed completely, the mixture was filtered through a Celite pad and the filtrate was concentrated in vacuo to give the 3-(piperidin-1-yl)benzene-1,2-diamine (XCIV) (1.1 g, 5.75 mmol, 76% yield) as a yellow solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.59 (brs, 2H), 1.73 (quin, J=5.6 Hz, 4H), 2.84 (brs, 4H), 3.50 (brs, 4H), 6.52 (dd, J=6.4 Hz, J=1.6 Hz, 1H), 6.59-6.75 (m, 2H); ESIMS found for C₁₁H₁₇N₃ m/z 192 (M+H).

Preparation of intermediate 3-(4-methyl-imidazol-1-yl)-benzene-1,2-diamine (XCVI) is depicted below in Scheme 18.

Step 1

A solution of 3-chloro-2-nitro-aniline (XCII) (1.0 g, 5.8 mmol), potassium carbonate (2.4 g, 17.4 mmol), and 4-methylimidazole in dry DMF was heated overnight at 120° C. under nitrogen. The reaction was cooled and the solvent was evaporated in vacuo. The residue was suspended in a saturated NaHCO₃ solution and extracted with CH₂Cl₂. The combined organic phases were dried over MgSO₄ and concentrated in vacuo. The crude product was purified by flash chromatography to provide 3-(4-methyl-imidazol-1-yl)-2-nitro-phenylamine (XCV). ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.19 (s, 3H), 6.53 (m, 1H), 6.79 (m, 1H), 6.93 (m, 1H), 7.32 (m, 1H), 7.60 (m, 1H).

Step 2

To a solution of 3-(4-methyl-imidazol-1-yl)-2-nitro-phenylamine (XCV) in MeOH was added with 5% Pd/C. The combination was stirred under a hydrogen filled balloon at 40° C. for 6 h. The solution was then filtered through a pad of Celite. The filtrate was concentrated in vacuo to get 3-(4-methyl-imidazol-1-yl)-benzene-1,2-diamine (XCVI). ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.17 (s, 3H), 6.54 (m, 1H), 6.80 (m, 1H), 6.97 (m, 1H), 7.28 (m, 1H), 7.56 (m, 1H).

Preparation of intermediate (XCIX) is depicted below in Scheme 19.

Step 1

A mixture of 1-methylpiperazine (XCVII) (20 mL) and 3-chloro-2-nitroaniline (XCII) (1.5 g, 8.7 mmol, 1 eq) was stirred at 50° C. for 1 h under microwave irradiation. The reaction mixture was diluted with water (100 mL) and filtered, the cake washed with water (30 mL×3), dried in vacuo to give the 3-(4-methylpiperazin-1-yl)-2-nitroaniline (XCVIII) (1.64 g, 6.94 mmol, 80% yield) as a yellow solid. ESIMS found for C₁₁H₁₆N₄O₂ m/z 237 (M+H).

Step 2

A mixture of 3-(4-methylpiperazin-1-yl)-2-nitroaniline (XCVIII) (1.64 g, 6.9 mmol, 1 eq) and Pd/C (0.2 g) in MeOH (20 mL) was stirred under 30 psi of H₂ at room temperature overnight. The reaction was monitored by TLC. The mixture was filtered and the filtrate was concentrated in vacuo to give the 3-(4-methylpiperazin-1-yl)benzene-1,2-diamine (XCIX) (1.31 g, 6.35 mmol, 92% yield) as a black solid. ¹H NMR (CDCl₃, 400 MHz) δ ppm 2.30 (s, 3H), 3.30 (brs, 2H), 3.68 (brs, 2H), 6.46 (dd, J=7.2 Hz, J=2 Hz, 1H), 6.54-6.63 (m, 2H); ESIMS found for C₁₁H₁₈N₄ m/z 207 (M+H).

Preparation of intermediate 3-(5-fluorothiophen-2-yl)benzene-1,2-diamine (CIII) is depicted below in Scheme 20.

Step 1

A solution of 2-bromo-6-nitroaniline (C) (800 mg, 3.67 mmol, 1.0 eq), 2-(5-fluorothiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CI) (1.0 g, 4.4 mmol, 1.2 eq), Pd(dppf)Cl₂ (268 mg, 0.37 mmol, 0.10 eq) and Cs₂CO₃ (2.39 g, 7.34 mmol, 2.0 eq) in dioxane (20 mL) and H₂O (4 mL) was de-gassed and then heated to 100° C. under N₂ for 3 h. TLC (PE:EtOAc=1:1) showed the starting material was consumed completely. The mixture was concentrated in vacuum to give a residue, which was purified by column chromatography (PE:EtOAc=10:1) to afford the 2-(5-fluorothiophen-2-yl)-6-nitroaniline (CII) (540 mg, 2.27 mmol, 61.7% yield). ESIMS found for C₁₀H₇FN₂O₂S m/z 239.1 (M+H).

Step 2

To a mixture of 2-(5-fluorothiophen-2-yl)-6-nitroaniline (CII) (500 mg, 2.09 mmol) in EtOAc (20 mL) was added Zn power (410 mg, 6.27 mmol) and cooled to 0-5° C. HOAc (3 mL) was then added dropwise and the mixture was stirred at 15-20° C. for 1 h. LC/MS showed the starting material was consumed completely. The mixture was filtered through Celite®, washed with EtOAc (5×50 mL), the organic phase was washed with NaHCO₃ (50 mL×2), brine (50 mL), water, then concentrated to dryness to afford 3-(5-fluorothiophen-2-yl)benzene-1,2-diamine (CIII) (400 mg, 1.92 mmol, 91.9% yield). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.42 (brs, 2H), 4.70 (brs, 2H), 6.45 (s, 1H), 6.46 (s, 1H), 6.56 (t, J=4.4 Hz, 1H), 6.71 (dd, J=2.8 Hz, J=4 Hz, 1H), 6.83 (t, J=4 Hz, 1H); ESIMS found for C₁₀H₉FN₂S m/z 209.0 (M+H).

Preparation of intermediate 3-(5-methylthiophen-2-yl)benzene-1,2-diamine (CVI) is depicted below in Scheme 21.

Step 1

A solution of 3-bromo-2-nitroaniline (LXVI) (1.80 g, 8.29 mmol, 1 eq), 4,4,5,5-tetramethyl-2-(5-methylthiophen-2-yl)-1,3,2-dioxaborolane (CIV) (2.23 g, 9.95 mmol, 1.2 eq), Na₂CO₃ (3.08 g, 29.01 mmol, 3.5 eq) and Pd(dppf)Cl₂ (307.47 mg, 414.50 μmol, 0.05 eq) in dioxane (30 mL) and water (6 mL) was de-gassed and then heated to 80° C. overnight under N₂. TLC (PE:EtOAc=1:1) showed the starting material was consumed completely. The reaction mixture was poured into H₂O (300 mL). The mixture was extracted with EtOAc (3×250 mL). The organic phase was washed with saturated brine (300 mL), dried over anhydrous Na₂SO₄, concentrated in vacuum to give a residue. The crude product was purified by silica gel chromatography (PE:EtOAc=10:1) to give 3-(5-methylthiophen-2-yl)-2-nitroaniline (CV) (1.20 g, 5.12 mmol, 61.79% yield) as a brown solid. ESIMS found for C₁₁H₁₀N₂O₂S m/z 345.1 (M+H).

Step 2

To a solution of 3-(5-methylthiophen-2-yl)-2-nitroaniline (CV) (1.20 g, 5.12 mmol, 1 eq) in MeOH (30 mL) and H₂O (10 mL) was added Fe (1.14 g, 20.48 mmol, 4.0 eq) and NH₄Cl (2.20 g, 40.96 mmol, 8.0 eq) in one portion at room temperature. The mixture was stirred at room temperature for 10 min. Then heated to 80° C. and stirred for 16 hours. TLC showed the reaction was completed. The mixture was cooled to room temperature and concentrated under reduced pressure at 60° C. After filtration, the aqueous phase was extracted with EtOAc (400 mL×3). The combined organic phase was washed with saturated brine (200 mL×2), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum to produce 3-(5-methylthiophen-2-yl)benzene-1,2-diamine (CVI) (1.00 g, 4.90 mmol, 95.61% yield) as a brown solid. ¹H NMR (CD₃OD, 400 MHz) δ ppm 2.51 (s, 3H), 6.62 (t, J=4 Hz, 1H), 6.66-6.75 (m, 2H), 6.79 (s, 1H), 6.93 (d, J=3.2 Hz, 1H); ESIMS found for C₁₁H₁₂N₂S m/z 205.0 (M+H).

Preparation of intermediate N-((2′,3′-diamino-5-fluoro-[1,1′-biphenyl]-3-yl)methyl)methanesulfonamide (CXII) is depicted below in Scheme 22.

Step 1

A solution of 3-bromo-5-fluorobenzonitrile (CVII) (44.0 g, 220.0 mmol, 1.0 eq) was dissolved in THF (30 mL). BH₃-Me₂S (33.43 g, 440.0 mmol, 2.0 eq) was added to the solution at 20° C. Then it was stirred at 80° C. for 2 h, HCl (6 N, 100 mL) was added to the mixture slowly at 20° C. The mixture was stirred at 80° C. for 1 h, then it was washed with EtOAc (300 ml). The water phase was basified with 50% aqueous NaOH and it was extracted with EtOAc (300 mL×3). The combined organic layers were dried over anhydrous Na₂SO₄ and concentrated in vacuo to produce (3-bromo-5-fluoro-phenyl)methanamine (CVIII) (24.0 g, 117.62 mmol, 53.5% yield). ¹H NMR (CDCl₃, 300 MHz) 3.86 (s, 2H), 7.01 (d, J=8 Hz, 1H), 7.12 (d, J=8 Hz, 1H), 7.28 (s, 1H); ESIMS found C₇H₇BrFN m/z 203.9 (Br⁷⁹M+H).

Step 2

A solution of (3-bromo-5-fluoro-phenyl)methanamine (CVIII) (23.0 g, 112.7 mmol, 1.0 eq) was dissolved in DCM (15 mL), TEA (34.22 g, 338.2 mmol, 3.0 eq) was added to the mixture. Then MsC1 (13.44 g, 117.3 mmol, 1.04 eq) was added slowly to the solution at 0° C. It was stirred at 0-30° C. for 2 h. The reaction was washed with water and extracted with EtOAc. The combined organic layers were dried over anhydrous Na₂SO₄ and concentrated to give N-(3-bromo-5-fluorobenzyl) methanesulfonamide (CIX) (34.0 g, 102.44 mmol, 90.9% yield, 85% purity) as an oil. ¹H NMR (CDC₃, 300 MHz) 2.88 (s, 3H), 4.24 (d, J=4.5 Hz, 2H), 6.99 (d, J=9 Hz, 1H), 7.13 (dt, J=8.1 Hz, J=2 Hz, 1H), 7.25 (s, 1H); ESIMS found C₈H₉BrFNO₂S m/z 282.0 (Br⁷⁹M+H).

Step 3

A solution of N-(3-bromo-5-fluorobenzyl)methanesulfonamide (CIX) (34.0 g, 102.4 mmol, 1.0 eq) and 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,3,2-dioxaborolane (52.02 g, 204.9 mmol, 2.0 eq), KOAc (20.11 g, 204.9 mmol, 2.0 eq) was dissolved in dioxane (20 mL). Then Pd(dppf)Cl₂ (7.60 g, 10.2 mmol, 0.1 eq) was added to the mixture. It was stirred at 90° C. for 2 h. Then the solvent was removed to get the residue which was purified by silica gel column (PE:EtOAc=10:1→100% EtOAc) to get N-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)methanesulfonamide (CX) (30.0 g, crude). ¹H NMR (CDCl₃, 400 MHz) 1.37 (s, 12H), 2.92 (s, 3H), 4.34 (d, J=6.3 Hz, 2H), 7.19 (dt, J=9.3 Hz, J=2.1 Hz, 1H), 7.44 (dd, J=8.7 Hz, J=2.4 Hz, 1H), 7.54 (s, 1H); ESIMS found C₁₄H₂₁BFNO₄S m/z 330.1 (M+H).

Step 4

A solution of 2-bromo-6-nitroaniline (C) (1.80 g, 8.29 mmol, 1 eq), N-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl) methanesulfonamide (CX) (3.27 g, 9.95 mmol, 1.2 eq), Na₂CO₃ (3.08 g, 29.01 mmol, 3.5 eq) and Pd(dppf)Cl₂ (307.47 mg, 414.50 mol, 0.05 eq) in dioxane (30 mL) and water (6 mL) was de-gassed and then heated to 80° C. overnight under N₂. TLC (PE:EtOAc=1:1) showed the starting material was consumed completely. The reaction mixture was poured into H₂O (300 mL). The mixture was extracted with EtOAc (3×250 mL). The organic phase was washed with saturated brine (300 mL), dried over anhydrous Na₂SO₄, concentrated in vacuum to give a residue. The crude product was purified by silica gel chromatography (PE:EtOAc=10:1) to give N-((2′-amino-5-fluoro-3′-nitro-[1,1′-biphenyl]-3-yl)methyl)methanesulfonamide (CXI) (1.60 g, 4.72 mmol, 56.9% yield) as a brown solid. ESIMS found for C₁₄H₁₄FN₃O₄S m/z 340.1 (M+H).

Step 5

To a solution of N-((2′-amino-5-fluoro-3′-nitro-[1,1′-biphenyl]-3-yl)methyl)methanesulfonamide (CXI) (1.60 g, 4.72 mmol, 1 eq) in MeOH (30 mL) was added Fe (1.05 g, 18.88 mmol, 4.0 eq) and NH₄Cl (2.02 g, 37.76 mmol, 8.0 eq) in one portion at room temperature. The mixture was stirred at room temperature for 10 min. Then heated to 80° C. and stirred for 16 hours. TLC showed the reaction was completed. The mixture was cooled to room temperature and concentrated under reduced pressure at 60° C. After filtration, the aqueous phase was extracted with EtOAc (400 mL×3). The combined organic phase was washed with saturated brine (100 mL×2), dried with anhydrous Na₂SO₄, filtered and concentrated in vacuum to produce N-((2′,3′-diamino-5-fluoro-[1,1′-biphenyl]-3-yl)methyl)methanesulfonamide (CXII) ((1.20 g, 3.88 mmol, 82.2% yield) as a brown solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.92 (s, 3H), 4.23 (d, J=6 Hz, 2H), 4.97 (brs, 4H), 6.44 (d, J=7.2 Hz, 1H), 6.54 (t, J=7.6 Hz, 1H), 6.66 (d, J=7.6 Hz, 1H), 7.11 (dd, J=9.8 Hz, J=18.4 Hz, 2H), 7.22 (s, 1H), 7.68 (d, J=4.8 Hz, 1H); ESIMS found for C₁₄H₁₆FN₃O₂S m/z 310.1 (M+H).

Preparation of intermediate N^(3′)-(2-(dimethylamino)ethyl)-5′-fluoro-[1,1′-biphenyl]-2,3,3′-triamine (CXVII) is depicted below in Scheme 23.

Step 1

A solution of 3-bromo-5-fluorobenzaldehyde (CXIII) (20.0 g, 98.2 mmol, 1.0 eq) in MeOH (1.8 L) was added N¹,N¹-dimethylethane-1,2-diamine (21.5 mL, 196.4 mmol, 2.0 eq). The pH was adjusted to 6 using HOAc and stirred for 1 h. NaCNBH₃ (8.6 g, 137.5 mmol, 1.4 eq) was added and stirred at room temperature overnight. The MeOH was removed under vacuum and the residue was partitioned between CHCl₃ and saturated aqueous NaHCO₃. The organic layer was dried over MgSO₄ and evaporated under vacuum. The crude product was purified on a silica gel column (100% CHCl₃→3:97 MeOH[7N NH₃]:CHCl₃) to produce N¹-(3-bromo-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (CXIV) as a yellow oil (13.0 g, 49.9 mmol, 51% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.28 (s, 6H), 2.39 (t, J=4 Hz, 2H), 3.07 (q, J=6 Hz, 2H), 6.10 (t, J=5 Hz, 1H), 6.38 (td, J=12 Hz, J=2 Hz, 1H), 6.51 (td, J=8.6 Hz, J=2 Hz, 1H), 6.61 (t, J=2 Hz, 1H); ESIMS found C₁₀H₁₄BrFN₂ m/z 261.0 (M+H).

Step 2

A solution of N¹-(3-bromo-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (CXIV) (13.0 g, 49.9 mmol, 1.0 eq), bis(pinacolato)diboron (12.6 g, 59.9 mmol, 1.2 eq), KOAc (12.1 g, 124.3 mmol, 2.5 eq) and dioxane (600 mL) was purged with argon. Pd(dppf)Cl₂ (2.0 g, 2.47 mmol, 0.05 eq) was added to the reaction and purged again with argon. The solution was heated at 90° C. for 2 h. Once TLC showed the disappearance of (CXIV), the solution was cooled to room temperature and then concentrated under reduced pressure to produce crude N¹-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (CXV) (7.4 g, 24.0 mmol, 48.2% yield).

Step 3

To a solution of N¹-(3-fluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (CXV) (4.00 g, 12.98 mmol, 1.0 eq) in dioxane (80 mL) and water (0.5 mL) was added K₂CO₃ (3.60 g, 26.0 mmol, 2.0 eq), 3-bromo-2-nitroaniline (LXVI) (2.82 g, 12.98 mmol, 1.0 eq) and Pd(dppf)Cl₂ (481.42 mg, 649.0 μmol, 0.05 eq). The solution was purged with argon and heated at 80° C. for 4 h. The solution was cooled to room temperature and then concentrated under reduced pressure to give N^(3′)-(2-(dimethylamino)ethyl)-5′-fluoro-2-nitro-[1,1′-biphenyl]-3,3′-diamine (CXVI) as a solid (2.5 g, 7.86 mmol, 60.5% yield). ESIMS found for C₁₆H₁₉FN₄O₂ m/z 319.1 (M+H).

Step 4

To a solution of N^(3′)-(2-(dimethylamino)ethyl)-5′-fluoro-2-nitro-[1,1′-biphenyl]-3,3′-diamine (CXVI) (2.50 g, 7.86 mmol, 1.0 eq) in MeOH (80 mL) was added Pd/C under N₂. The suspension was degassed under vacuum and purged with H₂ several times. The mixture was stirred under H₂ (25 psi) at room temperature for 24 h. LC/MS showed the starting material was consumed completely. The reaction mixture was filtered and the filter was concentrated. The crude product was purified by silica gel chromatography (MeOH:DCM=10:1) to give N^(3′)-(2-(dimethylamino)ethyl)-5′-fluoro-[1,1′-biphenyl]-2,3,3′-triamine (CXVII) (2.0 g, 6.94 mmol, 88.2% yield) as yellow solid. ¹H NMR (CD₃OD, 400 MHz) δ ppm 2.32 (s, 6H), 2.60 (t, J=6.8 Hz, 2H), 3.26 (t, J=6.8 Hz, 2H), 6.31-6.38 (m, 2H), 6.45 (t, J=1.6 Hz, 1H), 6.59 (dd, J=1.6 Hz, J=7.6 Hz, 1H), 6.66 (t, J=7.6 Hz, 1H), 7.73 (dd, J=1.6 Hz, J=7.6 Hz, 1H); ESIMS found C₁₆H₂₁FN₄ m/z 289.1 (M+H).

Preparation of N,N-dimethyl-1-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (169) is depicted below in Scheme 24.

Step 1

A solution of 1-(5-bromopyridin-3-yl)-N,N-dimethylmethanamine (XXXIX) (10.00 g, 46.49 mmol, 1.0 eq), Pd(dppf)Cl₂ (1.70 g, 2.32 mmol, 0.05 Eq), KOAc (9.1 g, 93.0 mmol, 2.0 eq) and Pin₂B₂ (11.75 g, 46.49 mmol, 1.0 eq) in dioxane (230 mL) was de-gassed and then heated to 110° C. under N₂ for 12 hr. TLC (PE:EtOAc=2:1) showed the starting material was consumed completely. The reaction mixture was filtered and concentrated to afford the crude (5-((dimethylamino)methyl)pyridin-3-yl)boronic acid (CXVIII). The crude product was used in the next step without purification.

Step 2

To a solution of tert-butyl 5-bromo-3-(diethoxymethyl)-1H-pyrazolo [3,4-c]pyridine-1-carboxylate (XVI) (3.0 g, 7.49 mmol, 1.0 eq) and (5-((dimethylamino)methyl)pyridin-3-yl)boronic acid (CXVIII) (2.02 g, 11.24 mmol, 1.5 eq) in dioxane (30 mL) and H₂O (5 mL) was added Pd(dppf)Cl₂ (111.1 mg, 149.9 μmol, 0.02 eq), K₂CO₃ (2.07 g, 15.0 mmol, 2.0 eq) at room temperature. The mixture was stirred at 90° C. for 3 hr. TLC (PE:EtOAc=0:1) showed that the starting material was consumed completely. The mixture was added water (50 mL) and extracted with EtOAc (80 mL×2). The organic layers were washed with brine (60 mL), concentrated. The residue was purified by chromatography on silica gel (PE:EtOAc=1:1-1:5) to afford tert-butyl 3-(diethoxymethyl)-5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c] pyridine-1-carboxylate (CXIX) (0.84 g, 1.84 mmol, 24.6% yield) as a brown oil. ¹H NMR (CDCl₃, 400 MHz) δ ppm 1.31 (t, J=7.2 Hz, 6H), 1.79 (s, 9H), 3.57 (s, 2H), 3.64-3.75 (m, 2H), 3.80-3.93 (m, 2H), 5.86 (s, 1H), 8.35 (s, 1H), 8.38 (d, J=1.6 Hz, 1H), 8.60 (d, J=1.6 Hz, 1H), 9.19 (d, J=2.4 Hz, 1H), 9.55 (s, 1H); ESIMS found for C₂₄H₃₃N₅O₄ m/z 456.1 (M+H).

Step 3

A mixture of tert-butyl 3-(diethoxymethyl)-5-(5-((dimethylamino) methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine-1-carboxylate (CXIX) (100.0 mg, 0.22 mmol, 1 eq), 3-(piperidin-1-yl)benzene-1,2-diamine (LVII) (42 mg, 0.22 mmol, 1.0 eq) and Na₂S₂O₅ (51 mg, 0.26 mmol, 1.2 eq) in DMF (2 mL) was stirred at 120° C. for 24 h. LC/MS showed CXIX was consumed. Water (5 mL) was added in dropwise and the mixture was filtered. The filtrate was washed by MeOH (0.5 mL) and purified by pre-HPLC (HCl) to give N,N-dimethyl-1-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (169) (16.1 mg, 0.04 mmol, 16.2%) as a white solid. ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.84 (brs, 2H), 2.15 (brs, 4H), 2.80 (d, J=4.02 Hz, 6H), 4.03 (brs, 4H), 4.50 (d, J=4.14 Hz, 2H), 7.41 (t, J=7.92 Hz, 1H), 7.65-7.75 (m, 2H), 8.88 (d, J=1.63 Hz, 1H), 9.07 (s, 1H), 9.16 (s, 1H), 9.37 (d, J=1.13 Hz, 1H), 9.51 (d, J=1.63 Hz, 1H), 10.97 (brs, 1H), 13.91 (brs, 1H), 14.72 (brs, 1H); ESIMS found for C₂₆H₂₈N₈ m/z 453.2 (M+1).

The following compound was prepared in accordance with the procedure described in the above Example 1.

N-(5-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide 1.

White solid (18.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.15 (t, J=7.59 Hz, 3H), 2.48 (q, J=7.68 Hz, 4H), 7.25 (td, J=8.13, 2.32 Hz, 1H), 7.40 (t, J=7.68 Hz, 1H), 7.60-7.70 (m, 3H), 8.15-8.22 (m, 1H), 8.26-8.35 (m, 1H), 9.05 (s, 2H), 9.12 (d, J=3.26 Hz, 2H), 9.36 (s, 1H), 10.80 (brs, 1H), 14.61 (brs, 1H); ESIMS found for C₂₇H₂₀FN₇O m/z 478.1 (M+1).

5-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine 7

White solid (34.3 mg, 0.08 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.07 (s, 6H), 7.27 (td, J=8.12 Hz, J=1.48 Hz, 1H), 7.38 (t, J=7.76 Hz, 1H), 7.54-7.65 (m, 3H), 7.84 (brs, 1H), 8.09-8.17 (m, 1H), 8.20 (d, J=2.38 Hz, 1H), 8.33-8.42 (m, 1H), 8.64 (s, 1H), 8.95 (s, 1H), 9.32 (s, 1H), 13.45 (brs, 1H), 14.39 (s, 1H); ESIMS found for C₂₆H₂₀FN₇ m/z 450.1 (M+1).

1-(5-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine 13

White solid (15.5 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.59 (brs, 6H), 4.03 (brs, 2H), 7.26-7.34 (m, 1H), 7.39 (t, J=7.47 Hz, 1H), 7.57-7.66 (m, 3H), 8.15 (d, J=7.40 Hz, 1H), 8.45-8.52 (m, 1H), 8.52-8.59 (m, 1H), 8.65 (brs, 1H), 9.04 (brs, 1H), 9.33 (brs, 2H), 13.47 (brs, 1H), 14.41 (brs, 1H); ESIMS found for C₂₇H₂₂FN₇ m/z 464.1 (M+1).

N-(5-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide 19

White solid (56.8 mg, 0.12 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.85-0.93 (m, 4H), 1.82-1.91 (m, 1H), 7.25 (td, J=8.34, 2.26 Hz, 1H), 7.39 (t, J=7.64 Hz, 1H), 7.57-7.66 (m, 3H), 8.13-8.23 (m, 1H), 8.33-8.47 (m, 1H), 8.81 (d, J=1.88 Hz, 1H), 8.90 (s, 1H), 8.99 (s, 2H), 9.32 (s, 1H), 10.63 (s, 1H), 13.47 (brs, 1H) 14.40 (s, 1H); ESIMS found for C₂₈H₂₀FN₇O m/z 490.1 (M+1).

5-(5-((3,3-Difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 25

White solid (33.3 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.55-2.65 (m, 2H), 3.78-3.91 (m, 2H), 4.53-4.65 (m, 2H), 7.29 (td, J=8.50, 2.82 Hz, 1H), 7.41 (t, J=7.92 Hz, 1H), 7.59-7.68 (m, 3H), 8.11 (d, J=7.53 Hz, 1H), 8.35-8.44 (m, 1H), 8.94 (brs, 2H), 9.11 (s, 1H), 9.37 (d, J=1.13 Hz, 1H), 9.46 (s, 1H), 14.62 (brs, 1H); ESIMS found for C₂₉H₂₂F₃N₇ m/z 526.1 (M+1).

N-(5-(3-(4-(4-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 28

White solid (13.7 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.99 (d, J=6.53 Hz, 6H), 2.18 (non, J=6.64 Hz, 1H), 2.35 (d, J=7.15 Hz, 2H), 7.39 (t, J=7.76 Hz, 1H), 7.45 (t, J=8.91 Hz, 2H), 7.52 (d, J=6.65 Hz, 1H), 7.58 (d, J=8.28 Hz, 1H), 8.39 (brs, 2H), 8.99 (s, 1H), 9.01-9.07 (m, 1H), 9.15 (brs, 2H), 9.36 (s, 1H), 10.78 (brs, 1H), 14.56 (brs, 1H); ESIMS found for C₂₉H₂₄FN₇O m/z 506.1 (M+1).

N-(5-(3-(4-(4-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide 34

White solid (35.3 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.31 (s, 9H), 7.34-7.45 (m, 3H), 7.49-7.59 (m, 2H), 8.40-8.49 (m, 2H), 8.90-8.95 (m, 3H), 9.03 (d, J=1.63 Hz, 1H), 9.31 (d, J=1.25 Hz, 1H), 9.67 (s, 1H), 13.40 (brs, 1H), 14.37 (s, 1H); ESIMS found for C₂₉H₂₄FN₇O m/z 506.2 (M+1).

3-(4-(4-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 40

White solid (22.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.86-1.97 (m, 2H), 2.03-2.13 (m, 2H), 3.14-3.24 (m, 2H), 3.41-3.52 (m, 2H), 4.61 (d, J=5.77 Hz, 2H), 7.37-7.48 (m, 3H), 7.51 (d, J=6.65 Hz, 1H), 7.62 (d, J=7.91 Hz, 1H), 8.31-8.40 (m, 2H), 8.97 (s, 2H), 9.10 (d, J=1.13 Hz, 1H), 9.36 (d, J=1.25 Hz, 1H), 9.48 (d, J=1.63 Hz, 1H), 10.98 (brs, 1H), 14.62 (brs, 1H); ESIMS found for C₂₉H₂₄FN₇ m/z 490.1 (M+1).

N-(5-(3-(4-(4-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide 46

White solid (45.7 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.80-1.92 (m, 1H), 1.94-2.04 (m, 1H), 2.13-2.25 (m, 2H), 2.27-2.38 (m, 2H), 3.29-3.36 (m, 1H), 7.37 (t, J=7.68, 1H), 7.44 (t, J=8.91 Hz, 2H), 7.52 (d, J=7.15 Hz, 1H), 7.57 (d, J=7.91 Hz, 1H), 8.37-8.48 (m, 2H), 8.80 (s, 1H), 8.94 (s, 1H), 8.99 (s, 1H), 9.03 (d, J=1.88 Hz, 1H), 9.32 (d, J=1.13 Hz, 1H), 10.23 (s, 1H), 13.40 (brs, 1H), 14.38 (s, 1H); ESIMS found for C₂₉H₂₂FN₇O m/z 504.1 (M+1).

3-(4-(4-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine 52

White solid (76.9 mg, 0.19 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.36 (t, J=8.85 Hz, 2H), 7.43-7.52 (m, 2H), 7.67 (dd, J=7.64 Hz, J=1.00 Hz, 1H), 8.16 (dd, J=8.66, 5.65 Hz, 2H), 9.00 (s, 1H), 9.24 (s, 1H), 9.33 (d, J=0.88 Hz, 1H), 9.47 (s, 2H); ESIMS found for C₂₃H₁₄FN₇ m/z 408.0 (M+1).

5-(3-(4-(2-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-amine 55

White solid (31.2 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ 7.39-7.50 (m, 4H), 7.51-7.59 (m, 1H), 7.76-7.84 (m, 1H), 7.86-7.97 (m, 1H), 8.08 (d, J=2.38 Hz, 1H), 8.40 (s, 1H), 8.67 (brs, 1H), 9.04 (br. s., 1H), 9.35 (d, J=1.13 Hz, 1H), 14.82 (brs, 1H); ESIMS found for C₂₄H₁₆FN₇ m/z 422.0 (M+1).

N-(5-(3-(4-(2-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide 61

White solid (43.3 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.18 (d, J=6.78 Hz, 6H), 2.66-2.74 (m, 1H), 7.34-7.53 (m, 5H), 7.57-7.64 (m, 1H), 8.10-8.18 (m, 1H), 8.78 (d, J=3.89 Hz, 1H), 8.84-8.93 (m, 3H), 9.29 (s, 1H), 10.23 (s, 1H), 13.37 (brs 1H), 14.33 (s, 1H); ESIMS found for C₂₈H₂₂FN₇O m/z 492.1 (M+1).

N-(5-(3-(4-(2-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide 68

White solid (7.1 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.08 (s, 9H), 2.31 (s, 2H), 7.35-7.45 (m, 4H), 7.47-7.55 (m, 1H), 7.62-7.73 (m, 1H), 7.94-8.09 (m, 1H), 8.87-8.93 (m, 2H), 8.93-9.02 (m, 2H), 9.32 (d, J=1.13 Hz, 1H), 10.41 (brs, 1H), 14.44 (brs, 1H); ESIMS found for C₃₀H₂₆FN₇O m/z 520.2 (M+1).

N-(5-(3-(4-(2-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide 73

White solid (10.2 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.56-1.66 (m, 2H), 1.66-1.85 (m, 4H), 1.88-1.98 (m, 2H), 2.83-2.93 (m, 1H), 7.32-7.53 (m, 5H), 7.59 (d, J=7.15 Hz, 1H), 8.10-8.18 (m, 1H), 8.76 (s, 1H), 8.83-8.92 (m, 3H), 9.29 (s, 1H), 10.28 (brs, 1H), 13.39 (brs, 1H), 14.35 (brs 1H); ESIMS found for C₃₀H₂₄FN₇O m/z 518.2 (M+1).

5-(Pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 82

White solid (52.5 mg, 0.13 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.52 (t, J=7.72 Hz, 1H), 7.78 (d, J=8.03 Hz, 1H), 7.86 (d, J=7.40 Hz, 1H), 8.20 (dd, J=8.09, 6.09 Hz, 1H), 8.32 (dd, J=7.91, 5.77 Hz, 1H), 8.98 (dd, J=12.92, 5.40 Hz, 2H), 9.15 (s, 1H), 9.24-9.31 (m, 1H), 9.41 (s, 1H), 9.45-9.51 (m, 1H), 9.66 (s, 1H), 10.07 (d, J=0.75 Hz, 1H), 14.76 (brs, 1H); ESIMS found for C₂₃H₁₅N₇ m/z 390.0 (M+1).

N-(5-(3-(4-(Pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide 87

White solid (42.4 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.19 (d, J=6.90 Hz, 6H), 2.65-2.75 (m, 1H), 7.45 (t, J=7.72 Hz, 1H), 7.65-7.72 (m, 2H), 7.88-7.94 (m, 1H), 8.76 (dd, J=5.08, 1.44 Hz, 1H), 8.85 (d, J=2.26 Hz, 1H), 8.93 (d, J=1.13 Hz, 1H), 8.95 (t, J=2.07 Hz, 1H), 9.04 (d, J=2.01 Hz, 1H), 9.08-9.18 (m, 1H), 9.33 (d, J=1.13 Hz, 1H), 9.60 (brs, 1H), 10.30 (s, 1H), 13.56 (brs, 1H) 14.45 (s, 1H); ESIMS found for C₂₇H₂₂N₈O m/z 475.1 (M+1).

N-Isopropyl-5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine 90

White solid (6.3 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.23 (d, J=6.27 Hz, 6H), 3.79 (spt, J=6.04 Hz, 2H), 7.42 (t, J=7.88 Hz, 1H), 7.58-7.66 (m, 3H), 7.87 (brs, 1H), 8.06 (d, J=2.51 Hz, 1H), 8.54 (s, 1H), 8.65 (dd, J=4.77, 1.51 Hz, 1H), 8.72-8.78 (m, 1H), 8.94 (s, 1H), 9.31 (d, J=1.13 Hz, 1H), 9.56 (brs, 1H), 13.49 (brs, 1H), 14.42 (s, 1H); ESIMS found for C₂₆H₂₂N₈ m/z 447.1 (M+1).

5-(5-(Piperidin-1-ylmethyl)pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 93

White solid (49.7 mg, 0.10 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.34-1.48 (m, 1H), 1.66-1.76 (m, 1H), 1.81 (brs, 4H), 2.95-3.08 (m, 2H), 3.46 (d, J=9.54 Hz, 2H), 4.69 (brs, 2H), 7.51 (t, J=7.84 Hz, 1H), 7.78 (d, J=8.03 Hz, 1H), 7.86 (d, J=7.40 Hz, 1H), 8.32 (dd, J=8.28, 5.65 Hz, 1H), 9.02 (d, J=5.40 Hz, 1H), 9.07 (s, 1H), 9.12 (s, 1H), 9.35 (s, 1H), 9.39 (d, J=1.25 Hz, 1H), 9.44 (d, J=8.41 Hz, 1H), 9.61 (d, J=1.63 Hz, 1H), 10.16 (brs, 1H), 11.17 (brs, 1H), 14.84 (brs, 1H); ESIMS found for C₂₉H₂₆N₈ m/z 487.1 (M+1).

N-Benzyl-1-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine 101

White solid (5.3 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.34 (brs, 2H), 4.49-4.54 (m, 2H), 7.42-7.53 (m, 4H), 7.59 (dd, J=7.65, 1.63 Hz, 2H), 7.72-7.84 (m, 3H), 8.12-8.20 (m, 2H), 8.83 (s, 1H), 8.90-8.96 (m, 2H), 9.04 (d, J=0.75 Hz, 1H), 9.37 (d, J=1.25 Hz, 1H), 9.48 (d, J=2.51 Hz, 1H), 14.62 (brs, 1H); ESIMS found for C₃₁H₂₄N₈ m/z 509.2 (M+1).

5-(4-Methylpyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 109

White solid (23.8 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.76 (s, 3H), 7.53 (t, J=7.80 Hz, 1H), 7.86 (d, J=7.65 Hz, 1H), 8.01 (d, J=7.53 Hz, 1H), 8.12 (d, J=5.90 Hz, 1H), 8.83 (s, 1H), 8.91 (d, J=5.90 Hz, 1H), 9.03 (d, J=6.40 Hz, 2H), 9.16 (d, J=5.77 Hz, 2H), 9.26 (s, 1H), 9.41 (s, 1H), 13.94 (brs, 1H), 14.89 (brs, 1H); ESIMS found for C₂₄H₁₇N₇ m/z 404.1 (M+1).

2-Phenyl-N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide 114

White solid (16.5 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.77 (s, 2H), 7.26 (t, J=7.52 Hz, 1H), 7.35 (t, J=7.53 Hz, 2H), 7.39-7.46 (m, 3H), 7.66 (d, J=7.78 Hz, 1H), 7.72 (d, J=7.40 Hz, 1H), 8.42 (s, 1H), 8.44 (s, 1H), 8.77 (brs, 1H), 8.79 (s, 2H), 8.93 (s, 1H), 9.01 (s, 1H), 9.03 (d, J=1.63 Hz, 1H), 9.31 (s, 1H), 10.61 (s, 1H), 13.54 (s, 1H); ESIMS found for C₃₁H₂₂N₈O m/z 523.2 (M+1).

N-(5-(3-(4-(Pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide 121

White solid (24.0 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.97 (t, J=7.40 Hz, 3H), 1.69 (sxt, J=7.38 Hz, 2H), 2.52-2.55 (m, 2H), 7.51 (t, J=7.84 Hz, 1H), 7.83 (d, J=8.03 Hz, 1H), 8.00 (d, J=7.65 Hz, 1H), 9.04 (s, 1H), 9.12-9.17 (m, 2H), 9.18-9.23 (m, 3H), 9.31 (s, 1H), 9.36 (d, J=1.00 Hz, 1H), 9.41 (s, 1H), 11.38 (s, 1H), 13.86 (brs, 1H), 14.83 (s, 1H); ESIMS found for C₂₇H₂₂N₈O m/z 475.1 (M+1).

N-(5-(3-(4-(Pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide 126

White solid (54.7 mg, 0.11 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.19-1.39 (m, 3H), 1.44-1.57 (m, 2H), 1.64-1.72 (m, 1H), 1.75-1.84 (m, 2H), 1.91 (d, J=12.55 Hz, 2H), 2.39-2.47 (m, 1H), 7.43 (t, J=7.84 Hz, 1H), 7.66 (d, J=7.91 Hz, 1H), 7.72 (d, J=7.53 Hz, 1H), 8.40-8.45 (m, 2H), 8.73 (d, J=2.26 Hz, 1H), 8.78 (d, J=6.15 Hz, 2H), 8.96-9.03 (m, 3H), 9.32 (d, J=1.00 Hz, 1H), 10.25 (s, 1H), 13.53 (s, 1H), 14.41 (s, 1H); ESIMS found for C₃₀H₂₆N₈O m/z 515.2 (M+1).

N-((5-(3-(4-(Pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine 136

White solid (72.6 mg, 0.16 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.29 (t, J=6.96 Hz, 3H), 3.03-3.13 (m, 3H), 4.42 (t, J=4.64 Hz, 2H), 7.56 (t, J=7.65 Hz, 1H), 7.87-7.96 (m, 2H), 8.16 (d, J=7.03 Hz, 1H), 8.55-8.63 (m, 1H), 8.95 (brs, 1H), 9.03-9.12 (m, 2H), 9.14 (brs, 1H), 9.22 (s, 1H), 9.39 (s, 1H), 9.50-9.63 (m, 2H), 14.81 (brs, 1H); ESIMS found for C₂₆H₂₂N₈ m/z 447.1 (M+1).

N-(5-(3-(4-(Pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide 141

White solid (28.6 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.52 (t, J=7.78 Hz, 1H), 7.60-7.74 (m, 4H), 7.84 (d, J=8.16 Hz, 1H), 8.09-8.13 (m, 2H), 8.16 (d, J=7.40 Hz, 1H), 8.45-8.53 (m, 1H), 9.03 (d, J=4.89 Hz, 1H), 9.05-9.12 (m, 1H), 9.14 (s, 1H), 9.23 (s, 1H), 9.32 (s, 1H), 9.39 (d, J=1.13 Hz, 2H), 11.05 (s, 1H), 14.72 (brs, 1H); ESIMS found for C₃₀H₂₀N₈O m/z 509.2 (M+1).

N-(5-(3-(4-(Pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide 148

White solid (63.7 mg, 0.13 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.94 (t, J=7.40 Hz, 3H), 1.39 (sxt, J=7.80 Hz, 2H), 1.66 (quin, J=7.52 Hz, 2H), 2.43 (t, J=7.28 Hz, 2H), 7.40-7.49 (m, 2H), 7.69-7.79 (m, 1H), 8.07-8.14 (m, 1H), 8.15-8.23 (m, 1H), 8.73-8.86 (m, 3H), 8.90-9.00 (m, 2H), 9.05 (d, J=0.75 Hz, 1H), 9.34 (s, 1H), 10.34 (s, 1H), 14.43 (s, 1H); ESIMS found for C₂₈H₂₄N₈O m/z 489.1 (M+1).

1-Cyclopentyl-N-((5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine 154

White solid (40.3 mg, 0.08 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.21-1.34 (m, 2H), 1.47-1.65 (m, 4H), 1.76-1.87 (m, 2H), 2.21-2.30 (m, 1H), 3.00 (q, J=7.80 Hz, 3H), 4.43 (t, J=5.00 Hz, 2H), 7.55 (t, J=7.84 Hz, 1H), 7.89 (d, J=7.91 Hz, 2H), 8.16 (d, J=7.53 Hz, 1H), 8.54-8.61 (m, 1H), 8.97 (d, J=1.51 Hz, 1H), 9.04-9.12 (m, 2H), 9.16 (brs, 1H), 9.22 (s, 1H), 9.37-9.47 (m, 3H), 9.57 (d, J=1.63 Hz, 1H), 14.80 (brs, 1H); ESIMS found for C₃₀H₂₈N₈ m/z 501.2 (M+1).

N-(5-(3-(4-(Piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide 157

White solid (8.2 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.14 (t, J=7.47 Hz, 3H), 1.67-1.75 (m, 2H), 1.89-1.99 (m, 4H), 2.42 (q, J=7.40 Hz, 4H), 3.75-3.87 (m, 4H), 7.25 (d, J=2.76 Hz, 2H), 8.75 (d, J=2.13 Hz, 1H), 8.96 (s, 1H), 8.98 (brs, 1H), 9.01 (s, 1H), 9.33 (s, 1H), 10.35 (s, 1H), 14.42 (brs, 1H); ESIMS found for C₂₆H₂₆N₈O m/z 467.2 (M+1).

N-(5-(3-(4-(Piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide 175

White solid (10.6 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.84-0.91 (m, 4H), 1.62-1.70 (m, 2H), 1.78-1.89 (m, 5H), 3.58-3.65 (m, 4H), 6.55 (d, J=7.78 Hz, 1H), 7.02 (d, J=7.76 Hz, 1H), 7.09 (t, J=7.80 Hz, 1H), 8.70 (d, J=2.26 Hz, 1H), 8.92 (s, 1H), 8.95 (s, 1H), 8.96 (d, J=1.88 Hz, 1H), 9.28 (s, 1H), 10.60 (s, 1H), 13.06 (s, 1H), 14.24 (brs, 1H); ESIMS found for C₂₇H₂₆N₈O m/z 479.1 (M+1).

5-(5-((3,3-Difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine 181

White solid (12.7 mg, 0.02 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.97 (brs, 2H), 2.35 (brs, 4H), 2.72-2.85 (m, 2H), 3.91 (brs, 2H), 4.12 (brs, 6H), 7.54 (t, J=8.04 Hz, 1H), 7.70 (d, J=7.65 Hz, 1H), 7.85 (d, J=8.03 Hz, 1H), 9.16 (brs, 1H), 9.38 (s, 1H), 9.62 (brs, 1H), 9.79 (s, 1H), 9.96 (brs, 1H); ESIMS found for C₂₈H₂₈F₂N₈ m/z 515.2 (M+1).

3-Methyl-N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide 184

White solid (12.7 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ p 0.98 (d, J=6.65 Hz, 6H), 2.09-2.19 (m, 1H), 2.32 (d, J=6.90 Hz, 3H), 7.51 (t, J=7.80 Hz, 1H), 7.71 (d, J=7.65 Hz, 1H), 7.75 (d, J=7.78 Hz, 1H), 8.50 (d, J=1.51 Hz, 1H), 8.95 (d, J=1.51 Hz, 1H), 8.99 (s, 1H), 9.03 (brs, 1H), 9.16 (s, 1H), 9.36 (d, J=1.13 Hz, 1H), 10.08 (brs, 1H) 10.58 (brs, 1H), 13.92 (brs, 1H), 14.65 (s, 1H); ESIMS found for C₂₇H₂₅N₉O m/z 492.2 (M+1).

N-(5-(3-(4-(4-Methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide 190

White solid (6.6 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.29 (s, 9H), 2.29 (s, 3H), 7.36 (t, J=7.92 Hz, 1H), 7.50 (dd, J=7.92 Hz, J=1.76 Hz, 2H), 8.07 (s, 1H), 8.77 (s, 1H), 8.86 (s, 1H), 8.95 (s, 2H), 8.99 (d, J=1.38 Hz, 1H), 9.33 (s, 1H), 9.62 (s, 1H), 13.62 (brs, 1H), 14.44 (brs, 1H); ESIMS found for C₂₇H₂₅N₉O m/z 492.1 (M+1).

3-(4-(4-Methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 196

White solid (16.7 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.92 (brs, 2H), 2.06 (brs, 2H), 2.52 (d, J=0.88 Hz, 3H), 3.21 (brs, 2H), 3.48 (brs, 2H), 4.80 (d, J=2.51 Hz, 2H), 7.50 (t, J=7.97 Hz, 1H), 7.71 (d, J=7.40 Hz, 1H), 7.76 (d, J=8.16 Hz, 1H), 8.46 (s, 1H), 9.11 (s, 1H), 9.17 (s, 1H), 9.38 (d, J=1.25 Hz, 1H), 9.41 (brs, 1H), 9.62 (d, J=1.63 Hz, 1H), 10.24 (s, 1H), 11.70 (brs, 1H), 14.89 (brs, 1H); ESIMS found for C₂₇H₂₅N₉ m/z 476.2 (M+1).

N-(5-(3-(4-(4-Methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide 202

White solid (42.2 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.79-1.90 (m, 1H), 1.94-2.03 (m, 1H), 2.11-2.22 (m, 2H), 2.23-2.35 (m, 3H), 2.39 (s, 3H), 7.44 (t, J=7.88 Hz, 1H), 7.61 (dd, J=8.03, 4.14 Hz, 2H), 8.30 (s, 1H), 8.84 (d, J=2.38 Hz, 1H), 8.89 (t, J=2.01 Hz, 1H), 8.95 (s, 1H), 9.02 (d, J=1.88 Hz, 1H), 9.34 (d, J=1.25 Hz, 1H), 9.41 (d, J=3.64 Hz, 1H), 10.12 (s, 1H), 13.75 (s, 1H), 14.48 (s, 1H); ESIMS found for C₂₇H₂₃N₉O m/z 490.1 (M+1).

5-(3-(4-(4-Methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine 211

White solid (44.3 mg, 0.10 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.95 (d, J=4.64 Hz, 3H), 3.24-3.34 (m, 4H), 3.39-3.48 (m, 2H), 3.66-3.76 (m, 2H), 4.42-4.55 (m, 2H), 6.72 (d, J=6.90 Hz, 1H), 7.17-7.26 (m, 2H), 8.09 (d, J=2.26 Hz, 1H), 8.43 (t, J=1.88 Hz, 1H), 8.67 (s, 1H), 8.99 (s, 1H), 9.33 (d, J=1.13 Hz, 1H), 10.90 (brs, 1H), 14.66 (brs, 1H); ESIMS found for C₂₃H₂₃N₉ m/z 426.1 (M+1).

N-(5-(3-(4-(4-Methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide 217

White solid (21.8 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.19 (d, J=6.78 Hz, 6H), 2.79 (spt, J=7.16 Hz, 1H), 2.94 (d, J=4.39 Hz, 3H), 3.29 (t, J=12.28 Hz, 4H), 3.41-3.53 (m, 8H), 4.42-4.55 (m, 2H), 6.72 (d, J=5.65 Hz, 1H), 7.16-7.27 (m, 2H), 9.02 (s, 1H), 9.25 (brs, 2H), 9.28 (brs, 1H), 9.36 (d, J=1.00 Hz, 1H), 10.73-10.85 (m, 1H), 11.20 (brs, 1H), 14.67 (brs, 1H); ESIMS found for C₂₇H₂₉N₉O m/z 496.2 (M+1).

3-(4-(4-Methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 223

White solid (14.8 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.33-1.45 (m, 1H), 1.67-1.87 (m, 6H), 2.92 (d, J=4.40 Hz, 3H), 2.94-3.04 (m, 2H), 3.26-3.36 (m, 2H), 3.38-3.53 (m, 5H), 3.62-3.71 (m, 2H), 4.52 (d, J=5.02 Hz, 4H), 6.69-6.75 (m, 1H), 7.17-7.25 (m, 2H), 8.95 (s, 1H), 8.97 (brs, 1H), 9.01 (s, 1H), 9.35 (d, J=1.13 Hz, 1H), 9.48 (s, 1H), 10.81 (brs, 1H), 14.58 (brs, 1H); ESIMS found for C₂₉H₃₃N₉ m/z 508.2 (M+1).

N-(5-(3-(4-(4-Methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide 229

White solid (13.9 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.53-1.65 (m, 2H), 1.66-1.85 (m, 4H), 1.88-1.99 (m, 2H), 2.91-3.04 (m, 4H), 3.23-3.33 (m, 4H), 3.41-3.52 (m, 2H), 4.40-4.53 (m, 2H), 6.73 (d, J=6.65 Hz, 1H), 7.17-7.28 (m, 2H), 9.04 (s, 1H), 9.26 (s, 2H), 9.30 (s, 1H), 9.37 (s, 1H), 10.81 (brs, 1H) 11.30 (s, 1H), 14.71 (brs, 1H); ESIMS found for C₂₉H₃₁N₉O m/z 522.3 (M+1).

3-(4-(4-Methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine 234

White solid (63.9 mg, 0.16 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.90 (d, J=4.52 Hz, 3H), 3.29 (t, J=12.32 Hz, 2H), 3.43 (q, J=10.04 Hz, 2H), 3.61 (d, J=9.91 Hz, 2H), 4.40 (brs, 2H), 6.74-6.83 (m, 1H), 7.21-7.33 (m, 2H), 9.02-9.09 (m, 1H), 9.28 (s, 1H), 9.38 (s, 1H), 9.55 (brs, 2H), 10.75 (brs, 1H), 14.72 (brs, 1H); ESIMS found for C₂₂H₂₁N₉ m/z 412.1 (M+1).

3-(1H-Benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c] pyridine 238

White solid (29.1 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.54 (dd, J=6.09, 3.20 Hz, 2H), 7.86-7.92 (m, 2H), 8.15 (dd, J=8.16, 5.52 Hz, 1H), 8.94 (d, J=5.27 Hz, 1H), 9.37 (d, J=8.28 Hz, 1H), 9.47 (d, J=1.00 Hz, 1H), 9.65 (brs, 1H), 9.77 (d, J=1.63 Hz, 1H), 15.28 (brs, 1H); ESIMS found for C₁₈H₁₂N₆ m/z 313.0 (M+1).

N-(5-(3-(1H-Benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide 244

White solid (11.0 mg, 0.02 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 3.87 (s, 2H), 7.27-7.33 (m, 1H), 7.37 (t, J=7.53 Hz, 2H), 7.41-7.46 (m, 2H), 7.68 (dd, J=6.28 Hz, J=3.16 Hz, 2H), 7.95 (dd, J=6.24 Hz, J=3.12 Hz, 2H), 9.25 (d, J=1.25 Hz, 1H), 9.31 (d, J=2.13 Hz, 1H), 9.39 (d, J=1.38 Hz, 1H), 9.41-9.45 (m, 2H); ESIMS found for C₂₆H₁₉N₇O m/z 446.1 (M+1).

N-(5-(3-(1H-Benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide 250

White solid (11.9 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.07 (s, 9H), 2.31 (s, 2H), 7.33-7.39 (m, 2H), 7.71-7.77 (m, 2H), 8.96 (s, 1H), 8.99 (s, 1H), 9.02 (d, J=1.88 Hz, 1H), 9.17 (d, J=0.88 Hz, 1H), 9.37 (d, J=1.13 Hz, 1H), 10.50 (s, 1H), 14.66 (brs, 1H); ESIMS found for C₂₄H₂₃N₇O m/z 426.1 (M+1).

N-(5-(3-(1H-Benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide 256

White solid (49.9 mg, 0.11 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.18-1.38 (m, 3H), 1.40-1.53 (m, 2H), 1.64-1.72 (m, 1H), 1.75-1.83 (m, 2H), 1.84-1.93 (m, 2H), 2.36-2.46 (m, 1H), 7.27 (dd, J=5.90, 3.14 Hz, 2H), 7.63-7.75 (m, 2H), 8.78 (s, 1H), 8.84 (s, 1H), 8.89 (d, J=1.63 Hz, 1H), 9.01 (s, 1H), 9.31 (d, J=1.13 Hz, 1H), 10.25 (s, 1H), 14.38 (s, 1H); ESIMS found for C₂₅H₂₃N₇O m/z 438.1 (M+1).

5-(4-Methylpyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 265

White solid (26.8 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.80 (s, 3H), 7.33 (t, J=7.78 Hz, 1H), 7.52 (d, J=7.53 Hz, 1H), 7.64 (d, J=7.03 Hz, 1H), 7.68 (dd, J=5.08, 2.95 Hz, 1H), 8.09 (d, J=4.39 Hz, 1H), 8.14 (d, J=6.02 Hz, 1H), 8.67 (d, J=1.88 Hz, 1H), 8.85 (d, J=1.13 Hz, 1H), 8.88 (d, J=6.02 Hz, 1H), 9.20 (s, 1H), 9.40 (d, J=1.26 Hz, 1H), 14.68 (brs, 1H); ESIMS found for C₂₃H₁₆N₆S m/z 409.0 (M+1).

N-(5-(3-(4-(Thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide 268

White solid (36.7 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.31 (s, 9H), 7.32 (t, J=7.76 Hz, 1H), 7.50 (d, J=7.91 Hz, 1H), 7.65 (d, J=7.65 Hz, 1H), 7.72 (dd, J=4.96, 3.07 Hz, 1H), 8.17 (d, J=4.77 Hz, 1H), 8.75 (d, J=2.38 Hz, 1H), 8.91 (s, 2H), 9.00 (s, 1H), 9.04 (d, J=1.63 Hz, 1H), 9.32 (d, J=1.13 Hz, 1H), 9.64 (s, 1H), 13.37 (s, 1H); ESIMS found for C₂₇H₂₃N₇OS m/z 494.1 (M+1).

N-(5-(3-(4-(Thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide 269

White solid (46.2 mg, 0.10 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.21 (d, J=6.78 Hz, 6H), 2.75 (spt, J=6.8 Hz, 1H), 7.34 (t, J=7.78 Hz, 1H), 7.53 (d, J=7.91 Hz, 1H), 7.65 (d, J=7.40 Hz, 1H), 7.76 (dd, J=5.02, 3.01 Hz, 1H), 8.15 (d, J=4.77 Hz, 1H), 8.71 (d, J=2.38 Hz, 1H), 9.07 (s, 1H), 9.12 (d, J=2.13 Hz, 1H), 9.22 (d, J=1.51 Hz, 1H), 9.28 (s, 1H), 9.37 (d, J=0.88 Hz, 1H), 10.90 (s, 1H), 14.61 (brs, 1H); ESIMS found for C₂₆H₂₁N₇OS m/z 480.2 (M+1).

N-(5-(3-(4-(Thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide 271

White solid (17.2 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 7.30-7.38 (m, 1H), 7.53 (d, J=7.78 Hz, 1H), 7.59-7.74 (m, 5H), 8.12 (brs, 1H), 8.14 (brs, 2H), 8.74 (d, J=2.01 Hz, 1H), 9.12 (s, 1H), 9.28 (brs, 1H), 9.29 (brs, 1H), 9.39 (d, J=1.00 Hz, 1H), 9.43 (brs, 1H), 11.11 (brs, 1H), 14.60 (brs, 1H); ESIMS found for C₂₉H₁₉N₇OS m/z 514.0 (M+1).

N-(5-(3-(4-(Thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide 277

White solid (33.0 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.98 (t, J=7.40 Hz, 3H), 1.71 (sxt, J=7.40 Hz, 2H), 2.43-2.48 (m, 2H), 7.34 (t, J=7.78 Hz, 1H), 7.53 (d, J=7.65 Hz, 1H), 7.65 (d, J=7.53 Hz, 1H), 7.77 (dd, J=5.02, 3.01 Hz, 1H), 8.15 (d, J=5.40 Hz, 1H), 8.70 (d, J=2.13 Hz, 1H), 9.09 (s, 1H), 9.12 (s, 1H), 9.23 (s, 1H), 9.28 (brs, 1H), 9.38 (d, J=1.00 Hz, 1H), 11.01 (brs, 1H), 14.64 (brs, 1H); ESIMS found for C₂₆H₂₁N₇OS m/z 480.0 (M+1).

N-(5-(3-(4-(Thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide 280

White solid (43.0 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.81-1.93 (m, 1H), 1.96-2.08 (m, 1H), 2.14-2.25 (m, 2H), 2.27-2.40 (m, 2H), 3.28-3.38 (m, 1H), 7.33 (t, J=7.78 Hz, 1H), 7.50 (d, J=7.78 Hz, 1H), 7.65 (d, J=7.15 Hz, 1H), 7.74 (dd, J=5.02, 3.01 Hz, 1H), 8.14-8.20 (m, 1H), 8.74 (brs, 1H), 8.82 (s, 1H), 9.01 (s, 2H), 9.05 (d, J=1.76 Hz, 1H), 9.33 (s, 1H), 10.22 (s, 1H), 13.38 (brs, 1H); ESIMS found for C₂₇H₂₁N₇OS m/z 492.1 (M+1).

N-Benzyl-1-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine 283

White solid (31.0 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 4.32 (t, J=5.71 Hz, 2H), 4.47 (t, J=5.27 Hz, 2H), 7.34 (t, J=7.72 Hz, 1H), 7.41-7.48 (m, 3H), 7.52 (d, J=7.28 Hz, 1H), 7.59 (dd, J=7.84, 1.69 Hz, 2H), 7.65 (d, J=8.28 Hz, 1H), 7.72 (dd, J=5.02, 3.01 Hz, 1H), 8.12 (d, J=4.64 Hz, 1H), 8.80 (brs, 1H), 8.90 (d, J=1.76 Hz, 1H), 8.95 (s, 1H), 9.13 (d, J=1.13 Hz, 1H), 9.36 (d, J=1.26 Hz, 1H), 9.47 (d, J=2.01 Hz, 1H), 9.72-9.83 (m, 2H), 13.45 (brs, 1H), 14.58 (brs, 1H); ESIMS found for C₃₀H₂₃N₇S m/z 514.1 (M+1).

N-(5-(3-(4-(Furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 288

White solid (17.0 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.99 (d, J=6.65 Hz, 6H), 2.11-2.22 (m, 1H), 2.35 (d, J=7.28 Hz, 2H), 7.32 (t, J=7.8 Hz, 1H), 7.37 (s, 1H), 7.49 (d, J=7.91 Hz, 1H), 7.55 (d, J=7.40 Hz, 1H), 7.85 (s, 1H), 8.91 (s, 1H), 9.06 (s, 1H), 9.15 (s, 1H), 9.27 (br. s., 2H), 9.38 (s, 1H), 11.01 (s, 1H), 14.64 (brs, 1H); ESIMS found for C₂₇H₂₃N₇O₂ m/z 478.2 (M+1).

N-((5-(3-(4-(Furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine 292

White solid (92.4 mg, 0.21 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.29 (t, J=7.15 Hz, 3H), 3.03-3.14 (m, 2H), 4.38-4.45 (m, 2H), 7.29-7.38 (m, 2H), 7.50 (d, J=8.16 Hz, 1H), 7.55 (d, J=7.40 Hz, 1H), 7.87 (s, 1H), 8.95 (s, 1H), 8.97 (s, 1H), 9.06 (s, 1H), 9.13 (s, 1H), 9.37 (s, 1H), 9.41-9.49 (m, 2H), 9.50 (s, 1H), 14.65 (brs, 1H); ESIMS found for C₂₅H₂₁NO₇O m/z 436.1 (M+1).

N-(5-(3-(4-(Furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide 294

White solid (34.7 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.30 (s, 9H), 7.30 (t, J=7.64 Hz, 1H), 7.38 (s, 1H), 7.46 (d, J=7.91 Hz, 1H), 7.55 (d, J=7.28 Hz, 1H), 7.83 (s, 1H), 8.87-8.95 (m, 3H), 8.98 (s, 1H), 9.04 (d, J=1.38 Hz, 1H), 9.32 (s, 1H), 9.64 (s, 1H), 13.35 (brs, 1H), 14.38 (brs, 1H); ESIMS found for C₂₇H₂₃N₇O₂ m/z 478.1 (M+1).

N-(5-(3-(4-(Furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide 295

White solid (26.4 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.20 (d, J=6.90 Hz, 6H), 2.75 (spt, J=6.76 Hz, 2H), 7.32 (t, J=7.8 Hz, 1H), 7.37 (d, J=1.25 Hz, 1H), 7.50 (d, J=7.78 Hz, 1H), 7.55 (d, J=7.03 Hz, 1H), 7.85 (t, J=1.57 Hz, 1H), 8.91 (s, 1H), 9.08 (d, J=0.88 Hz, 1H), 9.18 (d, J=2.01 Hz, 1H), 9.29 (s, 1H), 9.32 (d, J=1.88 Hz, 1H), 9.38 (d, J=1.00 Hz, 1H), 11.03 (s, 1H), 14.67 (brs, 1H); ESIMS found for C₂₆H₂₁N₇O₂ m/z 464.2 (M+1).

5-(3-(4-(Furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c] pyridin-5-yl)-N-isopropylpyridin-3-amine 298

White solid (32.5 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.26 (d, J=6.27 Hz, 6H), 3.88-3.96 (m, 1H), 7.30-7.37 (m, 2H), 7.52 (d, J=7.91 Hz, 1H), 7.55 (d, J=7.53 Hz, 1H), 7.87 (s, 1H), 8.12 (d, J=2.13 Hz, 1H), 8.34 (s, 1H), 8.70 (s, 1H), 8.92 (s, 1H), 9.08 (s, 1H), 9.36 (d, J=0.88 Hz, 1H), 14.77 (brs, 1H); ESIMS found for C₂₅H₂₅N₇O m/z 436.2 (M+1).

N-(5-(3-(4-(Furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide 304

White solid (42.3 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.93 (t, J=7.34 Hz, 3H), 1.38 (sxt, J=7.64 Hz, 2H), 1.65 (quin, J=7.50 Hz, 2H), 2.42 (t, J=7.40 Hz, 2H), 7.30 (t, J=7.64 Hz, 1H), 7.39 (s, 1H), 7.47 (d, J=7.65 Hz, 1H), 7.55 (d, J=7.91 Hz, 1H), 7.83 (t, J=1.69 Hz, 1H), 8.83 (d, J=2.26 Hz, 1H), 8.88 (s, 1H), 8.97 (s, 2H), 9.05 (d, J=1.76 Hz, 1H), 9.33 (d, J=1.00 Hz, 1H), 10.36 (s, 1H), 13.34 (brs, 1H), 14.39 (s, 1H); ESIMS found for C₂₇H₂₃N₇O₂ m/z 478.1 (M+1).

N-(5-(3-(4-(Furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide 306

White solid (23.4 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.81-1.92 (m, 1H), 1.94-2.06 (m, 1H), 2.14-2.24 (m, 2H), 2.26-2.37 (m, 2H), 3.30 (quin, J=8.04 Hz, 1H), 7.31 (t, J=7.8 Hz, 1H), 7.38 (s, 1H), 7.48 (d, J=7.53 Hz, 1H), 7.55 (d, J=6.65 Hz, 1H), 7.84 (t, J=1.69 Hz, 1H), 8.89 (s, 2H), 8.99 (s, 1H), 9.04 (s, 1H), 9.09 (d, J=1.88 Hz, 1H), 9.34 (d, J=1.13 Hz, 1H), 10.28 (s, 1H), 13.36 (s, 1H), 14.41 (brs, 1H); ESIMS found for C₂₇H₂₁N₇O₂ m/z 476.1 (M+1).

1-Cyclopentyl-N-((5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine 310

White solid (62.4 mg, 0.13 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.21-1.33 (m, 2H), 1.45-1.55 (m, 2H), 1.56-1.65 (m, 2H), 1.76-1.86 (m, 2H), 2.26 (spt, J=7.88 Hz, 1H), 2.97-3.05 (m, 2H), 4.43 (d, J=5.02 Hz, 2H), 7.33 (t, J=7.76 Hz, 1H), 7.36 (d, J=1.51 Hz, 1H), 7.50 (d, J=7.53 Hz, 1H), 7.56 (d, J=7.40 Hz, 1H), 7.86 (t, J=1.69 Hz, 1H), 8.98 (s, 1H), 9.03 (d, J=1.38 Hz, 1H), 9.15 (d, J=1.00 Hz, 1H), 9.17 (brs, 1H), 9.38 (d, J=1.13 Hz, 1H), 9.40-9.48 (m, 2H), 9.54 (d, J=1.76 Hz, 1H), 14.70 (brs, 1H); ESIMS found for C₂₉H₂₇N₇O m/z 490.1 (M+1).

N-(5-(3-(4-(Thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide 313

White solid (22.8 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.17 (t, J=7.59 Hz, 3H), 2.46 (q, J=7.64 Hz, 6H), 7.28 (dd, J=8.16, 3.14 Hz, 1H), 7.31-7.35 (m, 1H), 7.48 (d, J=7.78 Hz, 1H), 7.64-7.70 (m, 2H), 8.19 (d, J=2.64 Hz, 1H), 8.74 (d, J=1.88 Hz, 1H), 9.00 (s, 1H), 9.06 (d, J=1.38 Hz, 1H), 9.15 (s, 1H), 9.33 (s, 1H), 10.35 (s, 1H), 13.43 (brs, 1H); ESIMS found for C₂₅H₁₉N₇OS m/z 466.0 (M+1).

N,N-Dimethyl-5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine 319

White solid (88.0 mg, 0.20 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 3.09 (s, 6H), 7.24 (dd, J=5.08, 3.70 Hz, 1H), 7.32 (t, J=7.78 Hz, 1H), 7.48 (d, J=7.78 Hz, 1H), 7.63 (dd, J=5.08, 0.82 Hz, 1H), 7.68 (d, J=7.53 Hz, 1H), 7.78 (t, J=2.40 Hz, 1H), 8.17 (d, J=2.89 Hz, 1H), 8.21 (d, J=2.89 Hz, 1H), 8.72 (d, J=1.51 Hz, 1H), 9.15 (s, 1H), 9.32 (d, J=1.13 Hz, 1H), 13.43 (s, 1H), 14.36 (s, 1H); ESIMS found for C₂₄H₁₉N₇S m/z 438.0 (M+1).

N-(5-(3-(4-(Thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide 320

White solid (33.1 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.32 (s, 9H), 7.26-7.37 (m, 2H), 7.50 (d, J=7.65 Hz, 1H), 7.67 (d, J=7.65 Hz, 1H), 7.72 (d, J=5.40 Hz, 1H), 8.22 (d, J=3.39 Hz, 1H), 9.15 (d, J=1.26 Hz, 1H), 9.18 (brs, 1H), 9.22 (s, 1H), 9.30 (brs, 1H), 9.36 (d, J=1.13 Hz, 1H), 10.17 (brs, 1H), 13.50 (s, 1H), 14.61 (brs, 1H); ESIMS found for C₂₇H₂₃N₇OS m/z 494.1 (M+1).

N-(5-(3-(4-(Thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide 321

White solid (42.5 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.21 (d, J=6.90 Hz, 6H), 2.77 (spt, J=6.68 HzHz, 1H), 7.28-7.36 (m, 2H), 7.47-7.52 (m, 1H), 7.63-7.69 (m, 1H), 7.76 (dd, J=5.02, 1.00 Hz, 1H), 8.17-8.23 (m, 1H), 9.16 (dd, J=5.46, 1.82 Hz, 2H), 9.22 (d, J=1.25 Hz, 1H), 9.37 (d, J=1.26 Hz, 2H), 11.05 (s, 1H), 14.64 (brs, 1H); ESIMS found for C₂₆H₂₁N₇OS m/z 480.2 (M+1).

N,N-Dimethyl-1-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine 325

White solid (10.1 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.82 (d, J=4.14 Hz, 6H), 4.57 (d, J=4.27 Hz, 2H), 7.26-7.32 (m, 1H), 7.34 (d, J=7.64 Hz, 1H), 7.51 (d, J=7.91 Hz, 1H), 7.67 (d, J=7.78 Hz, 1H), 7.74 (d, J=5.02 Hz, 1H), 8.21 (d, J=3.01 Hz, 1H), 9.01 (s, 1H), 9.03 (brs, 1H), 9.27 (s, 1H), 9.38 (d, J=1.13 Hz, 1H), 9.49 (s, 1H), 10.93 (brs, 1H), 14.65 (brs, 1H); ESIMS found for C₂₅H₂₅N₇S m/z 452.1 (M+1).

N-(5-(3-(4-(Thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide 329

White solid (26.7 mg, 0.06 mmol). ¹H NMR (CD₃OD, 400 MHz) δ ppm 1.08 (t, J=7.40 Hz, 3H), 1.81 (sxt, J=7.38 Hz, 2H), 2.50 (t, J=7.40 Hz, 2H), 7.27 (dd, J=5.02, 3.51 Hz, 1H), 7.43 (t, J=8.00 Hz, 1H), 7.53-7.66 (m, 3H), 7.86 (d, J=2.64 Hz, 1H), 9.01 (d, J=13.80 Hz, 2H), 9.07-9.14 (m, 2H), 9.19 (s, 1H); ESIMS found for C₂₆H₂₁N₇OS m/z 480.1 (M+1).

N-(5-(3-(4-(Thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide 331

White solid (49.5 mg, 0.10 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.86-0.94 (m, 4H), 1.89 (quin, J=6.15 Hz, 1H), 7.27 (dd, J=5.02, 3.76 Hz, 1H), 7.32 (t, J=7.80 Hz, 1H), 7.48 (d, J=7.65 Hz, 1H), 7.63 (dd, J=5.02, 0.88 Hz, 1H), 7.67 (d, J=7.40 Hz, 1H), 8.19 (dd, J=3.64 Hz, J=0.88 Hz, 1H), 8.74 (d, J=2.26 Hz, 1H), 8.97 (t, J=2.01 Hz, 1H), 9.06 (d, J=1.76 Hz, 1H), 9.16 (s, 1H), 9.32 (d, J=1.13 Hz, 1H), 10.63 (s, 1H), 13.42 (s, 1H), 14.38 (s, 1H); ESIMS found for C₂₆H₁₉N₇OS m/z 478.1 (M+1).

5-(5-((3,3-Difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 337

White solid (59.7 mg, 0.12 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.21-2.31 (m, 2H), 2.76-2.83 (m, 2H), 2.93-3.03 (m, 2H), 3.84 (brs, 2H), 7.28 (dd, J=5.02, 3.76 Hz, 1H), 7.32 (t, J=7.72 Hz, 1H), 7.49 (d, J=8.03 Hz, 1H), 7.65-7.71 (m, 2H), 8.21 (d, J=2.64 Hz, 1H), 8.46 (s, 1H), 8.60 (d, J=1.63 Hz, 1H), 9.23 (s, 1H), 9.32 (d, J=2.13 Hz, 1H), 9.34 (d, J=1.13 Hz, 1H), 13.44 (s, 1H), 14.37 (s, 1H); ESIMS found for C₂₇H₂₁F₂N₇S m/z 514.1 (M+1).

N-(5-(3-(4-(5-Fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 340

White solid (35.0 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.98 (d, J=6.65 Hz, 6H), 2.14 (non, J=7.16 Hz, 1H), 2.31 (d, J=7.03 Hz, 2H), 6.87 (dd, J=4.20, 2.20 Hz, 1H), 7.32 (t, J=7.76 Hz, 1H), 7.48 (d, J=7.91 Hz, 1H), 7.66 (d, J=7.28 Hz, 1H), 7.79 (t, J=3.95 Hz, 1H), 8.96 (d, J=1.63 Hz, 1H), 9.08-9.13 (m, 2H), 9.15 (d, J=0.88 Hz, 1H), 9.36 (d, J=1.13 Hz, 1H), 10.62 (s, 1H), 13.50 (brs, 1H), 14.52 (brs, 1H); ESIMS found for C₂₇H₂₂FN₇OS m/z 512.3 (M+1).

3-(4-(5-Fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 352

White solid (66.3 mg, 0.13 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.85-1.96 (m, 2H), 2.01-2.11 (m, 2H), 3.11-3.24 (m, 2H), 3.43-3.54 (m, 2H), 4.67 (brs, 2H), 6.91 (brs, 1H), 7.32 (t, J=7.78 Hz, 1H), 7.51 (d, J=7.91 Hz, 1H), 7.65 (d, J=5.40 Hz, 1H), 7.82 (d, J=3.51 Hz, 1H), 9.11-9.33 (m, 2H), 9.24 (s, 1H), 9.40 (s, 1H), 9.53 (brs, 1H), 11.49 (brs, 1H), 14.79 (brs, 1H); ESIMS found for C₂₇H₂₂FN₇S m/z 496.1 (M+1).

N-(5-(3-(4-(5-Fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide 358

White solid (69.1 mg, 0.14 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.79-1.93 (m, 1H), 1.95-2.07 (m, 1H), 2.14-2.26 (m, 2H), 2.27-2.38 (m, 2H), 3.30-3.42 (m, 1H), 6.89 (dd, J=4.14, 2.13 Hz, 1H), 7.27-7.37 (m, 1H), 7.48 (d, J=7.91 Hz, 1H), 7.58-7.68 (m, 1H), 7.83 (t, J=3.76 Hz, 1H), 9.06-9.20 (m, 1H), 9.16 (brs, 2H), 9.26-9.34 (m, 1H), 9.36 (d, J=5.90 Hz, 1H), 10.75-10.96 (m, 1H), 13.54 (brs, 1H), 14.63 (brs, 1H); ESIMS found for C₂₇H₂₀FN₇OS m/z 510.1 (M+1).

3-Methyl-N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide 366

White solid (19.2 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.98 (d, J=6.65 Hz, 5H), 2.10-2.23 (m, 1H), 2.34 (d, J=7.15 Hz, 2H), 2.57 (s, 3H), 6.99 (d, J=2.51 Hz, 1H), 7.29 (t, J=7.92 Hz, 1H), 7.45 (d, J=7.78 Hz, 1H), 7.57 (d, J=7.15 Hz, 1H), 8.01 (d, J=3.64 Hz, 1H), 9.11 (d, J=1.88 Hz, 1H), 9.16 (d, J=1.63 Hz, 1H), 9.20 (s, 1H), 9.22 (d, J=1.00 Hz, 1H), 9.36 (d, J=1.13 Hz, 1H), 10.97 (s, 1H), 14.62 (brs, 1H); ESIMS found for C₂₈H₂₅N₇OS m/z 508.2 (M+1).

5-(3-(4-(5-Methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine 367

White solid (35.9 mg, 0.08 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.57 (s, 3H), 6.99 (dd, J=3.51, 1.13 Hz, 1H), 7.29 (t. J=7.76 Hz, 1H), 7.45 (d, J=8.03 Hz, 1H), 7.58 (d, J=7.03 Hz, 1H), 7.99 (d, J=3.64 Hz, 1H), 8.11 (d, J=2.26 Hz, 1H), 8.38 (t, J=1.88 Hz, 1H), 8.61 (s, 1H), 9.14 (d, J=1.13 Hz, 1H), 9.35 (d, J=1.25 Hz, 1H), 13.47 (brs, 1H) 14.61 (brs, 1H); ESIMS found for C₂₃H₁₇N₇S m/z 424.0 (M+1).

N-(5-(3-(4-(5-Methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide 373

White solid (21.3 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.17 (d, J=6.90 Hz, 6H), 2.61 (s, 3H), 2.68 (sxt, J=6.80 Hz, 2H), 6.96 (dd, J=3.58, 1.07 Hz, 1H), 7.29 (t, J=7.76 Hz, 1H), 7.44 (d, J=7.65 Hz, 1H), 7.61 (d, J=7.40 Hz, 1H), 7.94 (d, J=3.51 Hz, 1H), 8.85 (d, J=2.38 Hz, 1H), 8.97 (t, J=2.20 Hz, 1H), 9.10 (d, J=2.01 Hz, 1H), 9.28 (s, 1H), 9.32 (d, J=1.25 Hz, 1H), 10.28 (s, 1H), 13.40 (brs, 1H) 14.36 (s, 1H); ESIMS found for C₂₇H₂₃N₇OS m/z 494.1 (M+1).

3-(4-(5-Methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 379

White solid (26.6 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.31-1.45 (m, 1H), 1.66-1.77 (m, 2H), 1.80 (brs, 3H), 2.64 (s, 3H), 2.90-3.02 (m, 2H), 3.42 (d, J=10.92 Hz, 2H), 4.53 (d, J=4.52 Hz, 2H), 6.97 (dd, J=3.522 Hz, J=0.88 Hz, 1H), 7.30 (t, J=7.80 Hz, 1H), 7.46 (d, J=7.78 Hz, 1H), 7.60 (d, J=7.28 Hz, 1H), 7.96 (d, J=3.51 Hz, 1H), 9.02 (d, J=9.66 Hz, 2H), 9.35 (s, 1H), 9.37 (d, J=1.00 Hz, 1H), 9.52 (d, J=1.76 Hz, 1H), 10.74 (brs, 1H), 13.48 (brs, 1H), 14.62 (brs, 1H); ESIMS found for C₂₉H₂₇N₇S m/z 506.1 (M+1).

N-(5-(3-(4-(5-Methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide 381

White solid (52.9 mg, 0.11 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.96 (t, J=7.34 Hz, 3H), 1.67 (sxt, J=7.35 Hz, 2H), 2.39 (t, J=7.28 Hz, 2H), 2.61 (s, 3H), 6.95 (d, J=2.64 Hz, 1H), 7.28 (t, J=7.8 Hz, 1H), 7.43 (d, J=7.78 Hz, 1H), 7.61 (d, J=7.53 Hz, 1H), 7.91 (d, J=3.51 Hz, 1H), 8.81 (d, J=2.26 Hz, 1H), 8.96 (s, 1H), 9.10 (d, J=1.51 Hz, 1H), 9.29 (s, 1H), 9.32 (s, 1H), 10.30 (s, 1H), 13.39 (brs, 1H); ESIMS found for C₂₇H₂₃N₇OS m/z 494.1 (M+1).

N-(5-(3-(4-(5-Methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide 385

White solid (25.6 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.56-1.65 (m, 2H), 1.65-1.85 (m, 4H), 1.87-1.98 (m, 2H), 2.58 (s, 3H), 2.85-2.94 (m, 1H), 6.97 (dd, J=3.52 Hz, J=1.00 Hz, 1H), 7.29 (t, J=7.92 Hz, 1H), 7.44 (d, J=7.91 Hz, 1H), 7.59 (d, J=7.40 Hz, 1H), 7.98 (d, J=3.51 Hz, 1H), 8.98 (d, J=2.01 Hz, 1H), 9.08 (s, 1H), 9.13 (d, J=1.88 Hz, 1H), 9.25 (s, 1H), 9.34 (d, J=1.13 Hz, 1H), 10.61 (s, 1H), 13.44 (brs, 1H) 14.47 (s, 1H); ESIMS found for C₂₉H₂₅N₇OS m/z 520.1 (M+1).

N-(5-(3-(4-(5-Acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 392

White solid (9.1 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.98 (d, J=6.65 Hz, 6H), 2.14 (non, J=6.16 Hz, 1H), 2.33 (d, J=7.15 Hz, 2H), 2.60 (s, 3H), 7.36 (t, J=7.64 Hz, 1H), 7.59 (d, J=7.91 Hz, 1H), 7.81 (d, J=7.40 Hz, 1H), 8.07 (d, J=4.02 Hz, 1H), 8.29 (d, J=3.89 Hz, 1H), 9.05 (s, 1H), 9.08-9.14 (m, 2H), 9.17 (brs, 1H), 9.36 (s, 1H), 10.66 (brs, 1H), 13.58 (brs, 1H), 14.58 (brs, 1H); ESIMS found for C₂₉H₂₅N₇O₂S m/z 536.1 (M+1).

1-(5-(2-(5-(Pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one 394

White solid (72.3 mg, 0.17 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.66 (s, 3H), 7.38 (t, J=7.76 Hz, 1H), 7.59 (d, J=7.78 Hz, 1H), 7.90 (d, J=7.65 Hz, 1H), 7.92-7.98 (m, 1H), 8.06 (d, J=3.89 Hz, 1H), 8.12 (d, J=3.89 Hz, 1H), 8.77 (d, J=4.27 Hz, 1H), 8.87 (d, J=7.78 Hz, 1H), 9.28 (s, 1H), 9.37 (s, 1H), 9.54 (s, 1H), 13.60 (brs, 1H), 14.51 (s, 1H); ESIMS found for C₂₄H₁₆N₆OS m/z 437.0 (M+1).

N-(5-(3-(4-(5-Acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide 400

White solid (15.3 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.59 (s, 3H), 3.82 (s, 2H), 7.24-7.30 (m, 1H), 7.32-7.42 (m, 5H), 7.58 (d, J=7.78 Hz, 1H), 7.83 (d, J=7.65 Hz, 1H), 8.08 (d, J=4.02 Hz, 1H), 8.26 (d, J=4.02 Hz, 1H), 9.17 (s, 1H), 9.20 (d, J=1.13 Hz, 1H), 9.22 (s, 2H), 9.37 (d, J=1.13 Hz, 1H), 11.25 (s, 1H), 13.62 (brs, 1H), 14.64 (brs, 1H); ESIMS found for C₃₂H₂₃N₇O₂S m/z 570.2 (M+1).

N-(5-(3-(4-(5-Acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide 406

White solid (6.5 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.06 (s, 9H), 2.31 (s, 2H), 2.60 (s, 3H), 7.38 (t, J=7.84 Hz, 1H), 7.60 (d, J=7.65 Hz, 1H), 7.83 (d, J=7.40 Hz, 1H), 8.05 (d, J=3.89 Hz, 1H), 8.29 (d, J=3.39 Hz, 1H), 8.99 (brs, 1H), 9.03 (t, J=4.71 Hz, 1H), 9.10-9.17 (m, 2H), 9.38 (s, 1H), 10.44 (brs, 1H), 13.60 (brs, 1H), 14.54 (s, 1H); ESIMS found for C₃₀H₂₇N₇O₂S m/z 550.2 (M+1).

N-(5-(3-(4-(5-Acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide 412

White solid (51.9 mg, 0.09 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.20-1.37 (m, 3H), 1.39-1.52 (m, 2H), 1.67 (d, J=11.29 Hz, 1H), 1.74-1.82 (m, 2H), 1.87 (d, J=13.30 Hz, 2H), 2.37-2.46 (m, 1H), 2.58 (s, 3H), 7.37 (t, J=7.78 Hz, 1H), 7.59 (d, J=8.03 Hz, 1H), 7.81 (d, J=7.65 Hz, 1H), 8.03 (d, J=4.14 Hz, 1H), 8.31 (d, J=3.89 Hz, 1H), 8.84-8.89 (m, 2H), 9.02 (s, 1H), 9.05 (d, J=1.63 Hz, 1H), 9.34 (d, J=0.88 Hz, 1H), 10.19 (s, 1H), 13.55 (s, 1H), 14.46 (s, 1H); ESIMS found for C₃₁H₂₇N₇O₂S m/z 562.2 (M+1).

N-(3-Fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c] pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide 421

White solid (42.4 mg, 0.08 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.66 (s, 3H), 2.88 (s, 3H), 4.24 (d, J=6.15 Hz, 2H), 7.16-7.22 (m, 1H), 7.40 (t, J=7.80 Hz, 1H), 7.60 (dd, J=12.30, 7.91 Hz, 2H), 7.68 (t, J=6.40 Hz, 1H), 7.96-8.03 (m, 2H), 8.19-8.31 (m, 1H), 8.71 (d, J=1.13 Hz, 1H), 8.82 (d, J=5.52 Hz, 1H), 9.07 (s, 1H), 9.38 (d, J=1.25 Hz, 1H), 14.57 (brs, 1H); ESIMS found for C₂₇H₂₂FN₇O₂S m/z 528.1 (M+1).

N-(5-(3-(4-(3-Fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide 424

White solid (35.0 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.31 (s, 9H), 2.89 (s, 3H), 4.31 (d, J=5.77 Hz, 2H), 7.22 (d, J=9.29 Hz, 1H), 7.43 (t, J=7.76 Hz, 1H), 7.63 (d, J=7.53 Hz, 1H), 7.67 (d, J=7.91 Hz, 1H), 7.73 (t, J=6.02 Hz, 1H), 8.00 (s, 1H), 8.34 (d, J=11.29 Hz, 1H), 9.08 (s, 1H), 9.23 (s, 1H), 9.34 (s, 2H), 9.38 (d, J=1.00 Hz, 1H), 10.28 (s, 1H), 14.73 (brs, 1H); ESIMS found for C₃₁H₂₉FN₈O₃S m/z 613.2 (M+1).

N-(5-(3-(4-(3-Fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide 427

White solid (12.8 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.90 (s, 3H), 4.30 (d, J=6.02 Hz, 2H), 7.09 (d, J=8.41 Hz, 1H), 7.42 (t, J=7.78 Hz, 1H), 7.59-7.66 (m, 4H), 7.66-7.76 (m, 2H), 7.99 (brs, 1H), 8.08 (d, J=1.26 Hz, 1H), 8.10 (s, 1H), 8.34-8.45 (m, 1H), 9.06 (d, J=0.88 Hz, 1H), 9.20 (s, 1H), 9.25 (brs, 1H), 9.27 (s, 1H), 9.38 (d, J=1.13 Hz, 1H), 10.96 (s, 1H), 14.58 (brs, 1H); ESIMS found for C₃₃H₂₅FN₈O₃S m/z 633.2 (M+1).

N-(5-(3-(4-(3-Fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide 433

White solid (39.5 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1H NMR (400 MHz, DMSO-d₆) δ 0.97 (t, J=7.34 Hz, 3H), 1.68 (sxt, J=7.33 Hz, 2H), 2.43 (t, J=7.28 Hz, 2H), 2.89 (s, 3H), 4.32 (d, J=6.15 Hz, 2H), 7.20-7.27 (m, 1H), 7.42 (t, J=7.78 Hz, 1H), 7.63 (dd, J=11.48, 7.72 Hz, 2H), 7.72 (t, J=5.96 Hz, 1H), 8.01 (brs, 1H), 8.32 (d, J=2.26 Hz, 1H), 9.03 (d, J=1.13 Hz, 1H), 9.10 (s, 2H), 9.15 (s, 1H), 9.37 (d, J=1.25 Hz, 1H), 10.78 (brs, 1H), 14.60 (brs, 1H); ESIMS found for C₃₀H₂₇FN₈O₃S m/z 599.1 (M+1).

N-(5-(3-(4-(3-Fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl) cyclobutanecarboxamide 435

White solid (48.2 mg, 0.08 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.78-1.91 (m, 1H), 1.93-2.06 (m, 1H), 2.12-2.22 (m, 2H), 2.23-2.35 (m, 2H), 2.90 (s, 3H), 3.32 (quin, J=8.16 Hz, 1H), 4.33 (d, J=6.27 Hz, 2H), 7.23 (d, J=8.66 Hz, 1H), 7.40 (t, J=7.68 Hz, 1H), 7.62 (d, J=7.65 Hz, 2H), 7.72 (t, J=6.53 Hz, 1H), 8.00 (brs, 1H), 8.44 (brs, 1H), 8.89 (s, 1H), 8.91 (s, 1H), 8.98 (s, 1H), 9.00 (d, J=1.38 Hz, 1H), 9.33 (d, J=1.13 Hz, 1H), 10.17 (s, 1H), 13.47 (brs, 1H), 14.41 (s, 1H); ESIMS found for C₃₁H₂₇FN₈O₃S m/z 611.2 (M+1).

N-(3-(2-(5-(5-((Benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide 439

White solid (27.5 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.93 (s, 3H), 4.26-4.32 (m, 2H), 4.35 (d, J=6.40 Hz, 2H), 4.39-4.45 (m, 2H), 7.24-7.30 (m, 1H), 7.38-7.49 (m, 4H), 7.55 (d, J=6.40 Hz, 2H), 7.63 (d, J=7.40 Hz, 2H), 7.77 (t, J=6.59 Hz, 1H), 7.95 (d, J=1.51 Hz, 1H), 8.80 (s, 2H), 9.05 (s, 1H), 9.35 (d, J=1.13 Hz, 1H), 9.39 (s, 1H), 9.49-9.59 (m, 2H), 14.52 (s, 1H); ESIMS found for C₃₄H₂₉FN₈O₂S m/z 633.2 (M+1).

N-(3-Fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methane sulfonamide 442

White solid (56.8 mg, 0.11 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 2.94 (s, 3H), 4.35 (d, J=5.90 Hz, 2H), 7.27 (d, J=9.41 Hz, 1H), 7.42 (t, J=7.68 Hz, 1H), 7.63 (t, J=6.52 Hz, 2H), 7.75 (t, J=6.34 Hz, 1H), 7.97 (s, 1H), 8.36-8.44 (m, 1H), 9.06 (d, J=1.00 Hz, 1H), 9.27 (s, 1H), 9.37 (d, J=1.13 Hz, 1H), 9.50 (s, 2H), 14.56 (s, 1H); ESIMS found for C₂₅H₁₉FN₈O₂S m/z 515.1 (M+1).

N¹-(3-(2-(5-(5-((Ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine 448

White solid (31.6 mg, 0.06 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.28 (t, J=5.04 Hz, 3H), 2.76-2.88 (m, 6H), 3.00-3.11 (m, 2H), 3.29 (brs, 2H), 3.57 (brs, 2H), 4.39 (brs, 4H), 6.60 (d, J=11.44 Hz, 1H), 7.22 (brs, 1H), 7.31-7.48 (m, 2H), 7.53 (d, J=6.15 Hz, 1H), 7.70 (d, J=7.03 Hz, 1H), 9.02 (brs, 1H), 9.08 (brs, 1H), 9.19 (brs, 1H), 9.38 (s, 1H), 9.53 (brs, 1H), 9.62 (brs, 2H), 10.61 (brs, 1H), 14.88 (brs, 1H); ESIMS found for C₃₁H₃₂FN₉ m/z 550.2 (M+1).

N-(5-(3-(4-(3-((2-(Dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide 450

White solid (30.1 mg, 0.05 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.28 (s, 9H), 2.16-2.37 (m, 6H), 3.21-3.52 (m, 4H), 5.94 (brs, 1H), 6.46 (d, J=11.17 Hz, 1H), 7.31-7.40 (m, 2H), 7.47-7.52 (m, 2H), 7.55 (d, J=8.03 Hz, 1H), 8.81 (s, 1H), 8.94-9.01 (m, 3H), 9.31 (s, 1H), 9.58 (s, 1H), 13.37 (brs, 1H); ESIMS found for C₃₃H₃₄FN₉O m/z 592.3 (M+1).

N¹-(3-Fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine 454

White solid (39.4 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.19 (d, J=6.27 Hz, 6H), 2.68-2.86 (m, 6H), 3.19-3.28 (m, 2H), 3.45-3.54 (m, 2H), 3.63-3.73 (m, 1H), 5.86 (d, J=7.91 Hz, 1H), 6.54 (d, J=11.80 Hz, 1H), 7.35 (t, J=7.65 Hz, 1H), 7.39-7.45 (m, 2H), 7.49 (d, J=7.65 Hz, 1H), 7.55 (d, J=8.66 Hz, 1H), 7.66 (s, 1H), 8.01 (d, J=2.76 Hz, 1H), 8.49 (d, J=1.63 Hz, 1H), 8.91 (s, 1H), 9.29 (d, J=1.13 Hz, 1H), 13.38 (s, 1H), 14.33 (brs, 1H); ESIMS found for C₃₁H₃₂FN₉ m/z 550.2 (M+1).

N-(5-(3-(4-(3-((2-(Dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide 460

White solid (50.0 mg, 0.08 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 0.92 (t, J=7.28 Hz, 3H), 1.37 (sxt, J=7.76 Hz, 2H), 1.62 (quin, J=7.16 Hz, 2H), 2.39 (t, J=7.34 Hz, 2H), 2.59-2.83 (m, 5H), 3.43-3.53 (m, 2H), 6.17-6.32 (m, 1H), 6.53 (d, J=11.67 Hz, 1H), 7.36 (t, J=7.65 Hz, 1H), 7.41-7.53 (m, 3H), 7.56 (d, J=7.91 Hz, 1H), 8.82-8.88 (m, 2H), 8.97 (d, J=1.63 Hz, 1H), 9.00 (s, 1H), 9.32 (s, 1H), 10.24 (s, 1H), 13.39 (s, 1H), 14.37 (brs, 1H); ESIMS found for C₃₃H₃₄FN₉O m/z 592.2 (M+1).

N¹-(3-(2-(5-(5-(((Cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine 466

White solid (76.2 mg, 0.13 mmol). ¹H NMR (DMSO-d₆, 400 MHz) δ ppm 1.21-1.32 (m, 2H), 1.46-1.65 (m, 5H), 1.74-1.85 (m, 2H), 2.25 (spt, J=7.68 Hz, 2H), 2.82 (brs, 3H), 2.83 (brs, 3H), 2.94-3.02 (m, 2H), 3.26-3.33 (m, 2H), 3.58 (t, J=6.46 Hz, 2H), 4.40 (brs, 2H), 6.60 (d, J=11.54 Hz, 1H), 7.26 (d, J=1.76 Hz, 1H), 7.38-7.48 (m, 2H), 7.53 (d, J=7.28 Hz, 1H), 7.66 (d, J=7.65 Hz, 1H), 8.99 (brs, 1H), 9.03 (d, J=2.13 Hz, 1H), 9.15 (s, 1H), 9.38 (s, 1H), 9.39-9.47 (m, 2H), 9.49 (s, 1H), 10.52 (brs, 1H), 14.76 (brs, 1H); ESIMS found for C₃₅H₃₈FN₉ m/z 604.3 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine 937

White solid (40.0 mg, 0.10 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.69 (qd, J=12.40, 3.98 Hz, 2H), 2.01 (br d, J=12.35 Hz, 2H), 2.65-2.75 (m, 2H), 2.86-2.96 (m, 1H), 3.12 (br d, J=11.80 Hz, 2H), 7.26 (td, J=8.37, 2.20 Hz, 1H), 7.34 (t, J=7.82 Hz, 1H), 7.58 (dd, J=13.86, 7.55 Hz, 3H), 8.10 (br d, J=7.96 Hz, 1H), 8.29 (s, 1H), 8.44 (br d, J=10.70 Hz, 1H), 9.09 (d, J=1.10 Hz, 1H); ESIMS found for C₂₄H₂₁FN₆ m/z 413.2 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine 938

Off-white solid (190.0 mg, 0.44 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.61 (br s, 2H), 3.06 (t, J=5.63 Hz, 2H), 3.52 (br d, J=2.74 Hz, 2H), 6.93 (br s, 1H), 7.26 (td, J=8.51, 2.20 Hz, 1H), 7.36 (t, J=7.68 Hz, 1H), 7.53-7.59 (m, 2H), 7.61 (d, J=7.41 Hz, 1H), 8.07 (br d, J=7.14 Hz, 1H), 8.50 (br s, 1H), 8.52 (s, 1H), 9.12 (d, J=1.37 Hz, 1H); ESIMS found for C₂₄H₁₉FN₆ m/z 411.1 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine 939

Yellow solid (4.5 mg, 0.01 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.30 (td, J=8.23, 2.47 Hz, 1H), 7.37 (t, J=7.68 Hz, 1H), 7.55-7.65 (m, 3H), 8.01-8.12 (m, 2H), 8.22 (br s, 1H), 8.58-8.66 (m, 2H), 9.15 (d, J=1.10 Hz, 1H), 13.11 (br s, 1H), 13.37 (br s, 1H), 14.16 (br s, 1H); ESIMS found for C₂₂H₁₄FN₇ m/z 396.1 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine 940

Yellow solid (2.3 mg, 0.006 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 3.94 (s, 3H), 7.31 (br t, J=8.51 Hz, 1H), 7.37 (t, J=7.82 Hz, 1H), 7.55-7.67 (m, 3H), 7.95 (s, 1H), 8.07 (d, J=7.96 Hz, 1H), 8.19 (s, 1H), 8.57-8.64 (m, 2H), 9.14 (d, J=1.10 Hz, 1H), 13.35 (br s, 1H), 14.15 (br s, 1H); ESIMS found for C₂₃H₁₆FN₇ m/z 410.1 (M+1).

5-(1,2-Dimethyl-1H-imidazol-5-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 941

Brown solid (8.7 mg, 0.02 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.42 (s, 3H), 3.85 (s, 3H), 7.22-7.31 (m, 2H), 7.37 (t, J=7.82 Hz, 2H), 7.53-7.63 (m, 3H), 8.09 (d, J=7.96 Hz, 1H), 8.40 (br d, J=11.25 Hz, 1H), 8.64 (s, 1H), 9.24 (d, J=1.10 Hz, 1H), 13.38 (s, 1H), 14.27 (s, 1H); ESIMS found for C₂₄H₁₈FN₇ m/z 424.2 (M+1).

1-(6-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine 942

Brown solid (5.9 mg, 0.012 mmol, 12.02% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 3.66-3.75 (m, 2H), 3.94 (dt, J=12.97, 6.55 Hz, 1H), 4.23 (br t, J=7.41 Hz, 2H), 7.32 (td, J=8.51, 2.20 Hz, 1H), 7.37 (t, J=7.82 Hz, 1H), 7.49 (br d, J=7.14 Hz, 1H), 7.55-7.65 (m, 2H), 7.88 (s, 1H), 8.00 (br d, J=10.98 Hz, 1H), 8.08 (br d, J=6.59 Hz, 1H), 8.83 (s, 1H), 9.19 (s, 1H), 9.25 (d, J=0.82 Hz, 1H), 13.36 (br s, 2H); ESIMS found for C₂₆H₂₀FN₉ m/z 478.2 (M+H).

5-(5-(Cyclohexyloxy)pyridin-3-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 943

Off-white solid (57 mg, 0.102 mmol, 17.22% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.28-1.37 (m, 1H), 1.42 (dtd, J=13.21, 9.86, 9.86, 3.29 Hz, 2H), 1.50-1.62 (m, 3H), 1.70-181 (m, 2H), 1.94-2.05 (m, 2H), 4.54-4.63 (m, 1H), 7.25 (td, J=8.44, 2.33 Hz, 1H), 7.38 (t, J=7.82 Hz, 1H), 7.60 (dd, J=15.37, 7.68 Hz, 3H), 7.96-8.02 (m, 1H), 8.18 (d, J=7.68 Hz, 1H), 8.36 (d, J=2.74 Hz, 1H), 8.42 (br d, J=11.25 Hz, 1H), 8.91 (d, J=1.65 Hz, 1H), 8.99 (s, 1H), 9.31 (d, J=1.10 Hz, 1H), 13.44 (br s, 1H), 14.35 (br s, 1H); ESIMS found for C₃₀H₂₅FN₆O m/z 505.2 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy) pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 944

White solid (66 mg, 0.131 mmol, 49.2% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.85-1.97 (m, 2H), 2.11-2.21 (m, 2H), 3.10 (ddd, J=12.49, 8.51, 3.70 Hz, 2H), 3.25-3.32 (m, 2H), 4.89 (dt, J=7.27, 3.77 Hz, 1H), 7.29 (td, J=8.37, 2.20 Hz, 1H), 7.39 (t, J=7.82 Hz, 1H), 7.56-7.66 (m, 3H), 8.07 (t, J=2.20 Hz, 1H), 8.18 (br d, J=7.41 Hz, 1H), 8.41-8.51 (m, 2H), 8.97 (d, J=1.37 Hz, 1H), 9.01 (s, 1H), 9.31 (d, J=1.37 Hz, 1H), 13.45 (br s, 1H); ESIMS found for C₂₉H₂₄FN₇O m/z 506.2 (M+1).

N-(5-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide 945

Brown solid (18.1 mg, 0.03 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.39-1.52 (m, 3H), 1.88 (br d, J=12.90 Hz, 2H), 2.05-2.16 (m, 1H), 2.40 (d, J=6.86 Hz, 2H), 2.85-2.97 (m, 3H), 7.26 (td, J=8.37, 2.74 Hz, 1H), 7.39 (t, J=7.82 Hz, 1H), 7.57-7.66 (m, 3H), 8.19 (br d, J=6.31 Hz, 1H), 8.36 (br d, J=9.33 Hz, 1H), 8.44 (br d, J=11.25 Hz, 1H), 8.60 (br d, J=11.80 Hz, 1H), 8.82 (s, 1H), 8.89 (br s, 1H), 8.99 (s, 2H), 9.31 (d, J=1.10 Hz, 1H), 10.38 (s, 1H), 13.44 (br s, 1H), 14.39 (s, 1H); ESIMS found for C₃₁H₂₇FN₈O m/z 547.3 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl) ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 946

Yellow solid (8 mg, 0.015 mmol, 35.8% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 1.70 (dt, J=6.72, 3.22 Hz, 4H), 2.54-2.60 (m, 4H), 2.88 (t, J=5.76 Hz, 2H), 4.27 (t, J=5.76 Hz, 2H), 7.26 (td, J=8.44, 2.33 Hz, 1H), 7.38 (t, J=7.82 Hz, 1H), 7.56-7.65 (m, 3H), 7.99-8.05 (m, 1H), 8.17 (br d, J=6.59 Hz, 1H), 8.36 (d, J=2.74 Hz, 1H), 8.42 (br d, J=10.43 Hz, 1H), 8.93 (d, J=1.65 Hz, 1H), 8.99 (s, 1H), 9.30 (d, J=1.10 Hz, 1H), 13.41 (brs, 1H), 14.33 (brs, 1H); ESIMS found for C₃₀H₂₆FN₇O m/z 520.2 (M+1).

2-((5-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine 947

Brown solid (29.1 mg, 0.059 mmol, 50.1% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.32 (s, 6H), 2.79 (br t, J=5.49 Hz, 2H), 4.28 (t, J=5.63 Hz, 2H), 7.26 (td, J=8.37, 2.74 Hz, 1H), 7.38 (t, J=7.82 Hz, 1H), 7.60 (dd, J=12.76, 7.27 Hz, 3H), 7.99-8.05 (m, 1H), 8.17 (d, J=7.68 Hz, 1H), 8.37 (d, J=2.74 Hz, 1H), 8.41 (br d, J=11.25 Hz, 1H), 8.94 (d, J=1.37 Hz, 1H), 9.00 (s, 1H), 9.31 (d, J=0.82 Hz, 1H), 13.42 (br s, 1H); ESIMS found for C₂₈H₂₄FN₇O m/z 494.2 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine 948

Yellow solid (31.2 mg, 0.07 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 3.96 (s, 3H), 7.28 (td, J=8.51, 2.47 Hz, 1H), 7.38 (t, J=7.68 Hz, 1H), 7.56-7.65 (m, 3H), 8.00-8.05 (m, 1H), 8.15 (br d, J=6.86 Hz, 1H), 8.37 (d, J=2.74 Hz, 1H), 8.43 (br d, J=11.80 Hz, 1H), 8.93 (d, J=1.65 Hz, 1H), 9.00 (s, 1H), 9.31 (s, 1H), 13.41 (br s, 1H), 14.33 (brs, 1H); ESIMS found for C₂₅H₁₇FN₆O m/z 437.2 (M+1).

5-(3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol 949

Brown solid (17.7 mg, 0.042 mmol, 31.2% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.28 (td, J=8.51, 2.47 Hz, 1H), 7.38 (t, J=7.82 Hz, 1H), 7.55-7.66 (m, 3H), 7.91 (t, J=2.20 Hz, 1H), 8.16-8.23 (m, 2H), 8.43 (br d, J=11.25 Hz, 1H), 8.79 (d, J=1.65 Hz, 1H), 8.98 (s, 1H), 9.30 (d, J=1.37 Hz, 1H), 10.09 (br s, 1H), 13.44 (br s, 1H), 14.13 (br s, 1H); ESIMS found for C₂₄H₁₅FN₆O m/z 423.2 (M+1).

5-(5-(Benzyloxy)pyridin-3-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine 950

Cream colored solid (110 mg, 0.204 mmol, 68.0% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 5.32 (s, 2H), 7.07-7.16 (m, 1H), 7.34-7.41 (m, 2H), 7.42-7.49 (m, 2H), 7.55 (d, J=7.14 Hz, 2H), 7.56-7.64 (m, 3H), 8.07-8.13 (m, 1H), 8.18 (br d, J=7.41 Hz, 1H), 8.40 (br d, J=11.25 Hz, 1H), 8.45 (d, J=2.74 Hz, 1H), 8.97 (d, J=1.37 Hz, 1H), 9.02 (s, 1H), 9.32 (d, J=1.10 Hz, 1H), 13.44 (br s, 1H), 14.35 (br s, 1H); ESIMS found for C₃₁H₂₁FN₆O m/z 513.2 (M+1).

2-Cyclohexyl-N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide 951

White solid (24 mg, 0.042 mmol, 58.5% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.96-1.07 (m, 2H), 1.12-1.33 (m, 3H), 1.63 (br d, J=12.35 Hz, 1H), 1.69 (br d, J=12.90 Hz, 2H), 1.75 (br d, J=10.98 Hz, 2H), 1.83 (ddt, J=14.48, 7.27, 3.67, 3.67 Hz, 1H), 2.29 (d, J=6.86 Hz, 2H), 7.24 (td, J=8.23, 2.47 Hz, 1H), 7.38 (t, J=7.68 Hz, 1H), 7.55-7.67 (m, 3H), 8.19 (br d, J=7.68 Hz, 1H), 8.42 (br d, J=11.80 Hz, 1H), 8.79 (d, J=2.20 Hz, 1H), 8.90 (s, 1H), 8.95-9.01 (m, 2H), 9.32 (d, J=1.10 Hz, 1H), 10.23 (s, 1H), 13.44 (br s, 1H), 14.36 (br s, 1H); ESIMS found for C₃₂H₂₈FN₇O m/z 546.2 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine 952

Off-white solid (80.0 mg, 0.19 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.32 (br t, J=8.51 Hz, 1H), 7.39 (t, J=7.68 Hz, 1H), 7.56-7.68 (m, 3H), 8.06-8.15 (m, 3H), 8.56 (br d, J=11.53 Hz, 1H), 8.74 (br d, J=5.49 Hz, 2H), 9.16 (s, 1H), 9.32 (s, 1H), 13.45 (s, 1H), 14.39 (s, 1H); ESIMS found for C₂₄H₁₅FN₆ m/z 407.1 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine 953

Pink solid (47 mg, 0.110 mmol, 33.7% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm ¹H NMR (499 MHz, DMSO-d₆) δ ppm 7.30 (td, J=8.51, 2.20 Hz, 1H), 7.38 (t, J=7.68 Hz, 1H), 7.44 (ddd, J=7.41, 4.80, 0.96 Hz, 1H), 7.55-7.69 (m, 3H), 7.96 (td, J=7.75, 1.78 Hz, 1H), 8.22 (br d, J=7.68 Hz, 1H), 8.39 (br d, J=10.70 Hz, 1H), 8.45 (d, J=7.96 Hz, 1H), 8.75 (d, J=4.12 Hz, 1H), 9.26 (d, J=1.10 Hz, 1H), 9.63 (s, 1H), 13.42 (br s, 1H), 14.32 (br s, 1H); ESIMS found for C₂₄H₁₅FN₆ m/z 407.1 (M+1).

3-(4-(3-Fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine 954

Cream colored solid (53 mg, 0.130 mmol, 65.9% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 7.29 (td, J=8.51, 2.47 Hz, 1H), 7.39 (t, J=7.68 Hz, 1H), 7.55-7.69 (m, 3H), 8.20 (d, J=7.96 Hz, 1H), 8.39 (br d, J=11.25 Hz, 1H), 8.70 (d, J=2.47 Hz, 1H), 8.78 (s, 1H), 9.32 (d, J=1.10 Hz, 1H), 9.61 (br s, 1H), 9.61 (d, J=1.10 Hz, 1H), 13.46 (br s, 1H), 14.42 (br s, 1H); ESIMS found for C₂₃H₁₄FN₇ m/z 408.1 (M+1).

N-(5-(3-(4-(3-(2-(Dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d] imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1262

Orange solid (10.2 mg, 0.017 mmol, 15.94% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.97 (d, J=6.59 Hz, 6H), 2.07-2.16 (m, 7H), 2.24-2.30 (m, 4H), 4.17 (br t, J=5.90 Hz, 2H), 6.88 (br d, J=10.70 Hz, 1H), 7.37 (t, J=7.68 Hz, 1H), 7.58 (d, J=7.41 Hz, 1H), 7.63 (d, J=7.68 Hz, 1H), 7.85 (s, 1H), 7.89 (br d, J=10.43 Hz, 1H), 8.81-8.86 (m, 2H), 8.92 (d, J=1.92 Hz, 1H), 8.95 (s, 1H), 9.32 (d, J=1.10 Hz, 1H), 10.22 (s, 1H), 13.44 (br s, 1H), 14.38 (br s, 1H); ESIMS found for C₃₃H₃₃FN₈O₂ m/z 593.3 (M+1).

N-(5-(3-(4-(3-Fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d] imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1306

Yellow solid (12.3 mg, 0.020 mmol, 53.7% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.90-1.02 (m, 10H), 1.87 (br s, 1H), 1.99 (br s, 1H), 2.08-2.17 (m, 2H), 2.20-2.26 (m, 1H), 2.28 (d, J=7.14 Hz, 2H), 3.66 (br d, J=4.67 Hz, 2H), 4.46-4.53 (m, 2H), 6.98-7.05 (m, 1H), 7.39 (t, J=7.68 Hz, 1H), 7.58-7.66 (m, 2H), 7.91 (br s, 1H), 7.98 (br d, J=9.61 Hz, 1H), 8.85-8.92 (m, 2H), 8.95-9.02 (m, 2H), 9.33 (d, J=1.37 Hz, 1H), 10.25 (s, 1H), 13.47 (s, 1H), 14.41 (s, 1H); ESIMS found for C₃₅H₃₅FN₈O₂ m/z 619.3 (M+1).

N-(5-(3-(4-(3-Fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1350

Yellow solid (34.2 mg, 0.066 mmol, 83% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.98 (d, J=6.59 Hz, 6H), 2.13 (dquin, J=13.62, 6.75, 6.75, 6.75, 6.75 Hz, 1H), 2.28 (d, J=7.14 Hz, 2H), 6.63 (dt, J=10.50, 2.16 Hz, 1H), 7.36 (t, J=7.68 Hz, 1H), 7.53 (br d, J=7.41 Hz, 1H), 7.57 (br d, J=7.68 Hz, 1H), 7.66 (s, 1H), 7.79 (br d, J=10.43 Hz, 1H), 8.85 (br d, J=11.53 Hz, 2H), 9.01 (s, 2H), 9.32 (s, 1H), 10.01 (s, 1H), 10.18 (s, 1H), 13.40 (s, 1H), 14.36 (s, 1H); ESIMS found for C₂₉H₂₄FN₇O₂ m/z 522.2 (M+1).

N-(5-(3-(4-(3-Fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide 1394

Yellow solid (31.3 mg, 0.058 mmol, 71.0% yield). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 0.98 (d, J=6.59 Hz, 6H), 2.13 (dquin, J=13.70, 6.80, 6.80, 6.80, 6.80 Hz, 1H), 2.28 (d, J=7.14 Hz, 2H), 3.91 (s, 3H), 6.88 (dt, J=10.70, 2.20 Hz, 1H), 7.37 (t, J=7.82 Hz, 1H), 7.58 (br d, J=7.68 Hz, 1H), 7.64 (br d, J=7.41 Hz, 1H), 7.84 (br d, J=10.98 Hz, 1H), 8.03 (br s, 1H), 8.83 (d, J=2.47 Hz, 1H), 8.88 (br s, 1H), 8.98 (d, J=1.65 Hz, 2H), 9.32 (d, J=1.10 Hz, 1H), 10.22 (s, 1H), 13.43 (brs, 1H), 14.37 (brs, 1H); ESIMS found for C₃₀H₂₆FN₇O₂ m/z 536.3 (M+1).

2-(Dimethyl amino)-N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide 1437

Orange solid (18.7 mg, 0.04 mmol). ¹H NMR (DMSO-d₆, 500 MHz) δ ppm 2.37 (s, 6H), 3.23 (br s, 2H), 7.25 (td, J=8.30, 2.61 Hz, 1H), 7.39 (t, J=7.68 Hz, 1H), 7.57-7.66 (m, 3H), 8.19 (d, J=7.96 Hz, 1H), 8.40-8.47 (m, 1H), 8.87 (d, J=2.20 Hz, 1H), 8.91 (t, J=2.20 Hz, 1H), 8.99 (d, J=1.10 Hz, 1H), 9.01 (d, J=1.92 Hz, 1H), 9.32 (d, J=1.37 Hz, 1H), 10.15 (br s, 1H), 13.43 (s, 1H), 14.36 (br s, 1H); ESIMS found for C₂₈H₂₃FN₈O m/z 507.1 (M+1).

Example 2

The screening assay for Wnt activity is described as follows. Reporter cell lines can be generated by stably transducing cancer cell lines (e.g., colon cancer) or primary cells (e.g., IEC-6 intestinal cells) with a lentiviral construct that includes a Wnt-responsive promoter driving expression of the firefly luciferase gene.

SW480 colon carcinoma cells were transduced with a lentiviral vector expressing luciferase with a human Sp5 promoter consisting of a sequence of eight TCF/LEF binding sites. SW480 cells stably expressing the Sp5-Luc reporter gene and a hygromycin resistance gene were selected by treatment with 150 μg/ml of hygromycin for 7 days. These stably transduced SW480 cells were expanded in cell culture and used for all further screening activities. For Sp5-Luc reporter gene assays, the cells were plated at 10,000 cells/well in 96-well plates with growth medium containing 10% fetal calf serum and incubated overnight at 37° C. and 5% CO₂. Each compound was dissolved in DMSO as a 10 mM stock in standard j-vials and used to prepare compound source plates in dose-response format with 3-fold serial dilutions and a 10 mM top concentration. Compound transfer from serially diluted source plates to assay plates containing the cells was accomplished using a pintool (Multimek 96, Beckman equipped with V&P Scientific FP1S50H pins) based liquid handling protocol. This protocol used a slotted pin to transfer 50 nl of compound from a source plate well to an assay plate well containing 50 μl of cells in growth medium. The 1000-fold dilution resulted in a final DMSO concentration of 0.1% on the cells in each well. Control wells received 50 nl of DMSO treatment for normalization and calculating IC₅₀ values. The treated cells were incubated at 37° C. and 5% CO₂ for an additional forty-two hours. Following incubation, the growth medium was removed and 50 μl of BrightGlo luminescence reagent (Promega) was added to each well of the 96-well assay plates. The plates were placed on an orbital shaker for 5 min and then luminescence was quantified on the Victor3 (Perkin Elmer) plate reader. Readings were normalized to DMSO only treated cells, and normalized activities were utilized for IC₅₀ calculations using the dose-response log (inhibitor) vs. response-variable slope (four parameters) nonlinear regression feature available in GraphPad Prism 5.0 or 6.0. Table 2 shows the measured activity for selected compounds of Formula I as described herein.

TABLE 2 Compound IC₅₀ (μM) 1 0.0439 7 0.0424 13 0.0191 20 0.0744 25 0.0873 28 0.0908 34 0.0312 40 0.2869 46 0.0489 52 0.1225 55 0.0195 61 0.0812 68 0.1642 73 0.1233 82 0.0045 87 0.0454 90 0.0139 93 0.5337 101 0.1834 109 0.0425 121 >10 126 >10 136 0.1773 141 0.4111 148 0.1770 154 0.5964 157 0.0972 169 0.0283 175 0.0769 181 0.0871 184 0.0551 190 0.0535 196 0.7454 202 0.0632 211 0.0968 217 0.3554 223 2.186 229 0.6284 234 0.7976 238 0.0077 244 0.2109 250 0.1832 256 0.0956 265 0.0194 268 0.0761 269 0.0042 271 0.0538 277 0.0435 280 0.0062 283 0.0309 288 0.0068 292 0.0296 294 0.0486 295 0.0393 298 0.0129 304 0.1449 306 0.0168 310 0.0588 313 0.1263 319 0.0779 320 0.0566 321 0.0163 325 0.0267 329 0.0560 331 0.0172 337 0.0908 340 0.0173 352 0.0305 358 0.0116 366 0.0311 367 0.0035 373 0.0326 379 0.3590 381 0.0215 385 0.0331 392 0.0321 394 0.0443 400 0.0423 406 0.2471 412 0.1497 421 0.0100 424 0.0199 427 0.1721 433 0.0285 436 0.0258 439 0.0401 442 0.0260 448 0.6713 450 0.1594 454 0.0516 460 0.1824 466 0.4663 937 3.165 938 0.145 939 0.025 940 0.046 941 0.334 942 0.079 943 0.560 944 1.205 945 >10 946 0.580 947 0.463 948 0.033 949 0.029 950 0.165 951 0.115 952 0.053 953 0.250 954 0.045 1262 0.747 1306 1.372 1350 >10 1394 0.183 1437 0.155

Example 3

The above synthesized compounds were screened using primary human mesenchymal stem cells (hMSCs) to determine their ability to induce chondrogenesis (process by which cartilage is developed).

Human Mesenchymal Stem Cell Culture:

Primary human mesenchymal stem cells (hMSCs) were purchased from Lonza (Walkersville, Md.) and expanded in Mesenchymal Stem Cell Growth Media (Lonza). Cells between passage 3 and 6 were used for the experiments.

Compound Screening:

Each compound was dissolved in DMSO as a 10 mM stock and used to prepare compound source plates. Serial dilution (1:3, 6-point dose-response curves from 2700 nM to 10 nM) and compound transfer was performed using the ECHO 550 (Labcyte, Sunnyvale, Calif.) into 96-well clear bottom assay plates (Greiner Bio-One) with appropriate DMSO backfill for a final DMSO concentration of 0.03%. hMSCs were plated at 20,000 cells/well in 250 μL/well Incomplete Chondrogenic Induction Medium (Lonza; DMEM, dexamethasone, ascorbate, insulin-transferrin-selenium [ITS supplement], gentamycin-amphotericin [GA-1000], sodium pyruvate, proline and L-glutamine). TGF-03 (10 ng/mL) was used as a positive control for differentiation while negative control wells were treated with 75 nL DMSO for normalization and calculating EC₅₀ values. Cells were incubated at 37° C. and 5% CO₂ for 6 days. To image chondrogenic nodules, the cells were fixed using 4% formaldehyde (Electron Microscopy Sciences), and stained with 2 μg/mL Rhodamine B (Sigma-Aldrich) and 20 μM Nile Red (Sigma-Aldrich) [Johnson K., et. al, A Stem Cell-Based Approach to Cartilage Repair, Science, (2012), 336(6082), 717-721]. The nodules imaged (4 images per well at 4× magnification) by excitation at 531 nm and emission at 625 nm and quantified using the CellInsight CX5 (Thermo Scientific). Number of nodules in each well was normalized to the average of 3 DMSO treated wells on the same plate using Excel (Microsoft Inc.). The normalized averages (fold change over DMSO) of 3 replicate wells for each compound concentration were calculated. Due to solubility limitations of some of the compounds, curve fitting was incomplete leading to inaccurate EC₅₀ determinations.

Using TGF-β3 as a positive control, the concentration of test compounds required to induce equivalent levels of chondrogenesis is reported. In addition, the maximum activity of each compound and the respective dose that each compound reached maximum chondrogenesis activity is reported. Table 3 shows the activity of selected compounds as provided herein.

TABLE 3 Max. Conc Conc Activity (nM) (nM) as % of 100% of Max. TGF-β3 TGF-β3 Compound activity activity activity 13 300 100 311.4 34 2700 N/A 28.4 55 900 300 105.2 82 30 100 92.9 169 900 N/A 60.3 184 30 N/A 26.7 211 2700 N/A 37.9 238 100 30 80.5 269 900 10 66.3 288 10 N/A 58.0 331 300 N/A 66.8 367 30 30 107.3 392 2700 N/A 29.4 421 100 30 81.8 454 300 300 235.2 939 30 30 70.0

Example 4

The above synthesized compounds were screened using primary human fibroblasts (derived from IPF patients) treated with TGF-β1 to determine their ability to inhibit the fibrotic process.

Human Fibroblast Cell Culture: Primary human fibroblasts derived from IPF patients (LL29 cells) [¹Xiaoqiu Liu, et. al., “Fibrotic Lung Fibroblasts Show Blunted Inhibition by cAMP Due to Deficient cAMP Response Element-Binding Protein Phosphorylation”, Journal of Pharmacology and Experimental Therapeutics (2005), 315(2), 678-687; ²Watts, K. L., et. al., “RhoA signaling modulates cyclin D1 expression in human lung fibroblasts; implications for idiopathic pulmonary fibrosis”, Respiratory Research (2006), 7(1), 88] were obtained from American Type Culture Collection (ATCC) and expanded in F12 medium supplemented with 15% Fetal Bovine Serum and Penicillin/Streptomycin.

Compound Screening: Each compound was dissolved in DMSO as a 10 mM stock and used to prepare compound source plates. Serial dilution (1:2, 11-point dose-response curves from 10 M to 1.87 nM) and compound transfer was performed using the ECHO 550 (Labcyte, Sunnyvale, Calif.) into 384-well clear bottom assay plates (Greiner Bio-One) with appropriate DMSO backfill for a final DMSO concentration of 0.1%. LL29 cells were plated at 1,500 cells/well in 80 l/well F12 medium supplemented with 1% Fetal Bovine Serum. One hour after addition of the cells, TGF-β1 (Peprotech; 20 ng/mL) was added to the plates to induce fibrosis (ref. 1 and 2 above). Wells treated with TGF-β1 and containing DMSO were used as controls. Cells were incubated at 37° C. and 5% CO₂ for 4 days. Following incubation for 4 days, SYTOX green nucleic acid stain (Life Technologies [Thermo Fisher Scientific]) was added to the wells at a final concentration of 1 uM and incubated at room temperature for 30 min. Cells were then fixed using 4% formaldehyde (Electron Microscopy Sciences), washed 3 times with PBS followed by blocking and permeabilization using 3% Bovine Serum Albumin (BSA; Sigma) and 0.3% Triton X-100 (Sigma) in PBS. Cells were then stained with antibody specific to α-smooth muscle actin (αSMA; Abcam) (ref. 1 and 2 above) in 3% Bovine Serum Albumin (BSA; Sigma) and 0.3% Triton X-100 (Sigma) in PBS, and incubated overnight at 4° C. Cells were then washed 3 times with PBS, followed by incubation with Alexa Flor-647 conjugated secondary antibody (Life Technologies [Thermo Fisher Scientific]) and DAPI at room temperature for 1 hour. Cells were then washed 3 times with PBS and plates were sealed for imaging. αSMA staining was imaged by excitation at 630 nm and emission at 665 nm and quantified using the Compartmental Analysis program on the CellInsight CX5 (Thermo Scientific). Dead or apoptotic cells were excluded from analysis based on positive SYTOX green staining. % of total cells positive for αSMA were counted in each well and normalized to the average of 11 wells treated with TGF-β1 on the same plate using Dotmatics' Studies Software. The normalized averages (fold change over untreated) of 3 replicate wells for each compound concentration were used to create dose-responses curves and EC₅₀ values were calculated using non-linear regression curve fit in the Dotmatics' Studies Software. The EC₅₀ values are reported.

Table 4 shows the activity of selected compounds as provided herein.

TABLE 4 Inhibition of fibrosis Compound EC₅₀ (μM) 1 0.009 7 0.017 13 0.009 20 0.010 25 0.048 28 0.741 34 0.047 40 0.078 46 0.094 52 0.147 55 0.009 61 0.081 68 1.672 73 1.189 82 0.009 87 0.124 90 0.009 93 0.009 101 0.009 109 0.029 126 9.990 136 0.122 141 0.039 148 0.225 154 0.009 157 0.099 169 0.009 175 0.187 181 0.009 184 0.277 190 0.009 196 0.009 202 0.009 211 0.613 223 2.613 229 9.990 234 0.009 238 0.057 244 1.960 250 0.258 265 0.009 268 0.009 269 0.049 271 0.009 277 0.009 283 0.009 288 0.009 292 0.009 294 0.009 295 0.249 298 0.009 310 0.083 313 0.455 320 0.096 321 0.306 325 0.009 329 0.067 331 0.029 337 0.009 352 9.990 358 9.990 366 0.224 367 0.009 373 0.014 379 0.009 381 0.079 385 0.020 392 0.267 394 0.110 400 0.218 412 9.176 421 0.009 424 0.051 427 0.009 433 0.009 439 0.009 442 0.009 448 0.009 450 0.085 454 0.117 460 1.057 466 0.308 937 9.990 939 0.009 947 9.990 948 0.072 949 9.990 952 9.990 953 0.009 954 0.253 1350 0.009 1394 0.021 

What is claimed is:
 1. A method of treating a disorder selected from the group consisting of: skin fibrosis; scleroderma; progressive systemic fibrosis; muscle fibrosis; glomerulosclerosis; glomerulonephritis; hypertrophic scar formation; uterine fibrosis; renal fibrosis; cirrhosis of the liver; liver fibrosis; adhesions occurring in the abdomen, pelvis, spine or tendons; chronic obstructive pulmonary disease; fibrosis following myocardial infarction; pulmonary fibrosis; idiopathic pulmonary fibrosis (IPF); fibrosis and scarring associated with diffuse/interstitial lung disease; central nervous system fibrosis; fibrosis following stroke; fibrosis associated with neuro-degenerative disorders selected from the group consisting of Alzheimer's Disease and multiple sclerosis, fibrosis associated with proliferative vitreoretinopathy (PVR); restenosis; endometriosis; ischemic disease; and radiation fibrosis in a subject, the method comprising administering to the subject a therapeutically effective amount of a compound of Formula I, or a pharmaceutically acceptable salt thereof:

wherein: R¹ is selected from the group consisting of -heteroaryl(R⁴)_(q) and -heterocyclyl(R⁵)_(h); R² is selected from the group consisting of H and halide; R³ is selected from the group consisting of H, -heteroaryl(R⁶)_(q), -heterocyclyl(R⁷)_(h), and -aryl(R⁸)_(k); each R⁴ is one substituent attached to the heteroaryl and is independently selected from the group consisting of halide, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R⁹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R¹⁰)_(j), —(C₁₋₄ alkylene)_(p)aryl(R¹¹)_(k), —NHC(═O)R¹², —NR¹³R¹⁴, —(C₁₋₆ alkylene)NR¹⁵R¹⁶, and —OR²²; each R⁵ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN; each R⁶ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —OCH₃, —CN, and —C(═O)R¹⁷; each R⁷ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —CN, and —OCH₃; each R⁸ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₆ alkyl), halide, —CF₃, —CN, —OCH₃, —(C₁₋₆ alkylene)_(p)NHSO₂R¹⁷, —NR¹³(C₁₋₆ alkylene)NR¹³R¹⁴, —(C₁₋₆ alkylene)_(p)NR¹³R¹⁴, and —OR²⁵; each R⁹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of amino, —(C₁₋₄ alkyl), halide, —CF₃, and —CN; each R¹⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN; each R¹¹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN; each R¹² is independently selected from the group consisting of —(C₁₋₉ alkyl), -heteroaryl(R¹⁸)_(q), -aryl(R¹⁹)_(k), —CH₂aryl(R¹⁹)_(k), -carbocyclyl(R²⁰)_(j), —CH₂carbocyclyl(R²⁰)_(j), —(C₁₋₄ alkylene)_(p)NR²³R²⁴, -heterocyclyl(R²¹)_(h), and —CH₂heterocyclyl(R²¹)_(h); each R¹³ is independently selected from the group consisting of H and —(C₁₋₆ alkyl); each R¹⁴ is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —CH₂aryl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰)_(j); each R¹⁵ is independently selected from the group consisting of H and —(C₁₋₆ alkyl); each R¹⁶ is independently selected from the group consisting of H, —(C₁₋₆ alkyl), —CH₂aryl(R¹⁹)_(k), and —CH₂carbocyclyl(R²⁰)_(j); each R¹⁷ is a —(C₁₋₆ alkyl); each R¹⁸ is one substituent attached to the heteroaryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN; each R¹⁹ is one substituent attached to the aryl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN; each R²⁰ is one substituent attached to the carbocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN; each R²¹ is one substituent attached to the heterocyclyl and is independently selected from the group consisting of —(C₁₋₄ alkyl), halide, —CF₃, and —CN; R²² is selected from the group consisting of H, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R²¹)_(h), —(C₁₋₄ alkylene)_(p)carbocyclyl(R²⁰), —(C₁₋₄ alkylene)_(p)aryl(R¹⁹)_(k), and —(C₁₋₆ alkylene)_(p)NR²³R²⁴; each R²³ is independently selected from the group consisting of H and —(C₁₋₆ alkyl); each R²⁴ is independently selected from the group consisting of H and —(C₁₋₆ alkyl); R²⁵ is selected from the group consisting of H, —(C₁₋₆ alkyl), —(C₁₋₄ alkylene)_(p)heterocyclyl(R²¹)_(h), and —(C₁₋₆ alkylene)_(p)NR²³R²⁴; each p is independently 0 or 1; each q is independently 0 to 4; each h is independently 0 to 10; each k is independently 0 to 5; and each j is independently 0 to
 12. 2. The method of claim 1, wherein R¹ is -heteroaryl(R⁴)_(q).
 3. The method of claim 2, wherein R¹ is selected from the group consisting of -pyridinyl(R⁴), -pyrimidinyl(R⁴)_(q), -pyrazolyl(R⁴)_(q), and -imidazolyl(R⁴)_(q).
 4. The method of claim 2, wherein q is 0, 1 or
 2. 5. The method of claim 4, wherein R⁴ is selected from the group consisting of —(C₁₋₃ alkyl), —CH₂heterocyclyl(R⁹)_(h), —NHC(═O)R¹², —NR¹³R¹⁴, —CH₂NR¹⁵R¹⁶, and —OR²².
 6. The method of claim 1, wherein R³ is -aryl(R⁸)_(k).
 7. The method of claim 6, wherein R³ is -phenyl(R⁸)_(k).
 8. The method of claim 6, wherein k is 1, or
 2. 9. The method of claim 8, wherein each R⁸ is independently selected from the group consisting of halide and is —CH₂NHSO₂R¹⁷.
 10. The method of claim 1, wherein R³ is -heteroaryl(R⁶)_(q).
 11. The method of claim 10, wherein R³ is selected from the group consisting of -pyridinyl(R⁶)_(q), -imidazolyl(R⁶)_(q), -furanyl(R⁶)_(q), and -thiophenyl(R⁶)_(q).
 12. The method of claim 10, wherein q is 0 or
 1. 13. The method of claim 12, wherein q is 1 and R⁶ is selected from the group consisting of halide, —(C₁₋₃ alkyl), and —C(═O)R¹⁷.
 14. The method of claim 1, wherein R³ is -heterocyclyl(R⁷)_(h).
 15. The method of claim 14, wherein R³ is selected from the group consisting of -piperidinyl(R⁷)_(h), -morpholinyl(R⁷)_(h), and -piperazinyl(R⁷)_(h).
 16. The method of claim 14, wherein h is 0, 1, or
 2. 17. The method of claim 16, wherein each R⁷ is independently selected from the group consisting of a halide and —(C₁₋₃ alkyl).
 18. The method of claim 1, wherein R² is H.
 19. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (1); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (2); 5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (3); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (4); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (5); N-((5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (6); 5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (7); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (8); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (9); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (10); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (11); 5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (12); 1-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (13); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (14); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (15); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (16); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (17); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (18); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (19); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (20); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (21); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (22); N-benzyl-1-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (23); 1-cyclopentyl-N-((5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (24); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (25); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (26); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (27); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (28); 5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (29); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (30); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (31); N-((5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (32); 5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (33); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (34); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (35); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (36); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (37); 5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (38); 1-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (39); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (40); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (41); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (42); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (43); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (44); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (45); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (46); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (47); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (48); N-benzyl-1-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (49); 1-cyclopentyl-N-((5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (50); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (51); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (52); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (53); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (54); 5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (55); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (56); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (57); N-((5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (58); 5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (59); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (60); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (61); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (62); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (63); 5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (64); 1-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (65); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (66); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (67); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (68); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (69); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (70); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (71); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (72); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (73); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (74); N-benzyl-1-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (75); 1-cyclopentyl-N-((5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (76); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (77); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (78); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (79); 3-methyl-N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (80); 5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (81); 5-(pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (82); 5-(4-methylpyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (83); N-((5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (84); N,N-dimethyl-5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (85); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (86); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (87); 2-phenyl-N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (88); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (89); N-isopropyl-5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (90); N,N-dimethyl-1-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (91); 3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (92); 5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (93); 3,3-dimethyl-N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (94); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (95); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (96); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (97); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (98); N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (99); and N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (100); or a pharmaceutically acceptable salt thereof.
 20. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-benzyl-1-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (101); 1-cyclopentyl-N-((5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (102); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (103); 3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (104); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (105); 3-methyl-N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (106); 5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (107); 5-(pyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (108); 5-(4-methylpyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (109); N-((5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (110); N,N-dimethyl-5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (111); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (112); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (113); 2-phenyl-N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (114); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (115); N-isopropyl-5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (116); N,N-dimethyl-1-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (117); 3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (118); 5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (119); 3,3-dimethyl-N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (120); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (121); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (122); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (123); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (124); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (125); N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (126); N-benzyl-1-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (127); 1-cyclopentyl-N-((5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (128); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (129); 3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (130); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (131); 3-methyl-N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (132); 5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (133); 3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (134); 5-(4-methylpyridin-3-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (135); N-((5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (136); N,N-dimethyl-5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (137); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (138); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (139); 2-phenyl-N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (140); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (141); N-isopropyl-5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (142); N,N-dimethyl-1-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (143); 3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (144); 5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (145); 3,3-dimethyl-N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (146); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (147); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (148); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (149); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (150); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (151); N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (152); N-benzyl-1-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (153); 1-cyclopentyl-N-((5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (154); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (155); 3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (156); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (157); 3-methyl-N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (158); 5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (159); 3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrdin-3-yl)-1H-pyrazolo[3,4-c]pyridine (160); 5-(4-methylpyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (161); N-((5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (162); N,N-dimethyl-5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (163); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (164); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (165); 2-phenyl-N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (166); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (167); N-isopropyl-5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (168); N,N-dimethyl-1-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (169); 3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (170); 3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (171); 3,3-dimethyl-N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (172); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (173); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (174); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (175); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (176); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (177); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (178); N-benzyl-1-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (179); 1-cyclopentyl-N-((5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (180); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (181); 3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (182); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (183); 3-methyl-N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (184); 5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (185); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (186); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (187); N-((5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (188); N,N-dimethyl-5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (189); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (190); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (191); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (192); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (193); N-isopropyl-5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (194); N,N-dimethyl-1-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (195); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (196); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (197); 3,3-dimethyl-N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (198); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (199); and N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (200); or a pharmaceutically acceptable salt thereof.
 21. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (201); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (202); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (203); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (204); N-benzyl-1-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (205); 1-cyclopentyl-N-((5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (206); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(4-methyl)pyridin-3-yl)-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (207); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo [3,4-c]pyridine (208); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (209); 3-methyl-N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (210); 5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (211); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (212); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (213); N-((5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (214); N,N-dimethyl-5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (215); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (216); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (217); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (218); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (219); N-isopropyl-5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (220); N,N-dimethyl-1-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (221); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (222); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (223); 3,3-dimethyl-N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (224); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (225); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (226); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (227); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (228); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (229); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (230); N-benzyl-1-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (231); 1-cyclopentyl-N-((5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (232); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo [d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (233); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (234); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (235); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (236); 5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (237); 3-(1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (238); 3-(1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (239); N-((5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (240); 5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (241); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (242); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (243); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (244); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (245); 5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (246); 1-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (247); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (248); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (249); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (250); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (251); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (252); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (253); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (254); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (255); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (256); 1-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N-benzylmethanamine (257); 1-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N-(cyclopentylmethyl)methanamine (258); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (259); 3-(1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (260); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (261); 3-methyl-N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (262); 5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (263); 5-(pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (264); 5-(4-methylpyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (265); N-((5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (266); N,N-dimethyl-5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (267); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (268); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (269); 2-phenyl-N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (270); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (271); N-isopropyl-5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (272); N,N-dimethyl-1-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (273); 5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (274); 5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (275); 3,3-dimethyl-N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (276); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (277); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (278); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (279); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (280); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (281); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (282); N-benzyl-1-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (283); 1-cyclopentyl-N-((5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (284); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (285); 5-(pyrimidin-5-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (286); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (287); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (288); 5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (289); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (290); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (291); N-((5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (292); 5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (293); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (294); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (295); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (296); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (297); 5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (298); 1-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (299); and 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (300); or a pharmaceutically acceptable salt thereof.
 22. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (301); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (302); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (303); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (304); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (305); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (306); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (307); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (308); N-benzyl-1-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (309); 1-cyclopentyl-N-((5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (310); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (311); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (312); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (313); 3-methyl-N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazzo-2-yl)-1H-pyrazolo[3,4-c]pyyridin-5-yl)pyridin-3-yl)butanamide (314); 5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (315); 5-(pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (316); 5-(4-methylpyridin-3-yl)-3-(4-(thiophen-2-yl)-H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (317); N-((5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (318); N,N-dimethyl-5-(3-(4-(thiophen-2-yl)-H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (319); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (320); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (321); 2-phenyl-N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (322); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (323); N-isopropyl-5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (324); N,N-dimethyl-1-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (325); 5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (326); 5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (327); 3,3-dimethyl-N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (328); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (329); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (330); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (331); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (332); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (333); N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (334); N-benzyl-1-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (335); 1-cyclopentyl-N-((5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (336); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (337); 5-(pyrimidin-5-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (338); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (339); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (340); 5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (341); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (342); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (343); N-((5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (344); 5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (345); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (346); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (347); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (348); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (349); 5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (350); 1-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (351); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (352); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (353); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (354); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (355); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (356); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (357); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (358); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (359); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (360); N-benzyl-1-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (361); 1-cyclopentyl-N-((5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (362); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(5-fluorothiophen-2-yl)-1H-benzo [d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (363); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (364); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (365); 3-methyl-N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (366); 5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (367); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (368); 5-(4-methylpyridin-3-yl)-3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (369); N-((5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (370); N,N-dimethyl-5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (371); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (372); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (373); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (374); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (375); N-isopropyl-5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (376); N,N-dimethyl-1-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (377); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(378); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(379); 3,3-dimethyl-N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butanamide (380); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (381); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (382); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (383); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (384); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (385); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (386); N-benzyl-1-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (387); 1-cyclopentyl-N-((5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (388); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(389); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (390); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (391); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (392); 1-(5-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (393); 1-(5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (394); 1-(5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (395); 1-(5-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (396); 1-(5-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (397); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (398); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (399); and N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (400); or a pharmaceutically acceptable salt thereof.
 23. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (401); 1-(5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (402); 1-(5-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (403); 1-(5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (404); 1-(5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (405); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (406); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (407); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (408); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (409); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (410); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (411); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (412); 1-(5-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (413); 1-(5-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (414); 1-(5-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (415); 1-(5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (416); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (417); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (418); N-(3-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (419); N-(3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (420); N-(3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methane sulfonamide (421); N-(3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (422); N-(3-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (423); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (424); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (425); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (426); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (427); N-(3-fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)benzyl)methanesulfonamide (428); N-(3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (429); N-(3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methane sulfonamide (430); N-(3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methane sulfonamide (431); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (432); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (433); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (434); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (435); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (436); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (437); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (438); N-(3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (439); N-(3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (440); N-(3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (441); N-(3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (442); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (443); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (444); N¹-(3-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (445); N¹-(3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (446); N¹-(3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (447); N¹-(3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (448); N¹-(3-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (449); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (450); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (451); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (452); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (453); N¹-(3-fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (454); N¹-(3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (455); N¹-(3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (456); N¹-(3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (457); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (458); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (459); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (460); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (461); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (462); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (463); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (464); N¹-(3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (465); N¹-(3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (466); N¹-(3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (467); and N¹-(3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (468); or a pharmaceutically acceptable salt thereof.
 24. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (937); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (938); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (939); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (940); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (941); 1-(6-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (942); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (943); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (944); N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (945); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(946); 2-((5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (947); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (948); 5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (949); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (950); 2-cyclohexyl-N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (951); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (952); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (953); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (954); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (955); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (956); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (957); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (958); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (959); 1-(6-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (960); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (961); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (962); N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (963); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(964); 2-((5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (965); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (966); 5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (967); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (968); 2-cyclohexyl-N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (969); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (970); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (971); 3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (972); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (973); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (974); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (975); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (976); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (977); 1-(6-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (978); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (979); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (980); N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (981); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(982); 2-((5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (983); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (984); 5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (985); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (986); 2-cyclohexyl-N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (987); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (988); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (989); 3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (990); 5-(piperidin-4-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (991); 3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (992); 5-(1H-pyrazol-4-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (993); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (994); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (995); 1-(6-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (996); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (997); 5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (998); 2-(piperidin-4-yl)-N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (999); and 3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1000); or a pharmaceutically acceptable salt thereof.
 25. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N,N-dimethyl-2-((5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1001); 5-(5-methoxypyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1002); 5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1003); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1004); 2-cyclohexyl-N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1005); 3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1006); 5-(pyridin-2-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1007); 5-(pyrazin-2-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1108); 5-(piperidin-4-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1009); 3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (1010); 5-(1H-pyrazol-4-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1011); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1012); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1013); 1-(6-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1014); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1015); 5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1016); 2-(piperidin-4-yl)-N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1017); 3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1018); N,N-dimethyl-2-((5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1019); 5-(5-methoxypyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1020); 5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1021); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1022); 2-cyclohexyl-N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1023); 5-(pyridin-4-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1024); 5-(pyridin-2-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1025); 5-(pyrazin-2-yl)-3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1026); 5-(piperidin-4-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1027); 3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (1028); 5-(1H-pyrazol-4-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1029); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1030); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1031); 1-(6-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1032); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1033); 5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1034); 2-(piperidin-4-yl)-N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1035); 3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1036); N,N-dimethyl-2-((5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1037); 5-(5-methoxypyridin-3-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1038); 5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1039); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1040); 2-cyclohexyl-N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1041); 3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1042); 5-(pyridin-2-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1043); 5-(pyrazin-2-yl)-3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1044); 3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1045); 3-(4-(piperidin-1-yl)-H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (1046); 3-(4-(piperidin-1-yl)-H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (1047); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1048); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1049); 1-(6-(3-(4-(piperidin-1-yl)-H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1050); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1051); 3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1052); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1053); 3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1054); N,N-dimethyl-2-((5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1055); 5-(5-methoxypyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1056); 5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1057); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1058); 2-cyclohexyl-N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1059); 3-(4-(piperidin-1-yl)-H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1060); 3-(4-(piperidin-1-yl)-H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1061); 3-(4-(piperidin-1-yl)-H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1062); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo [3,4-c]pyridine (1063); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1064); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (1065); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (1066); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1067); 1-(6-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1068); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1069); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1070); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1071); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1072); N,N-dimethyl-2-((5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1073); 5-(5-methoxypyridin-3-yl)-3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1074); 5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1075); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1076); 2-cyclohexyl-N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1077); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1078); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1079); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1080); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1081); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (1082); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (1083); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1084); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1085); 1-(6-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1086); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1087); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1088); N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1089); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1090); N,N-dimethyl-2-((5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1091); 5-(5-methoxypyridin-3-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1092); 5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1093); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1094); 2-cyclohexyl-N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1095); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1096); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1097); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1098); 3-(1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1099); and 3-(1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1100); or a pharmaceutically acceptable salt thereof.
 26. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 3-(1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine(1101); 3-(1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine(1102); 3-(1H-benzo[d]imidazol-2-yl)-5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-c]pyridine (1103); 1-(6-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1104); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (1105); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (1106); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1107); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (1108); 2-((5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (1109); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1110); 5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1111); 3-(1H-benzo[d]imidazol-2-yl)-5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1112); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-cyclohexylacetamide (1113); 3-(1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1114); 3-(1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine(1115); 3-(1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine(1116); 5-(piperidin-4-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1117); 5-(1,2,3,6-tetrahydropyridin-4-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1118); 5-(1H-pyrazol-4-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1119); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1120); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1121); 1-(6-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1122); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1123); 5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1124); 2-(piperidin-4-yl)-N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1125); 5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1126); N,N-dimethyl-2-((5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1127); 5-(5-methoxypyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1128); 5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1129); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1130); 2-cyclohexyl-N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1131); 5-(pyridin-4-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1132); 5-(pyridin-2-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1133); 5-(pyrazin-2-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1134); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1135); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1136); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (1137); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (1138); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1139); 1-(6-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1140); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1141); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1142); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1143); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1144); 2-((5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (1145); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (1146); 5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1147); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1148); 2-cyclohexyl-N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1149); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1150); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine(1151); 3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1152); 5-(piperidin-4-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1153); 5-(1,2,3,6-tetrahydropyridin-4-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1154); 5-(1H-pyrazol-4-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1155); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1156); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1157); 1-(6-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1158); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1159); 5-(5-(piperidin-4-yloxy)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1160); 2-(piperidin-4-yl)-N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1161); 5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1162); N,N-dimethyl-2-((5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1163); 5-(5-methoxypyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1164); 5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1165); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1166); 2-cyclohexyl-N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1167); 5-(pyridin-4-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1168); 5-(pyridin-2-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1169); 5-(pyrazin-2-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1170); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1171); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (1172); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (1173); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (1174); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1175); 1-(6-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1176); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1177); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1178); N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1179); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1180); 2-((5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (1181); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1182); 5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1183); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1184); 2-cyclohexyl-N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1185); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1186); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1187); 3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1188); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1189); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo [3,4-c]pyridine (1190); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (1191); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1192); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1193); 1-(6-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1194); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1195); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1196); N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1197); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1198); N,N-dimethyl-2-((5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)ethan-1-amine (1199); and 5-(5-methoxypyridin-3-yl)-3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1200); or a pharmaceutically acceptable salt thereof.
 27. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1201); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1202); 2-cyclohexyl-N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1203); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1204); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1205); 3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1206); 1-(5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1207); 1-(5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1208); 1-(5-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1209); 1-(5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1210); 1-(5-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1211); 1-(5-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1212); 1-(5-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1213); 1-(5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1214); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1215); 1-(5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1216); 1-(5-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1217); 1-(5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1218); 1-(5-(2-(5-(5-hydroxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1219); 1-(5-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1220); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-cyclohexylacetamide (1221); 1-(5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1222); 1-(5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1223); 1-(5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)thiophen-2-yl)ethan-1-one (1224); N-(3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (1225); N-(3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)benzyl)methanesulfonamide (1226); N-(3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (1227); N-(3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)benzyl)methanesulfonamide (1228); N-(3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (1229); N-(3-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (1230); N-(3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (1231); N-(3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)benzyl)methanesulfonamide (1232); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1233); N-(3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (1234); N-(3-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (1235); N-(3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)benzyl)methanesulfonamide (1236); N-(3-fluoro-5-(2-(5-(5-hydroxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)benzyl)methanesulfonamide (1237); N-(3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (1238); 2-cyclohexyl-N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1239); N-(3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (1240); N-(3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (1241); N-(3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (1242); N¹-(3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1243); N¹-(3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1244); N₁-(3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (1245); N¹-(3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1246); N¹-(3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (1247); N¹-(3-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (1248); N¹-(3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (1249); N¹-(3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1250); N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1251); N¹-(3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1252); N¹-(3-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (1253); N¹-(3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1254); 5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-ol (1255); N¹-(3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (1256); 2-cyclohexyl-N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo [d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1257); N¹-(3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1258); N¹-(3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1259); N¹-(3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenyl)-N²,N²-dimethylethane-1,2-diamine (1260); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (1261); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (1262); 5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-amine (1263); 2-(3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1264); 2-(3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1265); 2-(3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1266); 5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (1267); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (1268); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (1269); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (1270); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (1271); 5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (1272); 2-(3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1273); 2-(3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1274); 2-(3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1275); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (1276); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (1277); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (1278); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (1279); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (1280); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (1281); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (1282); 2-(3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1283); 2-(3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1284); 2-(3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1285); 2-(3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1286); 2-(3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1287); 2-(3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1288); 2-(3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1289); 2-(3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1290); 2-(3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1291); 2-(3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1292); 1-(6-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1293); 2-(3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1294); 2-(3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1295); N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1296); 2-(3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1297); 2-((5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (1298); 2-(3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1299); and 5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-ol (1300); or a pharmaceutically acceptable salt thereof.
 28. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: 2-(3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenoxy)-N,N-dimethylethan-1-amine (1301); 2-cyclohexyl-N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1302); 2-(3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1303); 2-(3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenoxy)-N,N-dimethylethan-1-amine (1304); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (1305); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (1306); 5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-amine (1307); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1308); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1309); N-((5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (1310); 5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (1311); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (1312); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (1313); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (1314); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (1315); 5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (1316); 1-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (1317); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1318); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1319); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (1320); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (1321); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (1322); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (1323); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (1324); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (1325); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (1326); N-benzyl-1-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (1327); 1-cyclopentyl-N-((5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (1328); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1329); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine(1330); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine(1331); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1332); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1333); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine(1334); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine(1335); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1336); 1-(6-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1337); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1338); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1339); N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1340); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1341); 2-((5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (1342); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1343); 5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-ol (1344); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1345); 2-cyclohexyl-N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1346); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1347); 3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine(1348); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (1349); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (1350); 3-(2-(5-(5-aminopyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1351); 3-fluoro-5-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1352); 3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1353); 3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorophenol (1354); 3-(2-(5-(5-(dimethylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1355); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (1356); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (1357); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (1358); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (1359); 3-fluoro-5-(2-(5-(5-(isopropylamino)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenol (1360); 3-(2-(5-(5-((dimethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1361); 3-fluoro-5-(2-(5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenol (1362); 3-fluoro-5-(2-(5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenol (1363); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-indazol-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (1364); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (1365); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (1366); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (1367); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (1368); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (1369); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (1370); 3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1371); 3-(2-(5-(5-(((cyclopentylmethyl)amino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1372); 3-(2-(5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1373); 3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1374); 3-fluoro-5-(2-(5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1375); 3-fluoro-5-(2-(5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1376); 3-fluoro-5-(2-(5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenol (1377); 3-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1378); 3-fluoro-5-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenol (1379); 3-(2-(5-(1,2-dimethyl-1H-imidazol-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1380); 3-(2-(5-(6-(3-aminoazetidin-1-yl)pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1381); 3-(2-(5-(5-(cyclohexyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1382); 3-fluoro-5-(2-(5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenol (1383); N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1384); 3-fluoro-5-(2-(5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)phenol (1385); 3-(2-(5-(5-(2-(dimethylamino)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorophenol (1386); 3-fluoro-5-(2-(5-(5-methoxypyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1387); 5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1388); 3-(2-(5-(5-(benzyloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenol (1389); 2-cyclohexyl-N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1390); 3-fluoro-5-(2-(5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1391); 3-fluoro-5-(2-(5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)phenol (1392); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (1393); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (1394); 5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (1395); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (1396); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(4-methylpyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1397); N-((5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (1398); 5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N,N-dimethylpyridin-3-amine (1399); and N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (1400); or a pharmaceutically acceptable salt thereof.
 29. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)isobutyramide (1401); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-phenylacetamide (1402); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (1403); 5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (1404); 1-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (1405); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1406); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1407); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3,3-dimethylbutanamide (1408); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (1409); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pentanamide (1410); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopropanecarboxamide (1411] N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (1412); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclopentanecarboxamide (1413); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclohexanecarboxamide (1414); N-benzyl-1-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (1415); 1-cyclopentyl-N-((5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (1416); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1417); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo [3,4-c]pyridine (1418); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1419); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(piperidin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1420); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(1,2,3,6-tetrahydropyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine(1421); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (1422); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (1423); 5-(1,2-dimethyl-1H-imidazol-5-yl)-3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1424); 1-(6-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyrazin-2-yl)azetidin-3-amine (1425); 5-(5-(cyclohexyloxy)pyridin-3-yl)-3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1426); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-4-yloxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1427); N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(piperidin-4-yl)acetamide (1428); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-(2-(pyrrolidin-1-yl)ethoxy)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(1429); 2-((5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)oxy)-N,N-dimethylethan-1-amine (1430); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(5-methoxypyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1431); 5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-ol (1432); 5-(5-(benzyloxy)pyridin-3-yl)-3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1433); 2-cyclohexyl-N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1434); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-4-yl)-1H-pyrazolo[3,4-c]pyridine (1435); 3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyrazin-2-yl)-1H-pyrazolo[3,4-c]pyridine (1436); 2-(dimethylamino)-N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1437); 2-(dimethylamino)-N-(5-(3-(4-(4-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1438); 2-(dimethylamino)-N-(5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1439); 2-(dimethylamino)-N-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1440); 2-(dimethylamino)-N-(5-(3-(4-(pyridin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1441); 2-(dimethylamino)-N-(5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1442); 2-(dimethylamino)-N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1443); 2-(dimethylamino)-N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1444); 2-(dimethylamino)-N-(5-(3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1445); N-(5-(3-(1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(dimethylamino)acetamide (1446); 2-(dimethylamino)-N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1447); 2-(dimethylamino)-N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1448); 2-(dimethylamino)-N-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1449); 2-(dimethylamino)-N-(5-(3-(4-(5-fluorothiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1450); 2-(dimethylamino)-N-(5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1451); N-(5-(3-(4-(5-acetylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-2-(dimethylamino)acetamide (1452); 2-(dimethylamino)-N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo [d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1453); 2-(dimethylamino)-N-(5-(3-(4-(3-((2-(dimethylamino)ethyl)amino)-5-fluorophenyl)-1H-benzo [d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1454); 2-(dimethylamino)-N-(5-(3-(4-(3-(2-(dimethylamino)ethoxy)-5-fluorophenyl)-1H-benzo [d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1455); 2-(dimethylamino)-N-(5-(3-(4-(3-fluoro-5-(2-(pyrrolidin-1-yl)ethoxy)phenyl)-1H-benzo [d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1456); 2-(dimethylamino)-N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1457); 2-(dimethylamino)-N-(5-(3-(4-(3-fluoro-5-methoxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)acetamide (1458); 4-(2-(5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)morpholine (1459); 3-(4-(4,4-difluoropiperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (1460); 3-(4-(1-methylpiperidin-4-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine (1461); 4-(2-(5-(5-fluoropyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl) morpholine (1462); 5-(5-fluoropyridin-3-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1463); 3-(4-(4,4-difluoropiperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-fluoropyridin-3-yl)-1H-pyrazolo [3,4-c]pyridine (1464); 5-(5-fluoropyridin-3-yl)-3-(4-(1-methylpiperidin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1465); 5-(5-fluoropyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (1466); 4-(2-(5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl) morpholine (1467); 3-(4-(4,4-difluoropiperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(1-methyl-1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine (1468); 5-(1-methyl-1H-pyrazol-4-yl)-3-(4-(1-methylpiperidin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1469); 4-(2-(5-(1-cyclopropyl-1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)morpholine (1470); 5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1471); 5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(4-(4,4-difluoropiperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(1472); 5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(4-(1-methylpiperidin-4-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1473); 5-(1-cyclopropyl-1H-pyrazol-4-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (1473); 4-(2-(5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl) morpholine (1474); 3-(4-(4,4-difluoropiperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo [3,4-c]pyridine(1475); and 3-(4-(1-methylpiperidin-4-yl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (1476); or a pharmaceutically acceptable salt thereof.
 30. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of: N-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (1); 1-(5-(3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-N,N-dimethylmethanamine (13); 5-(3-(4-(2-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (55); 5-(pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(82); N-isopropyl-5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine (90); 5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridine (93); N-benzyl-1-(5-(3-(4-(pyridin-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (101); 1-cyclopentyl-N-((5-(3-(4-(pyridin-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)methanamine (154); N-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)propionamide (157); N,N-dimethyl-1-(5-(3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (169); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(piperidin-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine(181); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (190); 3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(5-(pyrrolidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine(196); N-(5-(3-(4-(4-methyl-1H-imidazol-1-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)cyclobutanecarboxamide (202); 3-(4-(4-methylpiperazin-1-yl)-1H-benzo[d]imidazol-2-yl)-5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridine (234); 5-(4-methylpyridin-3-yl)-3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine (265); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (268); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (271); N-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (277); N-benzyl-1-(5-(3-(4-(thiophen-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine (283); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (288); N-((5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methyl)ethanamine (292); N-(5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)pivalamide (294); 5-(3-(4-(furan-3-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)-N-isopropylpyridin-3-amine (298); N,N-dimethyl-1-(5-(3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)methanamine [325); 5-(5-((3,3-difluoropyrrolidin-1-yl)methyl)pyridin-3-yl)-3-(4-(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridine[337); 5-(3-(4-(5-methylthiophen-2-yl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-amine [367); 3-(4-(5-methylthiophen-2-yl)-H-benzo[d]imidazol-2-yl)-5-(5-(piperidin-1-ylmethyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridine[379); N-(3-fluoro-5-(2-(5-(4-methylpyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methane sulfonamide (421); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)benzamide (427); N-(5-(3-(4-(3-fluoro-5-(methylsulfonamidomethyl)phenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo [3,4-c]pyridin-5-yl)pyridin-3-yl)butyramide (433); N-(3-(2-(5-(5-((benzylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo [d]imidazol-4-yl)-5-fluorobenzyl)methanesulfonamide (439); N-(3-fluoro-5-(2-(5-(pyrimidin-5-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)benzyl)methanesulfonamide (442); N¹-(3-(2-(5-(5-((ethylamino)methyl)pyridin-3-yl)-1H-pyrazolo[3,4-c]pyridin-3-yl)-1H-benzo[d]imidazol-4-yl)-5-fluorophenyl)-N²,N²-dimethylethane-1,2-diamine (448); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(1H-pyrazol-4-yl)-1H-pyrazolo[3,4-c]pyridine (939); 3-(4-(3-fluorophenyl)-1H-benzo[d]imidazol-2-yl)-5-(pyridin-2-yl)-1H-pyrazolo[3,4-c]pyridine (953); and N-(5-(3-(4-(3-fluoro-5-hydroxyphenyl)-1H-benzo[d]imidazol-2-yl)-1H-pyrazolo[3,4-c]pyridin-5-yl)pyridin-3-yl)-3-methylbutanamide (1350); or a pharmaceutically acceptable salt thereof.
 31. The method of claim 1, wherein the compound of Formula I is selected from the group consisting of:

or a pharmaceutically acceptable salt thereof.
 32. The method of claim 1, wherein the fibrotic disorder is a skin fibrosis.
 33. The method of claim 1, wherein the fibrotic disorder is scleroderma.
 34. The method of claim 1, wherein the fibrotic disorder is pulmonary fibrosis.
 35. The method of claim 1, wherein the fibrotic disorder is renal fibrosis.
 36. The method of claim 1, wherein the fibrotic disorder is idiopathic pulmonary fibrosis (IPF).
 37. The method of claim 1, wherein the fibrotic disorder is cirrhosis of the liver.
 38. The method of claim 1, wherein the fibrotic disorder is liver fibrosis.
 39. The method of claim 1, wherein the subject is a human. 